(Circular) (Six-base) MAPSORT of: pFN21AE6709.seq Check: 6432 from: 1 to: 6844 pFN21AE6709 6844 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4654 278 Size: 53 83 186 936 3118 2468 Fragments arranged by size: 3118 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4187 1937 Size: 2250 4594 Acc65I G'GTAC_C Cuts at: 4165 4165 Size: 6844 AclI AA'CG_TT Cuts at: 1563 4971 5344 1563 Size: 3408 373 3063 Fragments arranged by size: 3408 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3303 3713 4040 4161 4907 6291 6559 1632 Size: 1671 410 327 121 746 1384 268 1917 Fragments arranged by size: 1917 1671 1384 746 410 327 268 121 AfeI AGC'GCT Cuts at: 3989 3989 Size: 6844 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6647 AflIII A'CryG_T Cuts at: 1203 3538 5743 6720 1203 Size: 2335 2205 977 1327 Fragments arranged by size: 2335 2205 1327 977 AgeI A'CCGG_T Cuts at: 5675 5675 Size: 6844 AhdI GACnn_n'nnGTC Cuts at: 5573 5573 Size: 6844 AleI CACnn'nnGTG Cuts at: 1166 2468 1166 Size: 1302 5542 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4019 1184 Size: 2835 4009 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2662 2694 2662 Size: 32 6812 AlwNI CAG_nnn'CTG Cuts at: 2951 3661 4587 6159 2951 Size: 710 926 1572 3636 Fragments arranged by size: 3636 1572 926 710 ApaI G_GGCC'C Cuts at: 2104 2310 2104 Size: 206 6638 ApaLI G'TGCA_C Cuts at: 4901 6057 4901 Size: 1156 5688 ApoI r'AATT_y Cuts at: 1493 2811 3052 3784 3821 4153 4379 4531 6640 Size: 1318 241 732 37 332 226 152 2109 Cuts at: 6640 1493 Size: 1697 Fragments arranged by size: 2109 1697 1318 732 332 241 226 152 37 AseI AT'TA_AT Cuts at: 160 1032 5398 160 Size: 872 4366 1606 Fragments arranged by size: 4366 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6844 AvaI C'yCGr_G Cuts at: 1870 1943 4169 1870 Size: 73 2226 4545 Fragments arranged by size: 4545 2226 73 BamHI G'GATC_C Cuts at: 2094 3961 4174 4541 2094 Size: 1867 213 367 4397 Fragments arranged by size: 4397 1867 367 213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2135 2441 3555 4165 5620 Size: 325 217 627 348 306 1114 610 1455 Cuts at: 5620 618 Size: 1842 Fragments arranged by size: 1842 1455 1114 627 610 348 325 306 217 BanII G_rGCy'C Cuts at: 1391 2104 2310 2337 2920 3126 4032 4163 1391 Size: 713 206 27 583 206 906 131 4072 Fragments arranged by size: 4072 906 713 583 206 206 131 27 BbeI G_GCGC'C Cuts at: 2139 2139 Size: 6844 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5873 BbvCI CC'TCA_GC Cuts at: 3573 3573 Size: 6844 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5035 5069 1903 Size: 34 50 34 3014 34 3678 Fragments arranged by size: 3678 3014 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4736 5946 4736 Size: 1210 5634 BclI T'GATC_A Cuts at: 1548 1548 Size: 6844 BfrBI ATG'CAT Cuts at: 3162 3508 3162 Size: 346 6498 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2070 2366 5455 Size: 107 122 71 631 605 398 296 3089 Cuts at: 5455 136 Size: 1525 Fragments arranged by size: 3089 1525 631 605 398 296 122 107 71 BglII A'GATC_T Cuts at: 2872 6839 2872 Size: 3967 2877 BlpI GC'TnA_GC Cuts at: 2861 4261 2861 Size: 1400 5444 Bme1580I G_kGCm'C Cuts at: 1790 2104 2224 2310 3560 4093 4905 6061 1790 Size: 314 120 86 1250 533 812 1156 2573 Fragments arranged by size: 2573 1250 1156 812 533 314 120 86 BmgBI CAC'GTC Cuts at: 1318 3541 1318 Size: 2223 4621 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3395 3555 4575 Size: 416 53 83 399 24 1978 160 1020 Cuts at: 4575 5533 442 Size: 958 1753 Fragments arranged by size: 1978 1753 1020 958 416 399 160 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6844 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5504 1239 Size: 4265 2579 Bpu10I CC'TnA_GC Cuts at: 2211 2394 3573 2211 Size: 183 1179 5482 Fragments arranged by size: 5482 1179 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2278 3421 3788 4969 5834 6132 2278 Size: 1143 367 1181 865 298 2990 Fragments arranged by size: 2990 1181 1143 865 367 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2130 2768 5507 882 Size: 617 631 638 2739 2219 Fragments arranged by size: 2739 2219 638 631 617 BsaAI yAC'GTr Cuts at: 493 1204 4635 493 Size: 711 3431 2702 Fragments arranged by size: 3431 2702 711 BsaBI GATnn'nnATC Cuts at: 2478 6838 2478 Size: 4360 2484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 4651 5033 5668 Size: 53 83 186 936 603 2515 382 635 Cuts at: 5668 275 Size: 1451 Fragments arranged by size: 2515 1451 936 635 603 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5277 5675 5949 6096 6507 5277 Size: 398 274 147 411 5614 Fragments arranged by size: 5614 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6619 Fragments arranged by size: 6619 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6844 BseYI C'CCAG_C Cuts at: 2023 2738 3125 6047 6543 2023 Size: 715 387 2922 496 2324 Fragments arranged by size: 2922 2324 715 496 387 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3123 1163 Size: 573 1387 4884 Fragments arranged by size: 4884 1387 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3927 4294 5055 6083 1540 Size: 275 186 1926 367 761 1028 2301 Fragments arranged by size: 2301 1926 1028 761 367 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 4905 4990 6061 2337 Size: 2568 85 1071 3120 Fragments arranged by size: 3120 2568 1071 85 BsmI GAATG_Cn' Cuts at: 2402 3508 4355 4448 2402 Size: 1106 847 93 4798 Fragments arranged by size: 4798 1106 847 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2224 2310 2337 2920 3126 3560 Size: 399 314 120 86 27 583 206 434 Cuts at: 3560 4032 4093 4163 4905 4990 6061 1391 Size: 472 61 70 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 583 472 434 399 314 206 120 86 85 70 61 27 BspEI T'CCGG_A Cuts at: 6507 6507 Size: 6844 BspHI T'CATG_A Cuts at: 3274 4733 6463 3274 Size: 1459 1730 3655 Fragments arranged by size: 3655 1730 1459 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4199 844 Size: 3355 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3923 4731 1394 Size: 429 2100 808 3507 Fragments arranged by size: 3507 2100 808 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5339 5513 65 Size: 1183 4091 174 1396 Fragments arranged by size: 4091 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5488 5675 1700 Size: 110 3678 187 2869 Fragments arranged by size: 3678 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6844 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6844 BssSI C'ACGA_G Cuts at: 3878 4904 5916 3878 Size: 1026 1012 4806 Fragments arranged by size: 4806 1026 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3514 794 Size: 2720 4124 BstBI TT'CG_AA Cuts at: 4119 5648 4119 Size: 1529 5315 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6844 BstXI CCAn_nnnn'nTGG Cuts at: 4039 4039 Size: 6844 BstYI r'GATC_y Cuts at: 1972 2094 2872 3961 4108 4174 4541 4926 4943 Size: 122 778 1089 147 66 367 385 17 Cuts at: 4943 6395 6481 6493 6839 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1089 778 385 367 346 147 122 86 66 17 12 Bsu36I CC'TnA_GG Cuts at: 3779 3779 Size: 6844 BtgI C'CryG_G Cuts at: 513 1065 2130 2445 3361 3763 3933 4012 513 Size: 552 1065 315 916 402 170 79 3345 Fragments arranged by size: 3345 1065 916 552 402 315 170 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6105 Fragments arranged by size: 6105 683 56 BtsI GCAGTG_nn' Cuts at: 2625 3659 4360 5154 5174 2625 Size: 1034 701 794 20 4295 Fragments arranged by size: 4295 1034 794 701 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6844 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2529 2564 2871 2906 529 Size: 35 1965 35 307 35 4467 Fragments arranged by size: 4467 1965 307 35 35 35 DraI TTT'AAA Cuts at: 4148 4148 Size: 6844 DraIII CAC_nnn'GTG Cuts at: 3880 3880 Size: 6844 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5851 809 Size: 507 4535 1802 Fragments arranged by size: 4535 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 4291 5180 Size: 54 1425 50 275 18 528 1933 889 Cuts at: 5180 6809 8 Size: 1629 43 Fragments arranged by size: 1933 1629 1425 889 528 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4291 1537 Size: 275 2479 4090 Fragments arranged by size: 4090 2479 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4774 1353 Size: 3421 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5431 5805 5951 387 Size: 1064 138 3842 374 146 1280 Fragments arranged by size: 3842 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2335 2335 Size: 6844 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3303 3713 4040 4161 4907 5504 6291 Size: 393 1671 410 327 121 746 597 787 Cuts at: 6291 6559 1239 Size: 268 1524 Fragments arranged by size: 1671 1524 787 746 597 410 393 327 268 121 EcoNI CCTnn'n_nnAGG Cuts at: 3228 3228 Size: 6844 EcoO109I rG'GnC_Cy Cuts at: 2307 3073 2307 Size: 766 6078 EcoRI G'AATT_C Cuts at: 3784 4153 4531 3784 Size: 369 378 6097 Fragments arranged by size: 6097 378 369 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2719 4070 2719 Size: 1351 5493 FspI TGC'GCA Cuts at: 5350 5350 Size: 6844 HaeII r_GCGC'y Cuts at: 1678 2139 2191 3991 5991 1678 Size: 461 52 1800 2000 2531 Fragments arranged by size: 2531 2000 1800 461 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 3463 3495 5485 Size: 32 135 32 749 32 1110 32 1990 Cuts at: 5485 5517 5559 5591 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1990 1110 749 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3625 4188 4434 669 Size: 988 281 1687 563 246 3079 Fragments arranged by size: 3079 1687 988 563 281 246 HindIII A'AGCT_T Cuts at: 748 2424 748 Size: 1676 5168 HpaI GTT'AAC Cuts at: 3625 4434 3625 Size: 809 6035 KasI G'GCGC_C Cuts at: 2135 2135 Size: 6844 KpnI G_GTAC'C Cuts at: 4169 4169 Size: 6844 MfeI C'AATT_G Cuts at: 2837 4443 2837 Size: 1606 5238 MluI A'CGCG_T Cuts at: 6720 6720 Size: 6844 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1996 3473 4125 5958 6142 1305 Size: 691 1477 652 1833 184 2007 Fragments arranged by size: 2007 1833 1477 691 652 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5022 Fragments arranged by size: 5022 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 4037 4802 5161 5320 518 Size: 648 1302 1569 765 359 159 2042 Fragments arranged by size: 2042 1569 1302 765 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2121 3716 3862 4260 4937 6085 6330 1606 Size: 515 1595 146 398 677 1148 245 2120 Fragments arranged by size: 2120 1595 1148 677 515 398 245 146 NarI GG'CG_CC Cuts at: 2136 2136 Size: 6844 NcoI C'CATG_G Cuts at: 513 1065 2445 3763 3933 513 Size: 552 1380 1318 170 3424 Fragments arranged by size: 3424 1380 1318 552 170 NdeI CA'TA_TG Cuts at: 387 3838 387 Size: 3451 3393 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6844 NotI GC'GGCC_GC Cuts at: 4291 4291 Size: 6844 NsiI A_TGCA'T Cuts at: 3164 3510 3164 Size: 346 6498 NspI r_CATG'y Cuts