(Circular) (Six-base) MAPSORT of: pFN21AE6446.seq Check: 3510 from: 1 to: 6220 pFN21AE6446 6220 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2644 2644 Size: 6220 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 2513 3541 2513 Size: 1028 5192 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2347 2833 4283 5667 5935 1632 Size: 715 486 1450 1384 268 1917 Fragments arranged by size: 1917 1450 1384 715 486 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 2099 3201 5119 6096 1203 Size: 896 1102 1918 977 1327 Fragments arranged by size: 1918 1327 1102 977 896 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 2066 2186 2588 1166 Size: 900 120 402 4798 Fragments arranged by size: 4798 900 402 120 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2402 2662 1184 Size: 1218 260 4742 Fragments arranged by size: 4742 1218 260 AlwNI CAG_nnn'CTG Cuts at: 2017 3963 5535 2017 Size: 1946 1572 2702 Fragments arranged by size: 2702 1946 1572 ApaI G_GGCC'C Cuts at: 2208 2208 Size: 6220 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 3529 3755 3907 6016 1493 Size: 2036 226 152 2109 1697 Fragments arranged by size: 2109 2036 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2627 3545 1870 Size: 73 684 918 4545 Fragments arranged by size: 4545 918 684 73 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2513 2782 3041 3541 4996 Size: 325 217 627 726 269 259 500 1455 Cuts at: 4996 618 Size: 1842 Fragments arranged by size: 1842 1455 726 627 500 325 269 259 217 BanII G_rGCy'C Cuts at: 1391 2208 2955 3100 3539 1391 Size: 817 747 145 439 4072 Fragments arranged by size: 4072 817 747 439 145 BbeI G_GCGC'C Cuts at: 3045 3045 Size: 6220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2518 2767 3369 928 Size: 971 619 249 602 3779 Fragments arranged by size: 3779 971 619 602 249 BbvCI CC'TCA_GC Cuts at: 2312 2312 Size: 6220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2618 2652 4411 4445 1903 Size: 34 50 34 597 34 1759 34 3678 Fragments arranged by size: 3678 1759 597 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 2208 4281 5437 1790 Size: 418 2073 1156 2573 Fragments arranged by size: 2573 2073 1156 418 BmgBI CAC'GTC Cuts at: 1318 3392 1318 Size: 2074 4146 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2741 3951 4909 Size: 416 53 83 399 24 1324 1210 958 Cuts at: 4909 442 Size: 1753 Fragments arranged by size: 1753 1324 1210 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2910 1056 Size: 1854 4366 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2513 2527 3326 3421 4880 1239 Size: 800 474 14 799 95 1459 2579 Fragments arranged by size: 2579 1459 800 799 474 95 14 Bpu10I CC'TnA_GC Cuts at: 2312 2312 Size: 6220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4345 5210 5508 4345 Size: 865 298 5057 Fragments arranged by size: 5057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2150 2594 4883 882 Size: 617 651 444 2289 2219 Fragments arranged by size: 2289 2219 651 617 444 BsaAI yAC'GTr Cuts at: 493 1204 2100 4011 493 Size: 711 896 1911 2702 Fragments arranged by size: 2702 1911 896 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2445 3042 3171 3402 Size: 53 83 186 936 912 597 129 231 Cuts at: 3402 4027 4409 5044 275 Size: 625 382 635 1451 Fragments arranged by size: 1451 936 912 635 625 597 382 231 186 129 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5995 Fragments arranged by size: 5995 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2819 3424 1186 Size: 1633 605 3982 Fragments arranged by size: 3982 1633 605 BseYI C'CCAG_C Cuts at: 2037 2257 2389 5423 5919 2037 Size: 220 132 3034 496 2338 Fragments arranged by size: 3034 2338 496 220 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2046 2233 2622 1163 Size: 573 310 187 389 4761 Fragments arranged by size: 4761 573 389 310 187 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2665 2897 3670 4431 5459 1540 Size: 275 186 664 232 773 761 1028 2301 Fragments arranged by size: 2301 1028 773 761 664 275 232 186 BsiHKAI G_wGCw'C Cuts at: 2193 2997 4281 4366 5437 2193 Size: 804 1284 85 1071 2976 Fragments arranged by size: 2976 1284 1071 804 85 BsmI GAATG_Cn' Cuts at: 3731 3824 3731 Size: 93 6127 BsmBI CGTCTCn'nnnn_ Cuts at: 2452 2452 Size: 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2208 2955 2997 3100 3539 4281 Size: 399 403 15 747 42 103 439 742 Cuts at: 4281 4366 5437 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 747 742 439 403 399 103 85 42 15 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2644 3575 844 Size: 1800 931 3489 Fragments arranged by size: 3489 1800 931 BsrBI CCG'CTC Cuts at: 1394 1823 3310 4107 1394 Size: 429 1487 797 3507 Fragments arranged by size: 3507 1487 797 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2135 4715 4889 65 Size: 1183 887 2580 174 1396 Fragments arranged by size: 2580 1396 1183 887 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2409 2896 4864 5051 1700 Size: 110 599 487 1968 187 2869 Fragments arranged by size: 2869 1968 599 487 187 110 BsrGI T'GTAC_A Cuts at: 96 2724 96 Size: 2628 3592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 2996 4280 5292 2996 Size: 1284 1012 3924 Fragments arranged by size: 3924 1284 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 2068 1184 Size: 884 5336 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2590 3100 2431 Size: 159 510 5551 Fragments arranged by size: 5551 510 159 BstYI r'GATC_y Cuts at: 1972 2535 3313 3550 3917 4302 4319 5771 5857 Size: 563 778 237 367 385 17 1452 86 Cuts at: 5857 5869 6215 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 778 563 385 367 346 237 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2864 2864 Size: 6220 BtgI C'CryG_G Cuts at: 513 1065 2064 3078 513 Size: 552 999 1014 3655 Fragments arranged by size: 3655 1014 999 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2545 2762 3736 4530 4550 2545 Size: 217 974 794 20 4215 Fragments arranged by size: 4215 974 794 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DraIII CAC_nnn'GTG Cuts at: 2806 2806 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2271 2289 Size: 54 1425 50 275 18 228 213 18 Cuts at: 2289 2421 2468 2580 2662 2894 3153 3464 3667 Size: 132 47 112 82 232 259 311 203 Cuts at: 3667 4556 6185 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 311 275 259 232 228 213 203 132 112 82 54 50 47 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2662 2894 3667 1537 Size: 275 850 232 773 4090 Fragments arranged by size: 4090 850 773 275 232 EarI CTCTTCn'nnn_ Cuts at: 1353 3113 3239 4150 1353 Size: 1760 126 911 3423 Fragments arranged by size: 3423 1760 911 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2396 3311 4807 5181 5327 387 Size: 1064 138 807 915 1496 374 146 1280 Fragments arranged by size: 1496 1280 1064 915 807 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2347 2513 2527 2833 3326 3421 Size: 393 407 308 166 14 306 493 95 Cuts at: 3421 4283 4880 5667 5935 1239 Size: 862 597 787 268 1524 Fragments arranged by size: 1524 862 787 597 493 407 393 308 306 268 166 95 14 EcoNI CCTnn'n_nnAGG Cuts at: 3436 3436 Size: 6220 EcoO109I rG'GnC_Cy Cuts at: 2014 2077 2204 2205 2868 2014 Size: 63 127 1 663 5366 Fragments arranged by size: 5366 663 127 63 1 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 FseI GG_CCGG'CC Cuts at: 2900 2900 Size: 6220 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2680 3045 5367 1678 Size: 1002 365 2322 2531 Fragments arranged by size: 2531 2322 1002 365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4861 4893 4935 4967 1373 Size: 32 135 32 3289 32 42 32 2626 Fragments arranged by size: 3289 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KasI G'GCGC_C Cuts at: 3041 3041 Size: 6220 KpnI G_GTAC'C Cuts at: 2517 3545 2517 Size: 1028 5192 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5334 5518 1305 Size: 4029 184 2007 Fragments arranged by size: 4029 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 2273 2291 2423 2582 Size: 54 1425 343 228 213 18 132 159 Cuts at: 2582 3466 10 Size: 884 2764 Fragments arranged by size: 2764 1425 884 343 228 213 159 132 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2066 2186 2222 2384 2588 4178 4537 Size: 648 900 120 36 162 204 1590 359 Cuts at: 4537 4696 518 Size: 159 2042 Fragments arranged by size: 2042 1590 900 648 359 204 162 159 120 36 MspA1I CmG'CkG Cuts at: 1606 2037 2473 3227 3398 3636 4313 5461 5706 Size: 431 436 754 171 238 677 1148 245 Cuts at: 5706 1606 Size: 2120 Fragments arranged by size: 2120 1148 754 677 436 431 245 238 171 NaeI GCC'GGC Cuts at: 2411 2898 2411 Size: 487 5733 NarI GG'CG_CC Cuts at: 3042 3042 Size: 6220 NcoI C'CATG_G Cuts at: 513 1065 2064 3078 513 Size: 552 999 1014 3655 Fragments arranged by size: 3655 1014 999 552 NdeI CA'TA_TG Cuts at: 387 2788 387 Size: 2401 3819 NgoMIV G'CCGG_C Cuts at: 2409 2896 2409 Size: 487 5733 NheI G'CTAG_C Cuts at: 1052 2906 1052 Size: 1854 4366 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2359 2661 3205 3578 5123 1132 Size: 97 1130 302 544 373 1545 2229 Fragments arranged by size: 2229 1545 1130 544 373 302 97 PasI CC'CwG_GG Cuts at: 1348 2074 2086 2452 3100 1348 Size: 726 12 366 648 4468 Fragments arranged by size: 4468 726 648 366 12 PciI A'CATG_T Cuts at: 3201 5119 3201 Size: 1918 4302 PflMI CCAn_nnn'nTGG Cuts at: 1108 2017 1108 Size: 909 5311 PfoI T'CCnGG_A Cuts at: 3117 3165 3437 3117 Size: 48 272 5900 Fragments arranged by size: 5900 272 48 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PmlI CAC'GTG Cuts at: 2100 2100 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2204 2204 Size: 6220 PstI C_TGCA'G Cuts at: 830 3264 3483 3572 830 Size: 2434 219 89 3478 Fragments arranged by size: 3478 2434 219 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2037 3227 3398 2037 Size: 1190 171 4859 Fragments arranged by size: 4859 1190 171 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SexAI A'CCwGG_T Cuts at: 2961 2961 Size: 6220 SfcI C'TryA_G Cuts at: 826 1045 3260 3479 3568 4703 5384 5575 826 Size: 219 2215 219 89 1135 681 191 1471 Fragments arranged by size: 2215 1471 1135 681 219 219 191 89 SfoI GGC'GCC Cuts at: 3043 3043 Size: 6220 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 2409 1700 Size: 709 5511 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4324 5225 5487 820 Size: 197 926 2381 901 262 1553 Fragments arranged by size: 2381 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 2661 3578 1132 Size: 1529 917 3774 Fragments arranged by size: 3774 1529 917 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 2349 2575 1517 Size: 832 226 5162 Fragments arranged by size: 5162 832 226 StyI C'CwwG_G Cuts at: 513 1065 2064 3078 3659 513 Size: 552 999 1014 581 3074 Fragments arranged by size: 3074 1014 999 581 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2035 2723 2980 3230 4247 4264 4417 Size: 1255 455 688 257 250 1017 17 153 Cuts at: 4417 4602 5047 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1017 688 455 445 257 250 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2724 4466 6178 Size: 274 80 33 51 1443 747 1742 1712 Cuts at: 6178 96 Size: 138 Fragments arranged by size: 1742 1712 1443 747 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2760 2792 6180 6212 2760 Size: 32 3388 32 2768 Fragments arranged by size: 3388 2768 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3071 1316 Size: 1755 4465 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 2793 2281 Size: 512 5708 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 4349 4349 Size: 6220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FalI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI