################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaII AviII AvrII BalI BbrPI BcgI BciVI BcnI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacII SalI SapI Sau96I SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AcsI 770 AAATTC [GA]^AATT[CT] AcyI 358 GGCGCC G[GA]^CG[CT]C AlwNI 459 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 770 AAATTC [GA]^AATT[CT] AseI 1018 ATTAAT AT^TAAT AsnI 1018 ATTAAT AT^TAAT Asp700I 914 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 183 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 98 CTCGAG C^[CT]CG[GA]G BamHI 1004 GGATCC G^GATCC BanI 358 GGCGCC G^G[CT][GA]CC BbeI 358 GGCGCC GGCGC^C BbvCI 985 CCTCAGC CCTCAGC(-5/-2) BclI 1029 TGATCA T^GATCA BfaI 857 CTAG C^TAG BglI 283 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 388 GCTCAGC GC^T[ACGT]AGC BpmI 156 CTGGAG CTGGAG(16/14) Bpu1102I 388 GCTCAGC GC^T[ACGT]AGC BsaHI 358 GGCGCC G[GA]^CG[CT]C BsaI 124 GAGACC GGTCTC(1/5) BsgI 14 CTGCAC GTGCAG(16/14) BsmBI 239 CGTCTC CGTCTC(1/5) BsmFI 31 GTCCC GGGAC(10/14) BsoBI 98 CTCGAG C^[CT]CG[GA]G BspHI 651 TCATGA T^CATGA BspMI 18 ACCTGC ACCTGC(4/8) BsrDI 1033 CATTGC GCAATG(2/0) BstAPI 292 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 533 CCAAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1004 GGATCC [GA]^GATC[CT] CelII 388 GCTCAGC GC^T[ACGT]AGC DraI 942 TTTAAA TTT^AAA FauI 105 GCGGG CCCGC(4/6) FbaI 1029 TGATCA T^GATCA HaeII 358 GGCGCC [GA]GCGC^[CT] HaeIII 607 GGCC GG^CC HapII 63 CCGG C^CGG Hin1I 358 GGCGCC G[GA]^CG[CT]C HpaII 63 CCGG C^CGG KasI 358 GGCGCC G^GCGCC MaeI 857 CTAG C^TAG MflI 1004 GGATCC [GA]^GATC[CT] MspI 63 CCGG C^CGG NarI 358 GGCGCC GG^CGCC PaeR7I 98 CTCGAG C^TCGAG PflFI 183 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 210 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1018 ATTAAT AT^TAAT RcaI 651 TCATGA T^CATGA ScaI 402 AGTACT AGT^ACT SfoI 358 GGCGCC GGC^GCC SmlI 98 CTCGAG C^T[CT][GA]AG Tth111I 183 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 991 CCAGAAGATAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 98 CTCGAG C^TCGAG XhoII 1004 GGATCC [GA]^GATC[CT] XmnI 914 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AspHI 715 GAGCTC G[AT]GC[AT]^C 1054 GAGCTC BbsI 161 GAAGAC GAAGAC(2/6) 217 GTCTTC BpuAI 161 GAAGAC GAAGAC(2/6) 217 GTCTTC BsiHKAI 715 GAGCTC G[AT]GC[AT]^C 1054 GAGCTC BsmAI 124 GAGAC GTCTC(1/5) 240 GTCTC HphI 335 TCACC GGTGA(8/7) 900 TCACC MspA1I 176 CAGCTG C[AC]G^C[GT]G 817 CAGCTG NlaIV 358 GGCGCC GG[ACGT]^[ACGT]CC 1004 GGATCC PleI 164 GACTC GAGTC(4/5) 204 GACTC PstI 20 CTGCAG CTGCA^G 512 CTGCAG PvuII 176 CAGCTG CAG^CTG 817 CAGCTG SacI 715 GAGCTC GAGCT^C 1054 GAGCTC SfcI 20 CTGCAG C^T[GA][CT]AG 512 CTGCAG TaqI 99 TCGA T^CGA 144 TCGA TfiI 914 GAATC G^A[AT]TC 1046 GAATC TthHB8I 99 TCGA T^CGA 144 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AluI AlwI BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI DdeI DpnII EcoRII Fnu4HI FokI HhaI HinP1I HinfI ItaI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI