################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MboII MfeI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AlwI 66 GGATC GGATC(4/5) AspHI 227 GAGCTC G[AT]GC[AT]^C AvaII 6 GGACC G^G[AT]CC BanII 227 GAGCTC G[GA]GC[CT]^C BcgI 137 GCAAAATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 227 GAGCTC G[AGT]GC[ACT]^C BsaBI 48 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 87 CCTGGG C^C[ACGT][ACGT]GG BsiHKAI 227 GAGCTC G[AT]GC[AT]^C BsiYI 57 CCCAGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 57 CCCAGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 5 GGGAC GGGAC(10/14) Bsp1286I 227 GAGCTC G[AGT]GC[ACT]^C BsrDI 234 CATTGC GCAATG(2/0) BstBI 253 TTCGAA TT^CGAA BstYI 66 GGATCT [GA]^GATC[CT] Cfr13I 6 GGACC G^G[ACGT]CC DraII 5 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 5 GGGACCC [GA]G^G[ACGT]CC[CT] HphI 187 GGTGA GGTGA(8/7) MamI 48 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 66 GGATCT [GA]^GATC[CT] NspV 253 TTCGAA TT^CGAA PpuMI 5 GGGACCC [GA]G^G[AT]CC[CT] PstI 158 CTGCAG CTGCA^G SacI 227 GAGCTC GAGCT^C Sau96I 6 GGACC G^G[ACGT]CC SfcI 158 CTGCAG C^T[GA][CT]AG SfuI 253 TTCGAA TT^CGAA SmlI 199 CTTGAG C^T[CT][GA]AG StuI 84 AGGCCT AGG^CCT TaqI 254 TCGA T^CGA TthHB8I 254 TCGA T^CGA XcmI 53 CCATCCCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 66 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 24 GTAC GT^AC 204 GTAC AluI 77 AGCT AG^CT 228 AGCT BbvI 128 GCAGC GCAGC(8/12) 160 GCAGC BpmI 42 CTGGAG CTGGAG(16/14) 222 CTGGAG BssKI 87 CCTGG ^CC[ACGT]GG 168 CCAGG BstNI 87 CCTGG CC^[AT]GG 168 CCAGG Cac8I 150 GCAAGC GC[ACGT]^[ACGT]GC 238 GCCAGC DpnII 67 GATC ^GATC 144 GATC EcoRII 87 CCTGG ^CC[AT]GG 168 CCAGG Fnu4HI 128 GCAGC GC^[ACGT]GC 160 GCAGC HaeIII 85 GGCC GG^CC 193 GGCC ItaI 128 GCAGC GC^[ACGT]GC 160 GCAGC MboI 67 GATC ^GATC 144 GATC MvaI 87 CCTGG CC^[AT]GG 168 CCAGG NdeII 67 GATC ^GATC 144 GATC NlaIV 5 GGGACC GG[ACGT]^[ACGT]CC 6 GGACCC RsaI 24 GTAC GT^AC 204 GTAC Sau3AI 67 GATC ^GATC 144 GATC ScrFI 87 CCTGG CC^[ACGT]GG 168 CCAGG SfaNI 48 GATGC GCATC(5/9) 242 GCATC TseI 128 GCAGC G^C[AT]GC 160 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### BsrI BstF5I FokI MnlI MwoI