(Circular) (Six-base) MAPSORT of: pFN21AE1515.seq Check: 65 from: 1 to: 5485 pFN21AE1515 5485 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2577 3295 278 Size: 53 83 186 936 1041 718 2468 Fragments arranged by size: 2468 1041 936 718 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 3548 4932 5200 1632 Size: 402 1514 1384 268 1917 Fragments arranged by size: 1917 1514 1384 402 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 4384 5361 1203 Size: 3181 977 1327 Fragments arranged by size: 3181 1327 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 2113 4214 2113 Size: 2101 3384 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5485 AlwNI CAG_nnn'CTG Cuts at: 2483 3228 4800 2483 Size: 745 1572 3168 Fragments arranged by size: 3168 1572 745 ApaI G_GGCC'C Cuts at: 2773 2781 2773 Size: 8 5477 ApaLI G'TGCA_C Cuts at: 3542 4698 3542 Size: 1156 4329 ApoI r'AATT_y Cuts at: 1493 2171 2794 3020 3172 5281 1493 Size: 678 623 226 152 2109 1697 Fragments arranged by size: 2109 1697 678 623 226 152 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2766 2810 1870 Size: 73 823 44 4545 Fragments arranged by size: 4545 823 73 44 BamHI G'GATC_C Cuts at: 2815 3182 2815 Size: 367 5118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2723 2806 4261 618 Size: 325 217 627 936 83 1455 1842 Fragments arranged by size: 1842 1455 936 627 325 217 83 BanII G_rGCy'C Cuts at: 1391 2056 2773 2781 2804 1391 Size: 665 717 8 23 4072 Fragments arranged by size: 4072 717 665 23 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2022 928 Size: 971 123 4391 Fragments arranged by size: 4391 971 123 BbvCI CC'TCA_GC Cuts at: 2563 2563 Size: 5485 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3676 3710 1903 Size: 34 50 34 1655 34 3678 Fragments arranged by size: 3678 1655 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4587 3377 Size: 1210 4275 BclI T'GATC_A Cuts at: 1548 1548 Size: 5485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 2709 4096 Size: 107 122 71 631 605 430 607 1387 Cuts at: 4096 136 Size: 1525 Fragments arranged by size: 1525 1387 631 607 605 430 122 107 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2902 2902 Size: 5485 Bme1580I G_kGCm'C Cuts at: 1790 2562 2686 2726 2773 2781 3546 4702 1790 Size: 772 124 40 47 8 765 1156 2573 Fragments arranged by size: 2573 1156 772 765 124 47 40 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2608 2638 3216 Size: 416 53 83 399 24 1191 30 578 Cuts at: 3216 4174 442 Size: 958 1753 Fragments arranged by size: 1753 1191 958 578 416 399 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 4145 1239 Size: 1013 1893 2579 Fragments arranged by size: 2579 1893 1013 Bpu10I CC'TnA_GC Cuts at: 2265 2563 2265 Size: 298 5187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2068 2606 3610 4475 4773 2068 Size: 538 1004 865 298 2780 Fragments arranged by size: 2780 1004 865 538 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2210 4148 882 Size: 617 711 1938 2219 Fragments arranged by size: 2219 1938 711 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 493 Size: 711 2072 2702 Fragments arranged by size: 2702 2072 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2574 3292 3674 4309 Size: 53 83 186 936 1041 718 382 635 Cuts at: 4309 275 Size: 1451 Fragments arranged by size: 1451 1041 936 718 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3918 4316 4590 4737 5148 3918 Size: 398 274 147 411 4255 Fragments arranged by size: 4255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2150 2180 1178 Size: 30 165 30 747 30 4483 Fragments arranged by size: 4483 747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2100 2249 2609 1186 Size: 914 149 360 4062 Fragments arranged by size: 4062 914 360 149 BseYI C'CCAG_C Cuts at: 2090 2392 2698 4688 5184 2090 Size: 302 306 1990 496 2391 Fragments arranged by size: 2391 1990 496 306 302 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4912 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2935 3696 4724 1540 Size: 275 186 934 761 1028 2301 Fragments arranged by size: 2301 1028 934 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2448 2598 2679 3546 3631 4702 2448 Size: 150 81 867 85 1071 3231 Fragments arranged by size: 3231 1071 867 150 85 81 BsmI GAATG_Cn' Cuts at: 2996 3089 2996 Size: 93 5392 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2448 2562 2598 2679 2686 2726 Size: 399 266 392 114 36 81 7 40 Cuts at: 2726 2773 2781 2804 3546 3631 4702 1391 Size: 47 8 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 392 266 114 85 81 47 40 36 23 8 7 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 3374 5104 3374 Size: 1730 3755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2840 844 Size: 1996 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2617 3372 1394 Size: 429 794 755 3507 Fragments arranged by size: 3507 794 755 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2283 2473 3980 4154 65 Size: 1183 1035 190 1507 174 1396 Fragments arranged by size: 1507 1396 1183 1035 190 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4129 4316 1700 Size: 110 2319 187 2869 Fragments arranged by size: 2869 2319 187 110 BsrGI T'GTAC_A Cuts at: 96 2134 96 Size: 2038 3447 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5485 BssSI C'ACGA_G Cuts at: 3545 4557 3545 Size: 1012 4473 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5485 BstBI TT'CG_AA Cuts at: 4289 4289 Size: 5485 BstEII G'GTnAC_C Cuts at: 1184 2362 