at: 1132 1229 2292 3162 3503 4202 5747 1132 Size: 97 1063 870 341 699 1545 2229 Fragments arranged by size: 2229 1545 1063 870 699 341 97 PasI CC'CwG_GG Cuts at: 1348 2158 3069 1348 Size: 810 911 5123 Fragments arranged by size: 5123 911 810 PciI A'CATG_T Cuts at: 5743 5743 Size: 6844 PflMI CCAn_nnn'nTGG Cuts at: 1108 3493 3880 3939 1108 Size: 2385 387 59 4013 Fragments arranged by size: 4013 2385 387 59 PmeI GTTT'AAAC Cuts at: 4148 4148 Size: 6844 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4913 4945 6426 6458 4913 Size: 32 1481 32 5299 Fragments arranged by size: 5299 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3073 3073 Size: 6844 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6844 PsiI TTA'TAA Cuts at: 2808 4414 2808 Size: 1606 5238 PspOMI G'GGCC_C Cuts at: 2100 2306 2100 Size: 206 6638 PstI C_TGCA'G Cuts at: 830 3715 4196 830 Size: 2885 481 3478 Fragments arranged by size: 3478 2885 481 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6844 PvuII CAG'CTG Cuts at: 2121 3716 3862 2121 Size: 1595 146 5103 Fragments arranged by size: 5103 1595 146 SacI G_AGCT'C Cuts at: 2337 2337 Size: 6844 SalI G'TCGA_C Cuts at: 1936 4186 1936 Size: 2250 4594 SanDI GG'GwC_CC Cuts at: 3073 3073 Size: 6844 SbfI CC_TGCA'GG Cuts at: 4196 4196 Size: 6844 ScaI AGT'ACT Cuts at: 3599 5092 6804 3599 Size: 1493 1712 3639 Fragments arranged by size: 3639 1712 1493 SexAI A'CCwGG_T Cuts at: 3551 3551 Size: 6844 SfcI C'TryA_G Cuts at: 826 1045 3085 3711 3956 4192 5327 6008 6199 Size: 219 2040 626 245 236 1135 681 191 Cuts at: 6199 826 Size: 1471 Fragments arranged by size: 2040 1471 1135 681 626 245 236 219 191 SfoI GGC'GCC Cuts at: 2137 2137 Size: 6844 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6844 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6844 SmaI CCC'GGG Cuts at: 1872 4171 1872 Size: 2299 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2257 3436 3803 4948 5849 6111 Size: 197 926 314 1179 367 1145 901 262 Cuts at: 6111 820 Size: 1553 Fragments arranged by size: 1553 1179 1145 926 901 367 314 262 197 SnaBI TAC'GTA Cuts at: 493 4635 493 Size: 4142 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6844 SphI G_CATG'C Cuts at: 1132 3162 4202 1132 Size: 2030 1040 3774 Fragments arranged by size: 3774 2030 1040 SspI AAT'ATT Cuts at: 5 52 3118 3178 4768 5 Size: 47 3066 60 1590 2081 Fragments arranged by size: 3066 2081 1590 60 47 StuI AGG'CCT Cuts at: 1517 3171 1517 Size: 1654 5190 StyI C'CwwG_G Cuts at: 513 1065 2445 2923 3763 3933 4283 513 Size: 552 1380 478 840 170 350 3074 Fragments arranged by size: 3074 1380 840 552 478 350 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4871 4888 5041 5226 5671 325 Size: 1255 3291 17 153 185 445 1498 Fragments arranged by size: 3291 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2654 3597 5090 Size: 274 80 33 51 1443 677 943 1493 Cuts at: 5090 6802 96 Size: 1712 138 Fragments arranged by size: 1712 1493 1443 943 677 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6804 6836 6804 Size: 32 6812 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6844 XbaI T'CTAG_A Cuts at: 2593 4180 2593 Size: 1587 5257 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3542 3852 3542 Size: 310 6534 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6844 XmaI C'CCGG_G Cuts at: 1870 4169 1870 Size: 2299 4545 XmnI GAAnn'nnTTC Cuts at: 2232 2715 4973 2232 Size: 483 2258 4103 Fragments arranged by size: 4103 2258 483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4652 276 Size: 53 83 186 936 3118 2468 Fragments arranged by size: 3118 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI