################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BbrPI BbsI BcgI BciVI BclI BfrI BlnI BpmI BpuAI BsaAI BsaWI BseAI BseRI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco52I EcoO65I EcoRV FbaI FseI FspI KpnI Ksp632I KspI MluI MluNI MroI MscI MvnI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcyI 130 GGCGCC G[GA]^CG[CT]C AflIII 1533 ACACGT A^C[GA][CT]GT AocI 1773 CCTGAGG CC^T[ACGT]AGG Aor51HI 1982 AGCGCT AGC^GCT AspHI 328 GAGCTC G[AT]GC[AT]^C BbeI 130 GGCGCC GGCGC^C BbvCI 1567 GCTGAGG CCTCAGC(-5/-2) BglII 867 AGATCT A^GATCT BlpI 855 GCTCAGC GC^T[ACGT]AGC Bpu1102I 855 GCTCAGC GC^T[ACGT]AGC BsaBI 469 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 130 GGCGCC G[GA]^CG[CT]C BsgI 1097 GTGCAG GTGCAG(16/14) BsiEI 1919 CGGTCG CG[GA][CT]^CG BsiHKAI 328 GAGCTC G[AT]GC[AT]^C BspHI 1269 TCATGA T^CATGA BsrBI 1916 GAGCGG CCGCTC(-3/-3) BssSI 1873 CTCGTG CACGAG(-5/-1) BstAPI 1503 GCATTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2113 TTCGAA TT^CGAA BstXI 2027 CCATGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1773 CCTGAGG CC^T[ACGT]AGG CelII 855 GCTCAGC GC^T[ACGT]AGC DraIII 1870 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 353 TGGCCG [CT]^GGCC[GA] Eco47III 1982 AGCGCT AGC^GCT Eco81I 1773 CCTGAGG CC^T[ACGT]AGG EcoNI 1219 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1779 GAATTC G^AATTC HgaI 8 GCGTC GACGC(5/10) Hin1I 130 GGCGCC G[GA]^CG[CT]C HincII 1618 GTTAAC GT[CT]^[GA]AC HindII 1618 GTTAAC GT[CT]^[GA]AC HindIII 419 AAGCTT A^AGCTT HpaI 1618 GTTAAC GTT^AAC KasI 130 GGCGCC G^GCGCC MamI 469 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 832 CAATTG C^AATTG MunI 832 CAATTG C^AATTG NarI 130 GGCGCC GG^CGCC NdeI 1832 CATATG CA^TATG NspV 2113 TTCGAA TT^CGAA PpuMI 1067 GGGACCC [GA]G^G[AT]CC[CT] PstI 1706 CTGCAG CTGCA^G RcaI 1269 TCATGA T^CATGA SacI 328 GAGCTC GAGCT^C ScaI 1592 AGTACT AGT^ACT SexAI 1546 ACCAGGT A^CC[AT]GGT SfoI 130 GGCGCC GGC^GCC SfuI 2113 TTCGAA TT^CGAA SphI 1153 GCATGC GCATG^C StuI 1164 AGGCCT AGG^CCT Tsp45I 1537 GTCAC ^GT[GC]AC XbaI 588 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 941 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 1651 CAGCCACTG ApaI 95 GGGCCC GGGCC^C 301 GGGCCC Asp700I 223 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 706 GAACACATTC BamHI 89 GGATCC G^GATCC 1956 GGATCC BglI 59 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 355 GCCGACTTGGC BsaI 119 GGTCTC GGTCTC(1/5) 757 GGTCTC BsmI 397 GCATTC GAATGC(1/-1) 1503 GCATTC BstF5I 1768 CATCC GGATG(2/0) 1022 GGATG DraII 301 GGGCCCC [GA]G^G[ACGT]CC[CT] 1067 GGGACCC EcoO109I 301 GGGCCCC [GA]G^G[ACGT]CC[CT] 1067 GGGACCC EcoT22I 1155 ATGCAT ATGCA^T 1501 ATGCAT FauI 56 CCCGC CCCGC(4/6) 64 GCGGG FokI 1768 CATCC GGATG(9/13) 1022 GGATG HphI 384 TCACC GGTGA(8/7) 772 TCACC MslI 459 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2028 CATGCTCATG NsiI 1155 ATGCAT ATGCA^T 1501 ATGCAT SspI 1111 AATATT AAT^ATT 1171 AATATT XcmI 1530 CCAACACGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1840 CCAAATACTGCCTGG XmnI 223 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 706 GAACACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BanI BanII BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I Van91I XhoII