1184 Size: 1178 4307 BstYI r'GATC_y Cuts at: 1972 2200 2815 3182 3567 3584 5036 5122 5134 Size: 228 615 367 385 17 1452 86 12 Cuts at: 5134 5480 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 615 385 367 346 228 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2358 2358 Size: 5485 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4933 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4746 Fragments arranged by size: 4746 683 56 BtsI GCAGTG_nn' Cuts at: 3001 3795 3815 3001 Size: 794 20 4671 Fragments arranged by size: 4671 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5450 DraI TTT'AAA Cuts at: 2789 2789 Size: 5485 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4492 809 Size: 507 3176 1802 Fragments arranged by size: 3176 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2242 2755 2932 Size: 54 1425 50 275 18 412 513 177 Cuts at: 2932 3821 5450 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 513 412 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2932 1537 Size: 275 1120 4090 Fragments arranged by size: 4090 1120 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2510 3415 1353 Size: 1157 905 3423 Fragments arranged by size: 3423 1157 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2120 4072 4446 4592 387 Size: 1064 138 531 1952 374 146 1280 Fragments arranged by size: 1952 1280 1064 531 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2252 3548 4145 4932 5200 1239 Size: 393 402 218 1296 597 787 268 1524 Fragments arranged by size: 1524 1296 787 597 402 393 268 218 EcoO109I rG'GnC_Cy Cuts at: 2777 2778 2777 Size: 1 5484 EcoRI G'AATT_C Cuts at: 2794 3172 2794 Size: 378 5107 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 4632 1678 Size: 2954 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2028 2060 2099 2131 4126 Size: 32 135 32 456 32 39 32 1995 Cuts at: 4126 4158 4200 4232 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1995 456 135 42 39 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2118 2829 3075 669 Size: 988 281 180 711 246 3079 Fragments arranged by size: 3079 988 711 281 246 180 HindIII A'AGCT_T Cuts at: 748 748 Size: 5485 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 3084 3084 Size: 5485 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2352 4599 4783 1305 Size: 1047 2247 184 2007 Fragments arranged by size: 2247 2007 1047 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2757 10 Size: 54 1425 343 925 2738 Fragments arranged by size: 2738 1425 925 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2461 2717 3443 3802 3961 518 Size: 648 1295 256 726 359 159 2042 Fragments arranged by size: 2042 1295 726 648 359 256 159 MspA1I CmG'CkG Cuts at: 1606 2901 3578 4726 4971 1606 Size: 1295 677 1148 245 2120 Fragments arranged by size: 2120 1295 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4933 NdeI CA'TA_TG Cuts at: 387 387 Size: 5485 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2843 4388 1132 Size: 97 1614 1545 2229 Fragments arranged by size: 2229 1614 1545 97 PasI CC'CwG_GG Cuts at: 1348 2686 2773 1348 Size: 1338 87 4060 Fragments arranged by size: 4060 1338 87 PciI A'CATG_T Cuts at: 4384 4384 Size: 5485 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5485 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 5067 5099 3554 Size: 32 1481 32 3940 Fragments arranged by size: 3940 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 3055 3055 Size: 5485 PspOMI G'GGCC_C Cuts at: 2769 2777 2769 Size: 8 5477 PstI C_TGCA'G Cuts at: 830 2588 2837 830 Size: 1758 249 3478 Fragments arranged by size: 3478 1758 249 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SbfI CC_TGCA'GG Cuts at: 2837 2837 Size: 5485 ScaI AGT'ACT Cuts at: 3733 5445 3733 Size: 1712 3773 SfcI C'TryA_G Cuts at: 826 1045 2584 2833 3968 4649 4840 826 Size: 219 1539 249 1135 681 191 1471 Fragments arranged by size: 1539 1471 1135 681 249 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2768 2812 1872 Size: 896 44 4545 Fragments arranged by size: 4545 896 44 SmlI C'TyrA_G Cuts at: 820 1017 1943 2047 2621 3589 4490 4752 820 Size: 197 926 104 574 968 901 262 1553 Fragments arranged by size: 1553 968 926 901 574 262 197 104 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 3409 5 Size: 47 3357 2081 Fragments arranged by size: 3357 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5485 StyI C'CwwG_G Cuts at: 513 1065 2061 2376 2924 513 Size: 552 996 315 548 3074 Fragments arranged by size: 3074 996 552 548 315 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3512 3529 3682 3867 4312 325 Size: 1255 1932 17 153 185 445 1498 Fragments arranged by size: 1932 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2134 3731 5443 Size: 274 80 33 51 1443 157 1597 1712 Cuts at: 5443 96 Size: 138 Fragments arranged by size: 1712 1597 1443 274 157 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5445 5477 5445 Size: 32 5453 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2821 2821 Size: 5485 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2383 2383 Size: 5485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5485 XmaI C'CCGG_G Cuts at: 1870 2766 2810 1870 Size: 896 44 4545 Fragments arranged by size: 4545 896 44 XmnI GAAnn'nnTTC Cuts at: 3614 3614 Size: 5485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2575 3293 276 Size: 53 83 186 936 1041 718 2468 Fragments arranged by size: 2468 1041 936 718 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI