(Circular) (Six-base) MAPSORT of: pFN21AE1558.seq Check: 4807 from: 1 to: 5785 pFN21AE1558 5785 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2032 2032 Size: 5785 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2789 3848 5232 5500 1632 Size: 1157 1059 1384 268 1917 Fragments arranged by size: 1917 1384 1157 1059 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 2899 1166 Size: 1733 4052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2469 3528 5100 2469 Size: 1059 1572 3154 Fragments arranged by size: 3154 1572 1059 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 2775 3094 3320 3472 5581 1493 Size: 1282 319 226 152 2109 1697 Fragments arranged by size: 2109 1697 1282 319 226 152 AseI AT'TA_AT Cuts at: 160 1032 3024 4339 160 Size: 872 1992 1315 1606 Fragments arranged by size: 1992 1606 1315 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 2103 3110 1870 Size: 73 160 1007 4545 Fragments arranged by size: 4545 1007 160 73 BamHI G'GATC_C Cuts at: 3009 3115 3482 3009 Size: 106 367 5312 Fragments arranged by size: 5312 367 106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 3106 4561 618 Size: 325 217 627 576 743 1455 1842 Fragments arranged by size: 1842 1455 743 627 576 325 217 BanII G_rGCy'C Cuts at: 1391 2435 2724 3063 3104 1391 Size: 1044 289 339 41 4072 Fragments arranged by size: 4072 1044 339 289 41 BbeI G_GCGC'C Cuts at: 2367 2367 Size: 5785 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2173 2215 928 Size: 971 274 42 4498 Fragments arranged by size: 4498 971 274 42 BbvCI CC'TCA_GC Cuts at: 2991 2991 Size: 5785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 3034 1548 Size: 1486 4299 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2294 4396 136 Size: 107 122 71 631 605 622 2102 1525 Fragments arranged by size: 2102 1525 631 622 605 122 107 71 BglII A'GATC_T Cuts at: 5780 5780 Size: 5785 BlpI GC'TnA_GC Cuts at: 2394 3202 2394 Size: 808 4977 Bme1580I G_kGCm'C Cuts at: 1790 3846 5002 1790 Size: 2056 1156 2573 Fragments arranged by size: 2573 2056 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3516 4474 442 Size: 416 53 83 399 24 2099 958 1753 Fragments arranged by size: 2099 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2656 2656 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2182 4445 1239 Size: 943 2263 2579 Fragments arranged by size: 2579 2263 943 Bpu10I CC'TnA_GC Cuts at: 2991 2991 Size: 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3910 4775 5073 3910 Size: 865 298 4622 Fragments arranged by size: 4622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2123 4448 882 Size: 617 624 2325 2219 Fragments arranged by size: 2325 2219 624 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 5779 5779 Size: 5785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2364 3592 3974 4609 Size: 53 83 186 936 831 1228 382 635 Cuts at: 4609 275 Size: 1451 Fragments arranged by size: 1451 1228 936 831 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 1178 Size: 30 165 30 672 30 4858 Fragments arranged by size: 4858 672 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2153 2645 1186 Size: 910 57 492 4326 Fragments arranged by size: 4326 910 492 57 BseYI C'CCAG_C Cuts at: 2681 4988 5484 2681 Size: 2307 496 2982 Fragments arranged by size: 2982 2307 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2004 1163 Size: 573 268 4944 Fragments arranged by size: 4944 573 268 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2724 3063 3846 3931 5002 2724 Size: 339 783 85 1071 3507 Fragments arranged by size: 3507 1071 783 339 85 BsmI GAATG_Cn' Cuts at: 3296 3389 3296 Size: 93 5692 BsmBI CGTCTCn'nnnn_ Cuts at: 2250 2250 Size: 5785 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2435 2724 3063 3104 3846 3931 5002 Size: 399 645 289 339 41 742 85 1071 Cuts at: 5002 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 645 399 339 289 85 41 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 2656 3674 5404 2656 Size: 1018 1730 3037 Fragments arranged by size: 3037 1730 1018 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2032 3140 844 Size: 1188 1108 3489 Fragments arranged by size: 3489 1188 1108 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3037 4280 4454 65 Size: 1183 1789 1243 174 1396 Fragments arranged by size: 1789 1396 1243 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 2303 794 Size: 1509 4276 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2545 2545 Size: 5785 BstYI r'GATC_y Cuts at: 1972 3009 3115 3482 3867 3884 5336 5422 5434 Size: 1037 106 367 385 17 1452 86 12 Cuts at: 5434 5780 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1037 385 367 346 106 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5046 Fragments arranged by size: 5046 683 56 BtsI GCAGTG_nn' Cuts at: 2237 2313 3301 4095 4115 2237 Size: 76 988 794 20 3907 Fragments arranged by size: 3907 988 794 76 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 2949 3089 2949 Size: 140 5645 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750 Size: 54 1425 50 275 18 1402 889 1629 Cuts at: 5750 8 Size: 43 Fragments arranged by size: 1629 1425 1402 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3715 1353 Size: 2362 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 4372 4746 4892 387 Size: 1064 138 504 2279 374 146 1280 Fragments arranged by size: 2279 1280 1064 504 374 146 138 EcoICRI GAG'CTC Cuts at: 2722 3061 2722 Size: 339 5446 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2182 2789 3848 4445 5232 5500 1239 Size: 393 550 607 1059 597 787 268 1524 Fragments arranged by size: 1524 1059 787 607 597 550 393 268 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2367 4932 1678 Size: 689 2565 2531 Fragments arranged by size: 2565 2531 689 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2097 2129 2591 2623 2875 Size: 32 135 32 525 32 462 32 252 Cuts at: 2875 2907 4426 4458 4500 4532 1373 Size: 32 1519 32 42 32 2626 Fragments arranged by size: 2626 1519 525 462 252 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3129 3375 669 Size: 988 281 1191 246 3079 Fragments arranged by size: 3079 1191 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KasI G'GCGC_C Cuts at: 2363 2363 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2447 2987 4899 5083 1305 Size: 1142 540 1912 184 2007 Fragments arranged by size: 2007 1912 1142 540 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3963 Fragments arranged by size: 3963 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2655 2661 2899 3743 4102 4261 518 Size: 648 1489 6 238 844 359 159 2042 Fragments arranged by size: 2042 1489 844 648 359 238 159 6 MspA1I CmG'CkG Cuts at: 1606 2183 2824 3201 3878 5026 5271 1606 Size: 577 641 377 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 641 577 377 245 NarI GG'CG_CC Cuts at: 2364 2364 Size: 5785 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2219 1613 Size: 606 5179 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PspXI vC'TCGA_Gb Cuts at: 2103 2103 Size: 5785 PstI C_TGCA'G Cuts at: 830 2029 2521 3137 830 Size: 1199 492 616 3478 Fragments arranged by size: 3478 1199 616 492 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2183 2824 2183 Size: 641 5144 SacI G_AGCT'C Cuts at: 2724 3063 2724 Size: 339 5446 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 2409 4033 5745 2409 Size: 1624 1712 2449 Fragments arranged by size: 2449 1712 1624 SfcI C'TryA_G Cuts at: 826 1045 2025 2517 3133 4268 4949 5140 826 Size: 219 980 492 616 1135 681 191 1471 Fragments arranged by size: 1471 1135 980 681 616 492 219 191 SfoI GGC'GCC Cuts at: 2365 2365 Size: 5785 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2103 3889 4790 5052 820 Size: 197 926 160 1786 901 262 1553 Fragments arranged by size: 1786 1553 926 901 262 197 160 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 2004 3224 513 Size: 552 939 1220 3074 Fragments arranged by size: 3074 1220 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3812 3829 3982 4167 4612 325 Size: 1255 2232 17 153 185 445 1498 Fragments arranged by size: 2232 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 2407 4031 Size: 274 80 33 51 1443 333 97 1624 Cuts at: 4031 5743 96 Size: 1712 138 Fragments arranged by size: 1712 1624 1443 333 274 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 2191 1316 Size: 875 4910 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3003 3003 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 2103 1943 Size: 160 5625 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 2923 3914 2923 Size: 991 4794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI