################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfrI BlnI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 280 GTAGAC GT^[AC][GT]AC AcsI 727 AAATTC [GA]^AATT[CT] AflIII 509 ACGTGT A^C[GA][CT]GT AlwNI 910 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 727 AAATTC [GA]^AATT[CT] AspHI 1038 GTGCTC G[AT]GC[AT]^C BalI 893 TGGCCA TGG^CCA BamHI 480 GGATCC G^GATCC BbvCI 961 GCTGAGG CCTCAGC(-5/-2) BfaI 100 CTAG C^TAG BglI 1018 GCCAGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 503 AGATCT A^GATCT BlpI 157 GCTGAGC GC^T[ACGT]AGC Bpu1102I 157 GCTGAGC GC^T[ACGT]AGC BsaAI 508 TACGTG [CT]AC^GT[GA] BsaI 292 GAGACC GGTCTC(1/5) BsiHKAI 1038 GTGCTC G[AT]GC[AT]^C BsmAI 292 GAGAC GTCTC(1/5) BsmI 957 GAATGC GAATGC(1/-1) BstAPI 1031 GCATCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 157 GCTGAGC GC^T[ACGT]AGC DraIII 73 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 575 CCATGG C^C[GA][CT]GG EaeI 893 TGGCCA [CT]^GGCC[GA] EcoNI 685 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HindIII 590 AAGCTT A^AGCTT MaeI 100 CTAG C^TAG MaeII 509 ACGT A^CGT MluNI 893 TGGCCA TGG^CCA MscI 893 TGGCCA TGG^CCA NcoI 575 CCATGG C^CATGG NspI 150 GCATGC [GA]CATG^[CT] PflMI 72 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 599 GAGTC GAGTC(4/5) SexAI 684 ACCTGGT A^CC[AT]GGT SmaI 866 CCCGGG CCC^GGG SmlI 419 CTCAAG C^T[CT][GA]AG SphI 150 GCATGC GCATG^C StuI 670 AGGCCT AGG^CCT TaqI 821 TCGA T^CGA TfiI 438 GATTC G^A[AT]TC Tsp509I 728 AATT ^AATT TthHB8I 821 TCGA T^CGA Van91I 72 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 866 CCCGGG C^CCGGG XmaI 866 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AfaI 84 GTAC GT^AC 384 GTAC ApaI 4 GGGCCC GGGCC^C 1025 GGGCCC BanII 4 GGGCCC G[GA]GC[CT]^C 1025 GGGCCC BbsI 680 GAAGAC GAAGAC(2/6) 544 GTCTTC BpuAI 680 GAAGAC GAAGAC(2/6) 544 GTCTTC BsrBI 214 GAGCGG CCGCTC(-3/-3) 271 GAGCGG HinfI 438 GATTC G^A[ACGT]TC 599 GAGTC PpuMI 48 GGGACCC [GA]G^G[AT]CC[CT] 985 GGGACCC RsaI 84 GTAC GT^AC 384 GTAC XcmI 460 CCAGCTGGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 476 CCAAGGATCCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AluI AlwI AvaI AvaII BanI BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI XhoII