(Circular) (Six-base) MAPSORT of: pFN21AE2280.seq Check: 2995 from: 1 to: 5923 pFN21AE2280 5923 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2286 3266 1937 Size: 349 980 4594 Fragments arranged by size: 4594 980 349 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2468 3986 5370 5638 1632 Size: 836 1518 1384 268 1917 Fragments arranged by size: 1917 1518 1384 836 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 2514 4822 5799 1203 Size: 1311 2308 977 1327 Fragments arranged by size: 2308 1327 1311 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 2920 3666 5238 2920 Size: 746 1572 3605 Fragments arranged by size: 3605 1572 746 ApaI G_GGCC'C Cuts at: 2013 3034 2013 Size: 1021 4902 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2732 3232 3458 3610 5719 1493 Size: 1239 500 226 152 2109 1697 Fragments arranged by size: 2109 1697 1239 500 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2601 2871 3141 3248 1870 Size: 73 658 270 270 107 4545 Fragments arranged by size: 4545 658 270 270 107 73 BamHI G'GATC_C Cuts at: 2485 3253 3620 2485 Size: 768 367 4788 Fragments arranged by size: 4788 768 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2611 3062 3113 3244 4699 Size: 325 217 627 824 451 51 131 1455 Cuts at: 4699 618 Size: 1842 Fragments arranged by size: 1842 1455 824 627 451 325 217 131 51 BanII G_rGCy'C Cuts at: 1391 2013 3034 3242 1391 Size: 622 1021 208 4072 Fragments arranged by size: 4072 1021 622 208 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2542 2692 928 Size: 971 643 150 4159 Fragments arranged by size: 4159 971 643 150 BbvCI CC'TCA_GC Cuts at: 2967 2967 Size: 5923 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3029 4534 136 Size: 107 122 71 631 605 1357 1505 1525 Fragments arranged by size: 1525 1505 1357 631 605 122 107 71 BglII A'GATC_T Cuts at: 2508 5918 2508 Size: 3410 2513 BlpI GC'TnA_GC Cuts at: 2163 3340 2163 Size: 1177 4746 Bme1580I G_kGCm'C Cuts at: 1790 2013 2616 3034 3118 3984 5140 1790 Size: 223 603 418 84 866 1156 2573 Fragments arranged by size: 2573 1156 866 603 418 223 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2125 2501 3654 Size: 416 53 83 399 24 708 376 1153 Cuts at: 3654 4612 442 Size: 958 1753 Fragments arranged by size: 1753 1153 958 708 416 399 376 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2294 2315 2367 3215 4583 1239 Size: 983 72 21 52 848 1368 2579 Fragments arranged by size: 2579 1368 983 848 72 52 21 Bpu10I CC'TnA_GC Cuts at: 2967 2967 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2409 4048 4913 5211 2409 Size: 1639 865 298 3121 Fragments arranged by size: 3121 1639 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 4586 882 Size: 617 792 2295 2219 Fragments arranged by size: 2295 2219 792 617 BsaAI yAC'GTr Cuts at: 493 1204 2515 3714 493 Size: 711 1311 1199 2702 Fragments arranged by size: 2702 1311 1199 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 1178 Size: 30 165 30 834 30 4834 Fragments arranged by size: 4834 834 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 3227 3230 1186 Size: 829 1212 3 3879 Fragments arranged by size: 3879 1212 829 3 BseYI C'CCAG_C Cuts at: 2012 2308 2464 2776 5126 5622 2012 Size: 296 156 312 2350 496 2313 Fragments arranged by size: 2350 2313 496 312 296 156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5350 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3047 3984 4069 5140 3047 Size: 937 85 1071 3830 Fragments arranged by size: 3830 1071 937 85 BsmI GAATG_Cn' Cuts at: 2968 3434 3527 2968 Size: 466 93 5364 Fragments arranged by size: 5364 466 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2616 3034 3047 3118 3242 3984 Size: 399 223 603 418 13 71 124 742 Cuts at: 3984 4069 5140 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 418 399 223 124 85 71 13 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3278 844 Size: 2434 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2221 2278 3810 1394 Size: 429 398 57 1532 3507 Fragments arranged by size: 3507 1532 429 398 57 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 3042 794 Size: 2248 3675 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2485 2508 2908 3253 3620 4005 4022 5474 Size: 513 23 400 345 367 385 17 1452 Cuts at: 5474 5560 5572 5918 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 513 400 385 367 346 345 86 23 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2580 513 Size: 552 939 576 3856 Fragments arranged by size: 3856 939 576 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2195 2942 3439 4233 4253 2195 Size: 747 497 794 20 3865 Fragments arranged by size: 3865 794 747 497 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DraIII CAC_nnn'GTG Cuts at: 2083 2083 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2898 3370 4259 Size: 54 1425 50 275 18 1068 472 889 Cuts at: 4259 5888 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1068 889 472 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2294 2315 2367 2468 3215 3986 Size: 393 590 72 21 52 101 747 771 Cuts at: 3986 4583 5370 5638 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 771 747 597 590 393 268 101 72 52 21 EcoNI CCTnn'n_nnAGG Cuts at: 2694 2694 Size: 5923 EcoO109I rG'GnC_Cy Cuts at: 2009 2054 2351 2991 2009 Size: 45 297 640 4941 Fragments arranged by size: 4941 640 297 45 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 5070 1678 Size: 3392 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2237 2251 2269 2283 2470 Size: 32 135 32 665 14 18 14 187 Cuts at: 2470 2502 4564 4596 4638 4670 1373 Size: 32 2062 32 42 32 2626 Fragments arranged by size: 2626 2062 665 187 135 42 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 2595 748 Size: 1847 4076 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2900 10 Size: 54 1425 343 1068 3033 Fragments arranged by size: 3033 1425 1068 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3881 4240 4399 518 Size: 648 2715 359 159 2042 Fragments arranged by size: 2715 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2468 2641 2837 3060 3339 4016 5164 5409 Size: 862 173 196 223 279 677 1148 245 Cuts at: 5409 1606 Size: 2120 Fragments arranged by size: 2120 1148 862 677 279 245 223 196 173 NcoI C'CATG_G Cuts at: 513 1065 2004 2580 513 Size: 552 939 576 3856 Fragments arranged by size: 3856 939 576 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2159 3281 4826 1132 Size: 97 930 1122 1545 2229 Fragments arranged by size: 2229 1545 1122 930 97 PasI CC'CwG_GG Cuts at: 1348 2354 2622 1348 Size: 1006 268 4649 Fragments arranged by size: 4649 1006 268 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2083 1108 Size: 975 4948 PfoI T'CCnGG_A Cuts at: 2255 2680 2255 Size: 425 5498 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3138 3170 3992 4024 5505 5537 3138 Size: 32 822 32 1481 32 3524 Fragments arranged by size: 3524 1481 822 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2054 2991 2054 Size: 937 4986 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 2009 3030 2009 Size: 1021 4902 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2468 2641 2837 2468 Size: 173 196 5554 Fragments arranged by size: 5554 196 173 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SanDI GG'GwC_CC Cuts at: 2054 2991 2054 Size: 937 4986 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SexAI A'CCwGG_T Cuts at: 2689 2689 Size: 5923 SfcI C'TryA_G Cuts at: 826 1045 3271 4406 5087 5278 826 Size: 219 2226 1135 681 191 1471 Fragments arranged by size: 2226 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2873 3250 1872 Size: 1001 377 4545 Fragments arranged by size: 4545 1001 377 SmlI C'TyrA_G Cuts at: 820 1017 1943 2424 4027 4928 5190 820 Size: 197 926 481 1603 901 262 1553 Fragments arranged by size: 1603 1553 926 901 481 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 2159 3281 1132 Size: 1027 1122 3774 Fragments arranged by size: 3774 1122 1027 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 2677 1517 Size: 1160 4763 StyI C'CwwG_G Cuts at: 513 1065 2004 2062 2481 2555 2580 3362 513 Size: 552 939 58 419 74 25 782 3074 Fragments arranged by size: 3074 939 782 552 419 74 58 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3950 3967 4120 4305 4750 325 Size: 1255 2370 17 153 185 445 1498 Fragments arranged by size: 2370 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 4169 5881 Size: 274 80 33 51 1443 111 2081 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 2081 1712 1443 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2935 2967 5883 5915 2935 Size: 32 2916 32 2943 Fragments arranged by size: 2943 2916 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2472 2488 2472 Size: 16 5907 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 2871 3248 1870 Size: 1001 377 4545 Fragments arranged by size: 4545 1001 377 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI