(Circular) (Six-base) MAPSORT of: pFN21AE6247.seq Check: 2651 from: 1 to: 5341 pFN21AE6247 5341 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2250 2250 Size: 5341 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3151 278 Size: 53 83 186 936 1615 2468 Fragments arranged by size: 2468 1615 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2684 1937 Size: 747 4594 Acc65I G'GTAC_C Cuts at: 2662 2662 Size: 5341 AclI AA'CG_TT Cuts at: 1563 3468 3841 1563 Size: 1905 373 3063 Fragments arranged by size: 3063 1905 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3404 4788 5056 1632 Size: 1772 1384 268 1917 Fragments arranged by size: 1917 1772 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5144 AflIII A'CryG_T Cuts at: 1203 4240 5217 1203 Size: 3037 977 1327 Fragments arranged by size: 3037 1327 977 AgeI A'CCGG_T Cuts at: 4172 4172 Size: 5341 AhdI GACnn_n'nnGTC Cuts at: 2086 4070 2086 Size: 1984 3357 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5341 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 5309 AlwNI CAG_nnn'CTG Cuts at: 2242 2470 2635 3084 4656 2242 Size: 228 165 449 1572 2927 Fragments arranged by size: 2927 1572 449 228 165 ApaLI G'TGCA_C Cuts at: 2093 3398 4554 2093 Size: 1305 1156 2880 Fragments arranged by size: 2880 1305 1156 ApoI r'AATT_y Cuts at: 1493 2650 2876 3028 5137 1493 Size: 1157 226 152 2109 1697 Fragments arranged by size: 2109 1697 1157 226 152 AseI AT'TA_AT Cuts at: 160 1032 3895 160 Size: 872 2863 1606 Fragments arranged by size: 2863 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 AvaI C'yCGr_G Cuts at: 1870 1943 2666 1870 Size: 73 723 4545 Fragments arranged by size: 4545 723 73 BamHI G'GATC_C Cuts at: 2008 2671 3038 2008 Size: 663 367 4311 Fragments arranged by size: 4311 663 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2274 2662 4117 618 Size: 325 217 627 487 388 1455 1842 Fragments arranged by size: 1842 1455 627 487 388 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2660 1391 Size: 790 479 4072 Fragments arranged by size: 4072 790 479 BbeI G_GCGC'C Cuts at: 2278 2278 Size: 5341 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3532 3566 1903 Size: 34 1595 34 3678 Fragments arranged by size: 3678 1595 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3233 4443 3233 Size: 1210 4131 BclI T'GATC_A Cuts at: 1548 1548 Size: 5341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3952 136 Size: 107 122 71 631 605 2280 1525 Fragments arranged by size: 2280 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5336 5336 Size: 5341 BlpI GC'TnA_GC Cuts at: 2758 2758 Size: 5341 Bme1580I G_kGCm'C Cuts at: 1790 2097 2439 3402 4558 1790 Size: 307 342 963 1156 2573 Fragments arranged by size: 2573 1156 963 342 307 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5341 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3072 4030 442 Size: 416 53 83 399 24 1655 958 1753 Fragments arranged by size: 1753 1655 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5341 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2177 2378 4001 1239 Size: 851 87 201 1623 2579 Fragments arranged by size: 2579 1623 851 201 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3466 4331 4629 3466 Size: 865 298 4178 Fragments arranged by size: 4178 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4004 882 Size: 617 2505 2219 Fragments arranged by size: 2505 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3132 493 Size: 711 1928 2702 Fragments arranged by size: 2702 1928 711 BsaBI GATnn'nnATC Cuts at: 5335 5335 Size: 5341 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2065 2275 3148 3530 Size: 53 83 186 936 532 210 873 382 Cuts at: 3530 4165 275 Size: 635 1451 Fragments arranged by size: 1451 936 873 635 532 382 210 186 83 53 BsaWI w'CCGG_w Cuts at: 3774 4172 4446 4593 5004 3774 Size: 398 274 147 411 4111 Fragments arranged by size: 4111 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2150 2180 1178 Size: 30 165 30 747 30 4339 Fragments arranged by size: 4339 747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2377 1186 Size: 1191 4150 BseYI C'CCAG_C Cuts at: 2518 4544 5040 2518 Size: 2026 496 2819 Fragments arranged by size: 2819 2026 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2648 1163 Size: 573 912 3856 Fragments arranged by size: 3856 912 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2791 3552 4580 1540 Size: 275 186 790 761 1028 2301 Fragments arranged by size: 2301 1028 790 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2097 3402 3487 4558 2097 Size: 1305 85 1071 2880 Fragments arranged by size: 2880 1305 1071 85 BsmI GAATG_Cn' Cuts at: 2852 2945 2852 Size: 93 5248 BsmBI CGTCTCn'nnnn_ Cuts at: 2066 2066 Size: 5341 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2181 2439 2660 3402 3487 4558 Size: 399 307 84 258 221 742 85 1071 Cuts at: 4558 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 307 258 221 85 84 BspEI T'CCGG_A Cuts at: 5004 5004 Size: 5341 BspHI T'CATG_A Cuts at: 3230 4960 3230 Size: 1730 3611 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2026 2250 2696 844 Size: 1182 224 446 3489 Fragments arranged by size: 3489 1182 446 224 BsrBI CCG'CTC Cuts at: 1394 1823 3228 1394 Size: 429 1405 3507 Fragments arranged by size: 3507 1405 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2505 3836 4010 65 Size: 1183 1257 1331 174 1396 Fragments arranged by size: 1396 1331 1257 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3985 4172 1700 Size: 110 2175 187 2869 Fragments arranged by size: 2869 2175 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5341 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5341 BssSI C'ACGA_G Cuts at: 2042 2621 3401 4413 2042 Size: 579 780 1012 2970 Fragments arranged by size: 2970 1012 780 579 BstAPI GCAn_nnn'nTGC Cuts at: 794 2092 794 Size: 1298 4043 BstBI TT'CG_AA Cuts at: 4145 4145 Size: 5341 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5341 BstXI CCAn_nnnn'nTGG Cuts at: 2362 2518 2362 Size: 156 5185 BstYI r'GATC_y Cuts at: 1972 2008 2046 2496 2671 3038 3423 3440 4892 Size: 36 38 450 175 367 385 17 1452 Cuts at: 4892 4978 4990 5336 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 450 385 367 346 175 86 38 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3850 Fragments arranged by size: 3850 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4602 Fragments arranged by size: 4602 683 56 BtsI GCAGTG_nn' Cuts at: 2372 2857 3651 3671 2372 Size: 485 794 20 4042 Fragments arranged by size: 4042 794 485 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5341 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5306 DraI TTT'AAA Cuts at: 2645 2645 Size: 5341 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4348 809 Size: 507 3032 1802 Fragments arranged by size: 3032 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2788 3677 5306 Size: 54 1425 50 275 18 958 889 1629 Cuts at: 5306 8 Size: 43 Fragments arranged by size: 1629 1425 958 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2788 1537 Size: 275 976 4090 Fragments arranged by size: 4090 976 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2304 3271 1353 Size: 951 967 3423 Fragments arranged by size: 3423 967 951 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3928 4302 4448 387 Size: 1064 138 2339 374 146 1280 Fragments arranged by size: 2339 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2177 2378 3404 4001 4788 5056 Size: 393 458 87 201 1026 597 787 268 Cuts at: 5056 1239 Size: 1524 Fragments arranged by size: 1524 1026 787 597 458 393 268 201 87 EcoO109I rG'GnC_Cy Cuts at: 2460 2460 Size: 5341 EcoRI G'AATT_C Cuts at: 2650 3028 2650 Size: 378 4963 FspI TGC'GCA Cuts at: 3847 3847 Size: 5341 HaeII r_GCGC'y Cuts at: 1678 2278 4488 1678 Size: 600 2210 2531 Fragments arranged by size: 2531 2210 600 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2072 2104 2150 Size: 32 135 32 464 32 4 32 46 Cuts at: 2150 2182 2303 2335 3982 4014 4056 4088 1373 Size: 32 121 32 1647 32 42 32 2626 Fragments arranged by size: 2626 1647 464 135 121 46 42 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 2215 2685 2931 669 Size: 988 281 277 470 246 3079 Fragments arranged by size: 3079 988 470 281 277 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5341 HpaI GTT'AAC Cuts at: 2931 2931 Size: 5341 KasI G'GCGC_C Cuts at: 2274 2274 Size: 5341 KpnI G_GTAC'C Cuts at: 2666 2666 Size: 5341 MfeI C'AATT_G Cuts at: 2940 2940 Size: 5341 MluI A'CGCG_T Cuts at: 5217 5217 Size: 5341 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2553 4455 4639 1305 Size: 1248 1902 184 2007 Fragments arranged by size: 2007 1902 1248 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3519 Fragments arranged by size: 3519 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 2537 3299 3658 3817 518 Size: 648 1194 177 762 359 159 2042 Fragments arranged by size: 2042 1194 762 648 359 177 159 MspA1I CmG'CkG Cuts at: 1606 2757 3434 4582 4827 1606 Size: 1151 677 1148 245 2120 Fragments arranged by size: 2120 1151 1148 677 245 NarI GG'CG_CC Cuts at: 2275 2275 Size: 5341 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3850 Fragments arranged by size: 3850 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5341 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5341 NotI GC'GGCC_GC Cuts at: 2788 2788 Size: 5341 NspI r_CATG'y Cuts at: 1132 1229 2518 2699 4244 1132 Size: 97 1289 181 1545 2229 Fragments arranged by size: 2229 1545 1289 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5341 PciI A'CATG_T Cuts at: 4240 4240 Size: 5341 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5341 PfoI T'CCnGG_A Cuts at: 2565 2565 Size: 5341 PmeI GTTT'AAAC Cuts at: 2645 2645 Size: 5341 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3410 3442 4923 4955 3410 Size: 32 1481 32 3796 Fragments arranged by size: 3796 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2460 2460 Size: 5341 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5341 PsiI TTA'TAA Cuts at: 2911 2911 Size: 5341 PstI C_TGCA'G Cuts at: 830 2247 2379 2693 830 Size: 1417 132 314 3478 Fragments arranged by size: 3478 1417 314 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5341 SalI G'TCGA_C Cuts at: 1936 2683 1936 Size: 747 4594 SapI GCTCTTCn'nnn_ Cuts at: 2304 2304 Size: 5341 SbfI CC_TGCA'GG Cuts at: 2693 2693 Size: 5341 ScaI AGT'ACT Cuts at: 2576 3589 5301 2576 Size: 1013 1712 2616 Fragments arranged by size: 2616 1712 1013 SfcI C'TryA_G Cuts at: 826 1045 2243 2375 2689 3824 4505 4696 826 Size: 219 1198 132 314 1135 681 191 1471 Fragments arranged by size: 1471 1198 1135 681 314 219 191 132 SfoI GGC'GCC Cuts at: 2276 2276 Size: 5341 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5341 SmaI CCC'GGG Cuts at: 1872 2668 1872 Size: 796 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3445 4346 4608 820 Size: 197 926 1502 901 262 1553 Fragments arranged by size: 1553 1502 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3132 493 Size: 2639 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5341 SphI G_CATG'C Cuts at: 1132 2699 1132 Size: 1567 3774 SspI AAT'ATT Cuts at: 5 52 2443 3265 5 Size: 47 2391 822 2081 Fragments arranged by size: 2391 2081 822 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5341 StyI C'CwwG_G Cuts at: 513 1065 2004 2780 513 Size: 552 939 776 3074 Fragments arranged by size: 3074 939 776 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3368 3385 3538 3723 4168 325 Size: 1255 1788 17 153 185 445 1498 Fragments arranged by size: 1788 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2574 3587 5299 Size: 274 80 33 51 1443 597 1013 1712 Cuts at: 5299 96 Size: 138 Fragments arranged by size: 1712 1443 1013 597 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5301 5333 5301 Size: 32 5309 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5341 XbaI T'CTAG_A Cuts at: 2146 2677 2146 Size: 531 4810 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2359 Size: 5341 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5341 XmaI C'CCGG_G Cuts at: 1870 2666 1870 Size: 796 4545 XmnI GAAnn'nnTTC Cuts at: 2313 3470 2313 Size: 1157 4184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3149 276 Size: 53 83 186 936 1615 2468 Fragments arranged by size: 2468 1615 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI