################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BssHII Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MseI MunI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SmaI SmlI SnaBI SpeI Sse8387I SspI SwaI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 508 GGTACC G^GTACC AlwNI 7 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 858 CCTGAGG CC^T[ACGT]AGG ApaI 199 GGGCCC GGGCC^C Asp718I 508 GGTACC G^GTACC AspI 1063 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 622 CCCGAG C^[CT]CG[GA]G BbeI 1036 GGCGCC GGCGC^C BbrPI 93 CACGTG CAC^GTG BbvCI 306 CCTCAGC CCTCAGC(-5/-2) BcgI 624 CGAGACACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 93 CACGTG [CT]AC^GT[GA] BsmBI 441 CGTCTC CGTCTC(1/5) BsoBI 622 CCCGAG C^[CT]CG[GA]G Bsp1407I 719 TGTACA T^GTACA BspLU11I 1196 ACATGT A^CATGT BspMI 630 ACCTGC ACCTGC(4/8) BsrBI 1303 GAGCGG CCGCTC(-3/-3) BsrDI 123 GCAATG GCAATG(2/0) BsrGI 719 TGTACA T^GTACA BssSI 991 CTCGTG CACGAG(-5/-1) BstEII 63 GGTGACC G^GT[ACGT]ACC BstPI 63 GGTGACC G^GT[ACGT]ACC Bsu36I 858 CCTGAGG CC^T[ACGT]AGG DraIII 796 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 858 CCTGAGG CC^T[ACGT]AGG EcoNI 1427 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 63 GGTGACC G^GT[ACGT]ACC FseI 890 GGCCGGCC GGCCGG^CC KasI 1036 GGCGCC G^GCGCC KpnI 508 GGTACC GGTAC^C NarI 1036 GGCGCC GG^CGCC NdeI 782 CATATG CA^TATG NheI 901 GCTAGC G^CTAGC PflFI 1063 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 6 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1100 GACTC GAGTC(4/5) PmaCI 93 CACGTG CAC^GTG PmlI 93 CACGTG CAC^GTG SexAI 956 ACCTGGT A^CC[AT]GGT SfoI 1036 GGCGCC GGC^GCC SgrAI 403 CGCCGGCG C[GA]^CCGG[CT]G SphI 652 GCATGC GCATG^C SspBI 719 TGTACA T^GTACA TfiI 332 GATTC G^A[AT]TC Tth111I 1063 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 6 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccII 423 CGCG CG^CG 1287 CGCG AflIII 94 ACGTGT A^C[GA][CT]GT 1196 ACATGT AspHI 184 GTGCTC G[AT]GC[AT]^C 988 GTGCTC BcnI 872 CCGGG CC^[GC]GG 1151 CCGGG BfaI 868 CTAG C^TAG 902 CTAG BsaI 151 GAGACC GGTCTC(1/5) 595 GAGACC BseRI 823 CTCCTC GAGGAG(10/8) 1428 CTCCTC BsiEI 657 CGGCCG CG[GA][CT]^CG 889 CGGCCG BsiHKAI 184 GTGCTC G[AT]GC[AT]^C 988 GTGCTC BsrFI 404 GCCGGC [GA]^CCGG[CT] 891 GCCGGC BstUI 423 CGCG CG^CG 1287 CGCG BstYI 530 GGATCT [GA]^GATC[CT] 1308 GGATCT Cfr10I 404 GCCGGC [GA]^CCGG[CT] 891 GCCGGC DsaI 59 CCATGG C^C[GA][CT]GG 1073 CCATGG EagI 657 CGGCCG C^GGCCG 889 CGGCCG EarI 1102 CTCTTC CTCTTC(1/4) 1228 CTCTTC EclXI 657 CGGCCG C^GGCCG 889 CGGCCG Eco52I 657 CGGCCG C^GGCCG 889 CGGCCG EcoT14I 59 CCATGG C^C[AT][AT]GG 1073 CCATGG HaeII 671 AGCGCC [GA]GCGC^[CT] 1036 GGCGCC HinfI 332 GATTC G^A[ACGT]TC 1100 GACTC Ksp632I 1102 CTCTTC CTCTTC(1/4) 1228 CTCTTC MaeI 868 CTAG C^TAG 902 CTAG MaeII 94 ACGT A^CGT 1386 ACGT MflI 530 GGATCT [GA]^GATC[CT] 1308 GGATCT MvnI 423 CGCG CG^CG 1287 CGCG NaeI 404 GCCGGC GCC^GGC 891 GCCGGC NciI 872 CCGGG CC^[GC]GG 1151 CCGGG NcoI 59 CCATGG C^CATGG 1073 CCATGG NgoMIV 404 GCCGGC G^CCGGC 891 GCCGGC PstI 1255 CTGCAG CTGCA^G 1474 CTGCAG SfcI 1255 CTGCAG C^T[GA][CT]AG 1474 CTGCAG StuI 342 AGGCCT AGG^CCT 568 AGGCCT StyI 59 CCATGG C^C[AT][AT]GG 1073 CCATGG TaqI 433 TCGA T^CGA 686 TCGA TthHB8I 433 TCGA T^CGA 686 TCGA XcmI 269 CCAGCCTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 781 CCATATGCCATGTGG XhoII 530 GGATCT [GA]^GATC[CT] 1308 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AvaII BalI BanI BanII BbsI BbvI BmyI BpmI BpuAI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI