(Linear) (Six-base) MAPSORT of: KIEE2280.seq Check: 2603 from: 1 to: 1218 KIEE2280.orf With 182 enzymes: SgfI * July 15, 2010 18:56 .. AccI GT'mk_AC Cuts at: 0 281 1218 Size: 281 937 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 463 1218 Size: 463 755 AflIII A'CryG_T Cuts at: 0 509 1218 Size: 509 709 AlwNI CAG_nnn'CTG Cuts at: 0 915 1218 Size: 915 303 ApaI G_GGCC'C Cuts at: 0 8 1029 1218 Size: 8 1021 189 Fragments arranged by size: 1021 189 8 ApoI r'AATT_y Cuts at: 0 727 1218 Size: 727 491 AvaI C'yCGr_G Cuts at: 0 596 866 1136 1218 Size: 596 270 270 82 Fragments arranged by size: 596 270 270 82 BamHI G'GATC_C Cuts at: 0 480 1218 Size: 480 738 BanI G'GyrC_C Cuts at: 0 606 1057 1108 1218 Size: 606 451 51 110 Fragments arranged by size: 606 451 110 51 BanII G_rGCy'C Cuts at: 0 8 1029 1218 Size: 8 1021 189 Fragments arranged by size: 1021 189 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 537 687 1218 Size: 537 150 531 Fragments arranged by size: 537 531 150 BbvCI CC'TCA_GC Cuts at: 0 962 1218 Size: 962 256 BglI GCCn_nnn'nGGC Cuts at: 0 1024 1218 Size: 1024 194 BglII A'GATC_T Cuts at: 0 503 1218 Size: 503 715 BlpI GC'TnA_GC Cuts at: 0 158 1218 Size: 158 1060 Bme1580I G_kGCm'C Cuts at: 0 8 611 1029 1113 1218 Size: 8 603 418 84 105 Fragments arranged by size: 603 418 105 84 8 BmrI ACTGGGnnnn_n' Cuts at: 0 120 496 1218 Size: 120 376 722 Fragments arranged by size: 722 376 120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 289 310 362 1210 1218 Size: 217 72 21 52 848 8 Fragments arranged by size: 848 217 72 52 21 8 Bpu10I CC'TnA_GC Cuts at: 0 962 1218 Size: 962 256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 404 1218 Size: 404 814 BsaI GGTCTCn'nnnn_ Cuts at: 0 286 1218 Size: 286 932 BsaAI yAC'GTr Cuts at: 0 510 1218 Size: 510 708 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 232 262 1218 Size: 232 30 956 Fragments arranged by size: 956 232 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 10 1218 Size: 10 1208 BseYI C'CCAG_C Cuts at: 0 7 303 459 771 1218 Size: 7 296 156 312 447 Fragments arranged by size: 447 312 296 156 7 BsiHKAI G_wGCw'C Cuts at: 0 1042 1218 Size: 1042 176 BsmI GAATG_Cn' Cuts at: 0 963 1218 Size: 963 255 Bsp1286I G_dGCh'C Cuts at: 0 8 611 1029 1042 1113 1218 Size: 8 603 418 13 71 105 Fragments arranged by size: 603 418 105 71 13 8 BsrBI CCG'CTC Cuts at: 0 216 273 1218 Size: 216 57 945 Fragments arranged by size: 945 216 57 BstAPI GCAn_nnn'nTGC Cuts at: 0 1037 1218 Size: 1037 181 BstYI r'GATC_y Cuts at: 0 480 503 903 1218 Size: 480 23 400 315 Fragments arranged by size: 480 400 315 23 BtgI C'CryG_G Cuts at: 0 575 1218 Size: 575 643 BtsI GCAGTG_nn' Cuts at: 0 190 937 1218 Size: 190 747 281 Fragments arranged by size: 747 281 190 DraIII CAC_nnn'GTG Cuts at: 0 78 1218 Size: 78 1140 EaeI y'GGCC_r Cuts at: 0 893 1218 Size: 893 325 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 289 310 362 463 1210 1218 Size: 217 72 21 52 101 747 8 Fragments arranged by size: 747 217 101 72 52 21 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 689 1218 Size: 689 529 EcoO109I rG'GnC_Cy Cuts at: 0 4 49 346 986 1218 Size: 4 45 297 640 232 Fragments arranged by size: 640 297 232 45 4 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 232 246 264 278 465 497 1218 Size: 232 14 18 14 187 32 721 Fragments arranged by size: 721 232 187 32 18 14 14 HindIII A'AGCT_T Cuts at: 0 590 1218 Size: 590 628 MscI TGG'CCA Cuts at: 0 895 1218 Size: 895 323 MspA1I CmG'CkG Cuts at: 0 463 636 832 1055 1218 Size: 463 173 196 223 163 Fragments arranged by size: 463 223 196 173 163 NcoI C'CATG_G Cuts at: 0 575 1218 Size: 575 643 NspI r_CATG'y Cuts at: 0 154 1218 Size: 154 1064 PasI CC'CwG_GG Cuts at: 0 349 617 1218 Size: 349 268 601 Fragments arranged by size: 601 349 268 PflMI CCAn_nnn'nTGG Cuts at: 0 78 1218 Size: 78 1140 PfoI T'CCnGG_A Cuts at: 0 250 675 1218 Size: 250 425 543 Fragments arranged by size: 543 425 250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1133 1165 1218 Size: 1133 32 53 Fragments arranged by size: 1133 53 32 PpuMI rG'GwC_Cy Cuts at: 0 49 986 1218 Size: 49 937 232 Fragments arranged by size: 937 232 49 PspOMI G'GGCC_C Cuts at: 0 4 1025 1218 Size: 4 1021 193 Fragments arranged by size: 1021 193 4 PvuII CAG'CTG Cuts at: 0 463 636 832 1218 Size: 463 173 196 386 Fragments arranged by size: 463 386 196 173 SanDI GG'GwC_CC Cuts at: 0 49 986 1218 Size: 49 937 232 Fragments arranged by size: 937 232 49 SexAI A'CCwGG_T Cuts at: 0 684 1218 Size: 684 534 SmaI CCC'GGG Cuts at: 0 868 1218 Size: 868 350 SmlI C'TyrA_G Cuts at: 0 419 1218 Size: 419 799 SphI G_CATG'C Cuts at: 0 154 1218 Size: 154 1064 StuI AGG'CCT Cuts at: 0 672 1218 Size: 672 546 StyI C'CwwG_G Cuts at: 0 57 476 550 575 1218 Size: 57 419 74 25 643 Fragments arranged by size: 643 419 74 57 25 TatI w'GTAC_w Cuts at: 0 83 1218 Size: 83 1135 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 930 962 1218 Size: 930 32 256 Fragments arranged by size: 930 256 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 467 483 1218 Size: 467 16 735 Fragments arranged by size: 735 467 16 XmaI C'CCGG_G Cuts at: 0 866 1218 Size: 866 352 Enzymes that do cut: AccI AcuI AflIII AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrBI BstAPI BstYI BtgI BtsI DraIII EaeI Eco57MI EcoNI EcoO109I Hin4I HindIII MscI MspA1I NcoI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PvuII SanDI SexAI SmaI SmlI SphI StuI StyI TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI