################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeIII HapII HindIII HpaI HpaII KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I Tsp509I Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AhdI 76 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 308 AGCT AG^CT Alw44I 88 GTGCAC G^TGCAC ApaLI 88 GTGCAC G^TGCAC Asp700I 304 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 76 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 88 GTGCAC G[AT]GC[AT]^C BamHI 3 GGATCC G^GATCC BanI 269 GGCGCC G^G[CT][GA]CC BanII 172 GAGCCC G[GA]GC[CT]^C BbeI 269 GGCGCC GGCGC^C BfaI 142 CTAG C^TAG BseRI 381 CTCCTC GAGGAG(10/8) BsgI 622 GTGCAG GTGCAG(16/14) BsiHKAI 88 GTGCAC G[AT]GC[AT]^C BsmBI 67 GAGACG CGTCTC(1/5) BsrDI 501 CATTGC GCAATG(2/0) BstAPI 81 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraII 454 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 76 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 304 GAAGAG CTCTTC(1/4) EcoO109I 454 AGGACCC [GA]G^G[ACGT]CC[CT] FauI 552 GCGGG CCCGC(4/6) HaeII 269 GGCGCC [GA]GCGC^[CT] HgaI 59 GACGC GACGC(5/10) HincII 208 GTTGAC GT[CT]^[GA]AC HindII 208 GTTGAC GT[CT]^[GA]AC HphI 326 TCACC GGTGA(8/7) KasI 269 GGCGCC G^GCGCC Ksp632I 304 GAAGAG CTCTTC(1/4) MaeI 142 CTAG C^TAG MaeII 147 ACGT A^CGT NarI 269 GGCGCC GG^CGCC NspI 509 ACATGC [GA]CATG^[CT] PpuMI 454 AGGACCC [GA]G^G[AT]CC[CT] SapI 304 GAAGAGC GCTCTTC(1/4) ScaI 569 AGTACT AGT^ACT SfoI 269 GGCGCC GGC^GCC SspI 436 AATATT AAT^ATT TfiI 364 GAATC G^A[AT]TC XbaI 141 TCTAGA T^CTAGA XcmI 347 CCACCATACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 304 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AciI 537 GCGG CCGC(-3/-1) 552 GCGG AcyI 59 GACGCC G[GA]^CG[CT]C 269 GGCGCC AfaI 570 GTAC GT^AC 627 GTAC AvaII 33 GGTCC G^G[AT]CC 455 GGACC BbvI 285 GCAGC GCAGC(8/12) 591 GCTGC BsaHI 59 GACGCC G[GA]^CG[CT]C 269 GGCGCC BsmFI 57 GGGAC GGGAC(10/14) 478 GGGAC BspMI 236 ACCTGC ACCTGC(4/8) 30 GCAGGT BsrI 45 CCAGT ACTGG(1/-1) 213 CCAGT BssKI 63 CCTGG ^CC[ACGT]GG 561 CCAGG BssSI 37 CACGAG CACGAG(-5/-1) 616 CTCGTG BstF5I 411 GGATG GGATG(2/0) 587 GGATG BstNI 63 CCTGG CC^[AT]GG 561 CCAGG BstXI 350 CCATACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 506 CCAACATGCTGG Cac8I 115 GCAAGC GC[ACGT]^[ACGT]GC 552 GCGGGC CfoI 247 GCGC GCG^C 270 GCGC Cfr13I 33 GGTCC G^G[ACGT]CC 455 GGACC EcoRII 63 CCTGG ^CC[AT]GG 561 CCAGG FokI 411 GGATG GGATG(9/13) 587 GGATG HhaI 247 GCGC GCG^C 270 GCGC Hin1I 59 GACGCC G[GA]^CG[CT]C 269 GGCGCC HinP1I 247 GCGC G^CGC 270 GCGC MseI 217 TTAA T^TAA 377 TTAA MslI 351 CATACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 528 CACAGTGATG MvaI 63 CCTGG CC^[AT]GG 561 CCAGG PleI 337 GACTC GAGTC(4/5) 480 GACTC PstI 238 CTGCAG CTGCA^G 370 CTGCAG RsaI 570 GTAC GT^AC 627 GTAC Sau96I 33 GGTCC G^G[ACGT]CC 455 GGACC ScrFI 63 CCTGG CC^[ACGT]GG 561 CCAGG SfcI 238 CTGCAG C^T[GA][CT]AG 370 CTGCAG TaqI 318 TCGA T^CGA 545 TCGA Tru9I 217 TTAA T^TAA 377 TTAA TseI 285 GCAGC G^C[AT]GC 591 GCTGC TspRI 368 CACTG CA[GC]TG[ACGT][ACGT]^ 530 CAGTG TthHB8I 318 TCGA T^CGA 545 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AlwI AlwNI BmyI BpmI BsiYI BslI BsmAI Bsp1286I BstYI DdeI DpnII Fnu4HI HinfI ItaI MboI MboII MflI MnlI MwoI NdeII NlaIII NlaIV Sau3AI SfaNI XhoII