(Linear) (Six-base) MAPSORT of: KIEE6446.seq Check: 1751 from: 1 to: 1515 KIEE6446.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 639 1515 Size: 639 876 Acc65I G'GTAC_C Cuts at: 0 508 1515 Size: 508 1007 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 342 828 1515 Size: 342 486 687 Fragments arranged by size: 687 486 342 AflIII A'CryG_T Cuts at: 0 94 1196 1515 Size: 94 1102 319 Fragments arranged by size: 1102 319 94 AleI CACnn'nnGTG Cuts at: 0 61 181 583 1515 Size: 61 120 402 932 Fragments arranged by size: 932 402 120 61 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 397 657 1515 Size: 397 260 858 Fragments arranged by size: 858 397 260 AlwNI CAG_nnn'CTG Cuts at: 0 12 1515 Size: 12 1503 ApaI G_GGCC'C Cuts at: 0 203 1515 Size: 203 1312 AvaI C'yCGr_G Cuts at: 0 622 1515 Size: 622 893 BanI G'GyrC_C Cuts at: 0 508 777 1036 1515 Size: 508 269 259 479 Fragments arranged by size: 508 479 269 259 BanII G_rGCy'C Cuts at: 0 203 950 1095 1515 Size: 203 747 145 420 Fragments arranged by size: 747 420 203 145 BbeI G_GCGC'C Cuts at: 0 1040 1515 Size: 1040 475 BbsI GAAGACnn'nnnn_ Cuts at: 0 513 762 1364 1515 Size: 513 249 602 151 Fragments arranged by size: 602 513 249 151 BbvCI CC'TCA_GC Cuts at: 0 307 1515 Size: 307 1208 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 613 647 1515 Size: 613 34 868 Fragments arranged by size: 868 613 34 Bme1580I G_kGCm'C Cuts at: 0 203 1515 Size: 203 1312 BmgBI CAC'GTC Cuts at: 0 1387 1515 Size: 1387 128 BmrI ACTGGGnnnn_n' Cuts at: 0 736 1515 Size: 736 779 BmtI G_CTAG'C Cuts at: 0 905 1515 Size: 905 610 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 508 522 1321 1416 1515 Size: 34 474 14 799 95 99 Fragments arranged by size: 799 474 99 95 34 14 Bpu10I CC'TnA_GC Cuts at: 0 307 1515 Size: 307 1208 BsaI GGTCTCn'nnnn_ Cuts at: 0 145 589 1515 Size: 145 444 926 Fragments arranged by size: 926 444 145 BsaAI yAC'GTr Cuts at: 0 95 1515 Size: 95 1420 BsaHI Gr'CG_yC Cuts at: 0 440 1037 1166 1397 1515 Size: 440 597 129 231 118 Fragments arranged by size: 597 440 231 129 118 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 814 1419 1515 Size: 814 605 96 Fragments arranged by size: 814 605 96 BseYI C'CCAG_C Cuts at: 0 32 252 384 1515 Size: 32 220 132 1131 Fragments arranged by size: 1131 220 132 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 228 617 1515 Size: 41 187 389 898 Fragments arranged by size: 898 389 187 41 BsiEI CG_ry'CG Cuts at: 0 660 892 1515 Size: 660 232 623 Fragments arranged by size: 660 623 232 BsiHKAI G_wGCw'C Cuts at: 0 188 992 1515 Size: 188 804 523 Fragments arranged by size: 804 523 188 BsmBI CGTCTCn'nnnn_ Cuts at: 0 447 1515 Size: 447 1068 Bsp1286I G_dGCh'C Cuts at: 0 188 203 950 992 1095 1515 Size: 188 15 747 42 103 420 Fragments arranged by size: 747 420 188 103 42 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 639 1515 Size: 639 876 BsrBI CCG'CTC Cuts at: 0 1305 1515 Size: 1305 210 BsrDI GCAATG_nn' Cuts at: 0 130 1515 Size: 130 1385 BsrFI r'CCGG_y Cuts at: 0 404 891 1515 Size: 404 487 624 Fragments arranged by size: 624 487 404 BsrGI T'GTAC_A Cuts at: 0 719 1515 Size: 719 796 BssSI C'ACGA_G Cuts at: 0 991 1515 Size: 991 524 BstEII G'GTnAC_C Cuts at: 0 63 1515 Size: 63 1452 BstXI CCAn_nnnn'nTGG Cuts at: 0 426 585 1095 1515 Size: 426 159 510 420 Fragments arranged by size: 510 426 420 159 BstYI r'GATC_y Cuts at: 0 530 1308 1515 Size: 530 778 207 Fragments arranged by size: 778 530 207 Bsu36I CC'TnA_GG Cuts at: 0 859 1515 Size: 859 656 BtgI C'CryG_G Cuts at: 0 59 1073 1515 Size: 59 1014 442 Fragments arranged by size: 1014 442 59 BtsI GCAGTG_nn' Cuts at: 0 540 757 1515 Size: 540 217 758 Fragments arranged by size: 758 540 217 DraIII CAC_nnn'GTG Cuts at: 0 801 1515 Size: 801 714 EaeI y'GGCC_r Cuts at: 0 53 266 284 416 463 575 657 889 Size: 53 213 18 132 47 112 82 232 Cuts at: 889 1148 1459 1515 Size: 259 311 56 Fragments arranged by size: 311 259 232 213 132 112 82 56 53 47 18 EagI C'GGCC_G Cuts at: 0 657 889 1515 Size: 657 232 626 Fragments arranged by size: 657 626 232 EarI CTCTTCn'nnn_ Cuts at: 0 1108 1234 1515 Size: 1108 126 281 Fragments arranged by size: 1108 281 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 391 1306 1515 Size: 391 915 209 Fragments arranged by size: 915 391 209 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 342 508 522 828 1321 1416 1515 Size: 34 308 166 14 306 493 95 99 Fragments arranged by size: 493 308 306 166 99 95 34 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 1431 1515 Size: 1431 84 EcoO109I rG'GnC_Cy Cuts at: 0 9 72 199 200 863 1515 Size: 9 63 127 1 663 652 Fragments arranged by size: 663 652 127 63 9 1 FseI GG_CCGG'CC Cuts at: 0 895 1515 Size: 895 620 HaeII r_GCGC'y Cuts at: 0 675 1040 1515 Size: 675 365 475 Fragments arranged by size: 675 475 365 KasI G'GCGC_C Cuts at: 0 1036 1515 Size: 1036 479 KpnI G_GTAC'C Cuts at: 0 512 1515 Size: 512 1003 MscI TGG'CCA Cuts at: 0 55 268 286 418 577 1461 1515 Size: 55 213 18 132 159 884 54 Fragments arranged by size: 884 213 159 132 55 54 18 MslI CAynn'nnrTG Cuts at: 0 61 181 217 379 583 1515 Size: 61 120 36 162 204 932 Fragments arranged by size: 932 204 162 120 61 36 MspA1I CmG'CkG Cuts at: 0 32 468 1222 1393 1515 Size: 32 436 754 171 122 Fragments arranged by size: 754 436 171 122 32 NaeI GCC'GGC Cuts at: 0 406 893 1515 Size: 406 487 622 Fragments arranged by size: 622 487 406 NarI GG'CG_CC Cuts at: 0 1037 1515 Size: 1037 478 NcoI C'CATG_G Cuts at: 0 59 1073 1515 Size: 59 1014 442 Fragments arranged by size: 1014 442 59 NdeI CA'TA_TG Cuts at: 0 783 1515 Size: 783 732 NgoMIV G'CCGG_C Cuts at: 0 404 891 1515 Size: 404 487 624 Fragments arranged by size: 624 487 404 NheI G'CTAG_C Cuts at: 0 901 1515 Size: 901 614 NspI r_CATG'y Cuts at: 0 354 656 1200 1515 Size: 354 302 544 315 Fragments arranged by size: 544 354 315 302 PasI CC'CwG_GG Cuts at: 0 69 81 447 1095 1515 Size: 69 12 366 648 420 Fragments arranged by size: 648 420 366 69 12 PciI A'CATG_T Cuts at: 0 1196 1515 Size: 1196 319 PflMI CCAn_nnn'nTGG Cuts at: 0 12 1515 Size: 12 1503 PfoI T'CCnGG_A Cuts at: 0 1112 1160 1432 1515 Size: 1112 48 272 83 Fragments arranged by size: 1112 272 83 48 PmlI CAC'GTG Cuts at: 0 95 1515 Size: 95 1420 PspOMI G'GGCC_C Cuts at: 0 199 1515 Size: 199 1316 PstI C_TGCA'G Cuts at: 0 1259 1478 1515 Size: 1259 219 37 Fragments arranged by size: 1259 219 37 PvuII CAG'CTG Cuts at: 0 32 1222 1393 1515 Size: 32 1190 171 122 Fragments arranged by size: 1190 171 122 32 SexAI A'CCwGG_T Cuts at: 0 956 1515 Size: 956 559 SfcI C'TryA_G Cuts at: 0 1255 1474 1515 Size: 1255 219 41 Fragments arranged by size: 1255 219 41 SfoI GGC'GCC Cuts at: 0 1038 1515 Size: 1038 477 SgrAI Cr'CCGG_yG Cuts at: 0 404 1515 Size: 404 1111 SphI G_CATG'C Cuts at: 0 656 1515 Size: 656 859 StuI AGG'CCT Cuts at: 0 344 570 1515 Size: 344 226 945 Fragments arranged by size: 945 344 226 StyI C'CwwG_G Cuts at: 0 59 1073 1515 Size: 59 1014 442 Fragments arranged by size: 1014 442 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 30 718 975 1225 1515 Size: 30 688 257 250 290 Fragments arranged by size: 688 290 257 250 30 TatI w'GTAC_w Cuts at: 0 719 1515 Size: 719 796 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 755 787 1515 Size: 755 32 728 Fragments arranged by size: 755 728 32 Tth111I GACn'n_nGTC Cuts at: 0 1066 1515 Size: 1066 449 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 276 788 1515 Size: 276 512 727 Fragments arranged by size: 727 512 276 Enzymes that do cut: AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FseI HaeII KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI PfoI PmlI PspOMI PstI PvuII SexAI SfcI SfoI SgrAI SphI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglI BglII BlpI BplI BpuEI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI MmeI NotI NruI NsiI PacI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SmaI SmlI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI