################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BpmI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TspRI Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AciI 606 GCGG CCGC(-3/-1) AcsI 68 AAATTC [GA]^AATT[CT] AhdI 622 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 145 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 68 AAATTC [GA]^AATT[CT] Asp700I 583 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 622 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 356 GAGCAC G[AT]GC[AT]^C AvaI 263 CTCGAG C^[CT]CG[GA]G BbsI 233 GAAGAC GAAGAC(2/6) BclI 647 TGATCA T^GATCA BfaI 565 CTAG C^TAG BglII 312 AGATCT A^GATCT BlpI 346 GCTAAGC GC^T[ACGT]AGC BmyI 356 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 346 GCTAAGC GC^T[ACGT]AGC BpuAI 233 GAAGAC GAAGAC(2/6) BsaI 391 GAGACC GGTCTC(1/5) BsaWI 6 ACCGGA [AT]^CCGG[AT] BsiHKAI 356 GAGCAC G[AT]GC[AT]^C BsmFI 227 GGGAC GGGAC(10/14) BsoBI 263 CTCGAG C^[CT]CG[GA]G Bsp1286I 356 GAGCAC G[AGT]GC[ACT]^C BsrDI 593 GCAATG GCAATG(2/0) BstYI 312 AGATCT [GA]^GATC[CT] Cac8I 457 GCAGGC GC[ACGT]^[ACGT]GC CelII 346 GCTAAGC GC^T[ACGT]AGC EaeI 697 TGGCCG [CT]^GGCC[GA] Eam1105I 622 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 340 GAAGAG CTCTTC(1/4) EcoNI 168 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 367 CCTTGG C^C[AT][AT]GG FauI 606 GCGGG CCCGC(4/6) FbaI 647 TGATCA T^GATCA HincII 198 GTCAAC GT[CT]^[GA]AC HindII 198 GTCAAC GT[CT]^[GA]AC HindIII 115 AAGCTT A^AGCTT HinfI 31 GATTC G^A[ACGT]TC Ksp632I 340 GAAGAG CTCTTC(1/4) MaeI 565 CTAG C^TAG MflI 312 AGATCT [GA]^GATC[CT] MseI 290 TTAA T^TAA MwoI 423 GCAACAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PaeR7I 263 CTCGAG C^TCGAG PflMI 362 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SmlI 263 CTCGAG C^T[CT][GA]AG StuI 459 AGGCCT AGG^CCT StyI 367 CCTTGG C^C[AT][AT]GG TaqI 264 TCGA T^CGA TfiI 31 GATTC G^A[AT]TC Tru9I 290 TTAA T^TAA Tsp45I 124 GTGAC ^GT[GC]AC TthHB8I 264 TCGA T^CGA Van91I 362 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 263 CTCGAG C^TCGAG XhoII 312 AGATCT [GA]^GATC[CT] XmnI 583 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbvI 429 GCAGC GCAGC(8/12) 666 GCTGC BsaJI 13 CCCTGG C^C[ACGT][ACGT]GG 367 CCTTGG BsmAI 270 GAGAC GTCTC(1/5) 391 GAGAC BssKI 14 CCTGG ^CC[ACGT]GG 602 CCAGG BstNI 14 CCTGG CC^[AT]GG 602 CCAGG BstXI 395 CCAGAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 560 CCAACCTAGTGG EcoRII 14 CCTGG ^CC[AT]GG 602 CCAGG Fnu4HI 429 GCAGC GC^[ACGT]GC 666 GCTGC HaeIII 460 GGCC GG^CC 698 GGCC HapII 7 CCGG C^CGG 420 CCGG HpaII 7 CCGG C^CGG 420 CCGG HphI 100 GGTGA GGTGA(8/7) 654 GGTGA ItaI 429 GCAGC GC^[ACGT]GC 666 GCTGC MaeIII 124 GTGAC ^GT[ACGT]AC 259 GTAAC MboII 233 GAAGA GAAGA(8/7) 340 GAAGA MspI 7 CCGG C^CGG 420 CCGG MvaI 14 CCTGG CC^[AT]GG 602 CCAGG NlaIII 615 CATG CATG^ 651 CATG ScrFI 14 CCTGG CC^[ACGT]GG 602 CCAGG TseI 429 GCAGC G^C[AT]GC 666 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AluI AvaII BanI BseRI BsiYI BslI BstF5I Cfr13I DdeI DpnII FokI MboI MnlI NdeII NlaIV Sau3AI Sau96I SfaNI Tsp509I