################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeIII HindIII HinfI HpaI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MscI MspA1I MunI MvnI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 161 TCCGGA T^CCGGA AcsI 229 AAATTT [GA]^AATT[CT] AflIII 554 ACATGT A^C[GA][CT]GT AlwI 177 GGATC GGATC(4/5) AlwNI 361 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 229 AAATTT [GA]^AATT[CT] BanI 695 GGCGCC G^G[CT][GA]CC BbeI 695 GGCGCC GGCGC^C BcnI 638 CCCGG CC^[GC]GG BsaI 525 GGTCTC GGTCTC(1/5) BsaWI 161 TCCGGA [AT]^CCGG[AT] BseAI 161 TCCGGA T^CCGGA BsgI 331 GTGCAG GTGCAG(16/14) BsiYI 686 CCAGTGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 686 CCAGTGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspEI 161 TCCGGA T^CCGGA BspLU11I 554 ACATGT A^CATGT BstYI 177 GGATCT [GA]^GATC[CT] DsaI 5 CCATGG C^C[GA][CT]GG EarI 751 CTCTTC CTCTTC(1/4) EcoT14I 5 CCATGG C^C[AT][AT]GG HaeII 695 GGCGCC [GA]GCGC^[CT] HgaI 423 GCGTC GACGC(5/10) HphI 27 GGTGA GGTGA(8/7) KasI 695 GGCGCC G^GCGCC Ksp632I 751 CTCTTC CTCTTC(1/4) MflI 177 GGATCT [GA]^GATC[CT] MroI 161 TCCGGA T^CCGGA MseI 233 TTAA T^TAA MslI 537 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 695 GGCGCC GG^CGCC NciI 638 CCCGG CC^[GC]GG NcoI 5 CCATGG C^CATGG NdeI 679 CATATG CA^TATG NspI 554 ACATGT [GA]CATG^[CT] ScaI 280 AGTACT AGT^ACT SfoI 695 GGCGCC GGC^GCC StyI 5 CCATGG C^C[AT][AT]GG TaqI 128 TCGA T^CGA Tru9I 233 TTAA T^TAA TthHB8I 128 TCGA T^CGA XbaI 180 TCTAGA T^CTAGA XhoII 177 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 422 GGCGTC G[GA]^CG[CT]C 695 GGCGCC AfaI 40 GTAC GT^AC 281 GTAC BanII 88 GAGCTC G[GA]GC[CT]^C 436 GAGCTC BfaI 181 CTAG C^TAG 224 CTAG BsaHI 422 GGCGTC G[GA]^CG[CT]C 695 GGCGCC BseRI 91 CTCCTC GAGGAG(10/8) 466 GAGGAG BsmAI 374 GAGAC GTCTC(1/5) 526 GTCTC BstNI 63 CCAGG CC^[AT]GG 96 CCAGG Cac8I 333 GCAGGC GC[ACGT]^[ACGT]GC 474 GCAGGC CfoI 564 GCGC GCG^C 696 GCGC EcoRII 63 CCAGG ^CC[AT]GG 96 CCAGG HapII 162 CCGG C^CGG 639 CCGG HhaI 564 GCGC GCG^C 696 GCGC Hin1I 422 GGCGTC G[GA]^CG[CT]C 695 GGCGCC HinP1I 564 GCGC G^CGC 696 GCGC HincII 52 GTCAAC GT[CT]^[GA]AC 318 GTCAAC HindII 52 GTCAAC GT[CT]^[GA]AC 318 GTCAAC HpaII 162 CCGG C^CGG 639 CCGG MaeI 181 CTAG C^TAG 224 CTAG MspI 162 CCGG C^CGG 639 CCGG MvaI 63 CCAGG CC^[AT]GG 96 CCAGG RsaI 40 GTAC GT^AC 281 GTAC SacI 88 GAGCTC GAGCT^C 436 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AluI AspHI AvaII BbvI BmyI BpmI BsaJI BsiHKAI BsmFI Bsp1286I BsrI BssKI BstF5I Cfr13I DdeI DpnII DraII EcoO109I Fnu4HI FokI ItaI MboI MboII MnlI MwoI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I TspRI