(Linear) (Six-base) MAPSORT of: KIEE6709.seq Check: 7845 from: 1 to: 2139 KIEE6709.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1298 1708 2035 2139 Size: 1298 410 327 104 Fragments arranged by size: 1298 410 327 104 AfeI AGC'GCT Cuts at: 0 1984 2139 Size: 1984 155 AflIII A'CryG_T Cuts at: 0 1533 2139 Size: 1533 606 AleI CACnn'nnGTG Cuts at: 0 463 2139 Size: 463 1676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2014 2139 Size: 2014 125 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 657 689 2139 Size: 657 32 1450 Fragments arranged by size: 1450 657 32 AlwNI CAG_nnn'CTG Cuts at: 0 946 1656 2139 Size: 946 710 483 Fragments arranged by size: 946 710 483 ApaI G_GGCC'C Cuts at: 0 99 305 2139 Size: 99 206 1834 Fragments arranged by size: 1834 206 99 ApoI r'AATT_y Cuts at: 0 806 1047 1779 1816 2139 Size: 806 241 732 37 323 Fragments arranged by size: 806 732 323 241 37 BamHI G'GATC_C Cuts at: 0 89 1956 2139 Size: 89 1867 183 Fragments arranged by size: 1867 183 89 BanI G'GyrC_C Cuts at: 0 130 436 1550 2139 Size: 130 306 1114 589 Fragments arranged by size: 1114 589 306 130 BanII G_rGCy'C Cuts at: 0 99 305 332 915 1121 2027 2139 Size: 99 206 27 583 206 906 112 Fragments arranged by size: 906 583 206 206 112 99 27 BbeI G_GCGC'C Cuts at: 0 134 2139 Size: 134 2005 BbvCI CC'TCA_GC Cuts at: 0 1568 2139 Size: 1568 571 BfrBI ATG'CAT Cuts at: 0 1157 1503 2139 Size: 1157 346 636 Fragments arranged by size: 1157 636 346 BglI GCCn_nnn'nGGC Cuts at: 0 65 361 2139 Size: 65 296 1778 Fragments arranged by size: 1778 296 65 BglII A'GATC_T Cuts at: 0 867 2139 Size: 867 1272 BlpI GC'TnA_GC Cuts at: 0 856 2139 Size: 856 1283 Bme1580I G_kGCm'C Cuts at: 0 99 219 305 1555 2088 2139 Size: 99 120 86 1250 533 51 Fragments arranged by size: 1250 533 120 99 86 51 BmgBI CAC'GTC Cuts at: 0 1536 2139 Size: 1536 603 BmrI ACTGGGnnnn_n' Cuts at: 0 1390 1550 2139 Size: 1390 160 589 Fragments arranged by size: 1390 589 160 Bpu10I CC'TnA_GC Cuts at: 0 206 389 1568 2139 Size: 206 183 1179 571 Fragments arranged by size: 1179 571 206 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 273 1416 1783 2139 Size: 273 1143 367 356 Fragments arranged by size: 1143 367 356 273 BsaI GGTCTCn'nnnn_ Cuts at: 0 125 763 2139 Size: 125 638 1376 Fragments arranged by size: 1376 638 125 BsaBI GATnn'nnATC Cuts at: 0 473 2139 Size: 473 1666 BsaHI Gr'CG_yC Cuts at: 0 131 2139 Size: 131 2008 BseYI C'CCAG_C Cuts at: 0 18 733 1120 2139 Size: 18 715 387 1019 Fragments arranged by size: 1019 715 387 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1118 2139 Size: 1118 1021 BsiEI CG_ry'CG Cuts at: 0 1922 2139 Size: 1922 217 BsiHKAI G_wGCw'C Cuts at: 0 332 2139 Size: 332 1807 BsmI GAATG_Cn' Cuts at: 0 397 1503 2139 Size: 397 1106 636 Fragments arranged by size: 1106 636 397 Bsp1286I G_dGCh'C Cuts at: 0 99 219 305 332 915 1121 1555 2027 Size: 99 120 86 27 583 206 434 472 Cuts at: 2027 2088 2139 Size: 61 51 Fragments arranged by size: 583 472 434 206 120 99 86 61 51 27 BspHI T'CATG_A Cuts at: 0 1269 2139 Size: 1269 870 BsrBI CCG'CTC Cuts at: 0 1918 2139 Size: 1918 221 BssSI C'ACGA_G Cuts at: 0 1873 2139 Size: 1873 266 BstAPI GCAn_nnn'nTGC Cuts at: 0 1509 2139 Size: 1509 630 BstBI TT'CG_AA Cuts at: 0 2114 2139 Size: 2114 25 BstXI CCAn_nnnn'nTGG Cuts at: 0 2034 2139 Size: 2034 105 BstYI r'GATC_y Cuts at: 0 89 867 1956 2103 2139 Size: 89 778 1089 147 36 Fragments arranged by size: 1089 778 147 89 36 Bsu36I CC'TnA_GG Cuts at: 0 1774 2139 Size: 1774 365 BtgI C'CryG_G Cuts at: 0 125 440 1356 1758 1928 2007 2139 Size: 125 315 916 402 170 79 132 Fragments arranged by size: 916 402 315 170 132 125 79 BtsI GCAGTG_nn' Cuts at: 0 620 1654 2139 Size: 620 1034 485 Fragments arranged by size: 1034 620 485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 524 559 866 901 2139 Size: 524 35 307 35 1238 Fragments arranged by size: 1238 524 307 35 35 DraIII CAC_nnn'GTG Cuts at: 0 1875 2139 Size: 1875 264 EaeI y'GGCC_r Cuts at: 0 353 2139 Size: 353 1786 EcoICRI GAG'CTC Cuts at: 0 330 2139 Size: 330 1809 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1298 1708 2035 2139 Size: 1298 410 327 104 Fragments arranged by size: 1298 410 327 104 EcoNI CCTnn'n_nnAGG Cuts at: 0 1223 2139 Size: 1223 916 EcoO109I rG'GnC_Cy Cuts at: 0 302 1068 2139 Size: 302 766 1071 Fragments arranged by size: 1071 766 302 EcoRI G'AATT_C Cuts at: 0 1779 2139 Size: 1779 360 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 714 2065 2139 Size: 714 1351 74 Fragments arranged by size: 1351 714 74 HaeII r_GCGC'y Cuts at: 0 134 186 1986 2139 Size: 134 52 1800 153 Fragments arranged by size: 1800 153 134 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 316 348 1458 1490 2139 Size: 316 32 1110 32 649 Fragments arranged by size: 1110 649 316 32 32 HincII GTy'rAC Cuts at: 0 1620 2139 Size: 1620 519 HindIII A'AGCT_T Cuts at: 0 419 2139 Size: 419 1720 HpaI GTT'AAC Cuts at: 0 1620 2139 Size: 1620 519 KasI G'GCGC_C Cuts at: 0 130 2139 Size: 130 2009 MfeI C'AATT_G Cuts at: 0 832 2139 Size: 832 1307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1468 2120 2139 Size: 1468 652 19 Fragments arranged by size: 1468 652 19 MslI CAynn'nnrTG Cuts at: 0 463 2032 2139 Size: 463 1569 107 Fragments arranged by size: 1569 463 107 MspA1I CmG'CkG Cuts at: 0 116 1711 1857 2139 Size: 116 1595 146 282 Fragments arranged by size: 1595 282 146 116 NarI GG'CG_CC Cuts at: 0 131 2139 Size: 131 2008 NcoI C'CATG_G Cuts at: 0 440 1758 1928 2139 Size: 440 1318 170 211 Fragments arranged by size: 1318 440 211 170 NdeI CA'TA_TG Cuts at: 0 1833 2139 Size: 1833 306 NsiI A_TGCA'T Cuts at: 0 1159 1505 2139 Size: 1159 346 634 Fragments arranged by size: 1159 634 346 NspI r_CATG'y Cuts at: 0 287 1157 1498 2139 Size: 287 870 341 641 Fragments arranged by size: 870 641 341 287 PasI CC'CwG_GG Cuts at: 0 153 1064 2139 Size: 153 911 1075 Fragments arranged by size: 1075 911 153 PflMI CCAn_nnn'nTGG Cuts at: 0 1488 1875 1934 2139 Size: 1488 387 59 205 Fragments arranged by size: 1488 387 205 59 PpuMI rG'GwC_Cy Cuts at: 0 1068 2139 Size: 1068 1071 PsiI TTA'TAA Cuts at: 0 803 2139 Size: 803 1336 PspOMI G'GGCC_C Cuts at: 0 95 301 2139 Size: 95 206 1838 Fragments arranged by size: 1838 206 95 PstI C_TGCA'G Cuts at: 0 1710 2139 Size: 1710 429 PvuII CAG'CTG Cuts at: 0 116 1711 1857 2139 Size: 116 1595 146 282 Fragments arranged by size: 1595 282 146 116 SacI G_AGCT'C Cuts at: 0 332 2139 Size: 332 1807 SanDI GG'GwC_CC Cuts at: 0 1068 2139 Size: 1068 1071 ScaI AGT'ACT Cuts at: 0 1594 2139 Size: 1594 545 SexAI A'CCwGG_T Cuts at: 0 1546 2139 Size: 1546 593 SfcI C'TryA_G Cuts at: 0 1080 1706 1951 2139 Size: 1080 626 245 188 Fragments arranged by size: 1080 626 245 188 SfoI GGC'GCC Cuts at: 0 132 2139 Size: 132 2007 SmlI C'TyrA_G Cuts at: 0 252 1431 1798 2139 Size: 252 1179 367 341 Fragments arranged by size: 1179 367 341 252 SphI G_CATG'C Cuts at: 0 1157 2139 Size: 1157 982 SspI AAT'ATT Cuts at: 0 1113 1173 2139 Size: 1113 60 966 Fragments arranged by size: 1113 966 60 StuI AGG'CCT Cuts at: 0 1166 2139 Size: 1166 973 StyI C'CwwG_G Cuts at: 0 440 918 1758 1928 2139 Size: 440 478 840 170 211 Fragments arranged by size: 840 478 440 211 170 TatI w'GTAC_w Cuts at: 0 649 1592 2139 Size: 649 943 547 Fragments arranged by size: 943 649 547 XbaI T'CTAG_A Cuts at: 0 588 2139 Size: 588 1551 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1537 1847 2139 Size: 1537 310 292 Fragments arranged by size: 1537 310 292 XmnI GAAnn'nnTTC Cuts at: 0 227 710 2139 Size: 227 483 1429 Fragments arranged by size: 1429 483 227 Enzymes that do cut: AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI ApoI BamHI BanI BanII BbeI BbvCI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaI BsaBI BsaHI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MslI MspA1I NarI NcoI NdeI NsiI NspI PasI PflMI PpuMI PsiI PspOMI PstI PvuII SacI SanDI ScaI SexAI SfcI SfoI SmlI SphI SspI StuI StyI TatI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbsI BcgI BciVI BclI BmtI BplI BpmI BsaAI BsaWI BsaXI BseRI BsiWI BsmBI BspEI BspMI BsrDI BsrFI BsrGI BssHII BstEII BstZ17I BtgZI ClaI DraI DrdI EagI EarI EciI EcoRV FseI FspI FspAI KpnI MluI MscI NaeI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TaqII TstI Tth111I XhoI XmaI ZraI