(Circular) (Six-base) MAPSORT of: pFN21AE4370.seq Check: 6001 from: 1 to: 5866 pFN21AE4370 5866 bp With 182 enzymes: SgfI * March 28, 2012 14:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2210 3209 1937 Size: 273 999 4594 Fragments arranged by size: 4594 999 273 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3929 5313 5581 1632 Size: 2297 1384 268 1917 Fragments arranged by size: 2297 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2053 1184 Size: 869 4997 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2374 2406 2374 Size: 32 5834 AlwNI CAG_nnn'CTG Cuts at: 2366 3609 5181 2366 Size: 1243 1572 3051 Fragments arranged by size: 3051 1572 1243 ApaI G_GGCC'C Cuts at: 2677 2677 Size: 5866 ApaLI G'TGCA_C Cuts at: 3923 5079 3923 Size: 1156 4710 ApoI r'AATT_y Cuts at: 1493 2917 3175 3401 3553 5662 1493 Size: 1424 258 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 258 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 3191 1870 Size: 73 1248 4545 Fragments arranged by size: 4545 1248 73 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2816 3187 4642 618 Size: 325 217 627 1029 371 1455 1842 Fragments arranged by size: 1842 1455 1029 627 371 325 217 BanII G_rGCy'C Cuts at: 1391 2096 2677 3005 3133 3185 1391 Size: 705 581 328 128 52 4072 Fragments arranged by size: 4072 705 581 328 128 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2889 928 Size: 971 990 3905 Fragments arranged by size: 3905 990 971 BbvCI CC'TCA_GC Cuts at: 2367 2871 2367 Size: 504 5362 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2367 2401 2413 2447 4057 4091 1903 Size: 34 430 34 12 34 1610 34 3678 Fragments arranged by size: 3678 1610 430 34 34 34 34 12 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 2496 2931 1548 Size: 948 435 4483 Fragments arranged by size: 4483 948 435 BfrBI ATG'CAT Cuts at: 2568 2568 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2130 4477 136 Size: 107 122 71 631 605 458 2347 1525 Fragments arranged by size: 2347 1525 631 605 458 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 2677 3927 5083 1790 Size: 887 1250 1156 2573 Fragments arranged by size: 2573 1250 1156 887 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2768 2811 2861 4526 1239 Size: 1529 43 50 1665 2579 Fragments arranged by size: 2579 1665 1529 50 43 Bpu10I CC'TnA_GC Cuts at: 2095 2367 2871 2095 Size: 272 504 5090 Fragments arranged by size: 5090 504 272 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3991 4856 5154 3991 Size: 865 298 4703 Fragments arranged by size: 4703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4529 882 Size: 617 3030 2219 Fragments arranged by size: 3030 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 5860 5860 Size: 5866 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4055 4690 275 Size: 53 83 186 936 2140 382 635 1451 Fragments arranged by size: 2140 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2962 4299 4697 4971 5118 5529 2962 Size: 1337 398 274 147 411 3299 Fragments arranged by size: 3299 1337 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2374 2404 1178 Size: 30 165 30 971 30 4640 Fragments arranged by size: 4640 971 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2682 1186 Size: 1496 4370 BseYI C'CCAG_C Cuts at: 2317 2507 5069 5565 2317 Size: 190 2562 496 2618 Fragments arranged by size: 2618 2562 496 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2160 2453 1163 Size: 573 424 293 4576 Fragments arranged by size: 4576 573 424 293 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2096 2409 3133 3927 4012 5083 2012 Size: 84 313 724 794 85 1071 2795 Fragments arranged by size: 2795 1071 794 724 313 85 84 BsmI GAATG_Cn' Cuts at: 3144 3377 3470 3144 Size: 233 93 5540 Fragments arranged by size: 5540 233 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2096 2409 2677 3005 3133 3185 Size: 399 222 84 313 268 328 128 52 Cuts at: 3185 3927 4012 5083 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 313 268 222 128 85 84 52 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2393 3221 844 Size: 1549 828 3489 Fragments arranged by size: 3489 1549 828 BsrBI CCG'CTC Cuts at: 1394 1823 3753 1394 Size: 429 1930 3507 Fragments arranged by size: 3507 1930 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2472 4361 4535 65 Size: 1183 1224 1889 174 1396 Fragments arranged by size: 1889 1396 1224 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4510 4697 1700 Size: 110 2700 187 2869 Fragments arranged by size: 2869 2700 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5866 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 3926 4938 3926 Size: 1012 4854 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5866 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstYI r'GATC_y Cuts at: 1972 2034 3045 3196 3563 3948 3965 5417 5503 Size: 62 1011 151 367 385 17 1452 86 Cuts at: 5503 5515 5861 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 151 86 62 17 12 BstZ17I GTA'TAC Cuts at: 2211 2211 Size: 5866 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5314 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 3382 4176 4196 3382 Size: 794 20 5052 Fragments arranged by size: 5052 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2224 2287 2571 Size: 54 1425 50 275 18 394 63 284 Cuts at: 2571 2890 3106 3313 4202 5831 8 Size: 319 216 207 889 1629 43 Fragments arranged by size: 1629 1425 889 394 319 284 275 216 207 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2189 2625 2993 3796 1353 Size: 731 105 436 368 803 3423 Fragments arranged by size: 3423 803 731 436 368 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2094 3131 2094 Size: 1037 4829 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2768 2811 2861 3929 4526 5313 5581 Size: 393 1136 43 50 1068 597 787 268 Cuts at: 5581 1239 Size: 1524 Fragments arranged by size: 1524 1136 1068 787 597 393 268 50 43 EcoO109I rG'GnC_Cy Cuts at: 2673 2674 2673 Size: 1 5865 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 3036 5013 1678 Size: 1358 1977 2531 Fragments arranged by size: 2531 1977 1358 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4507 4539 4581 4613 1373 Size: 32 135 32 2935 32 42 32 2626 Fragments arranged by size: 2935 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3210 3456 669 Size: 988 281 1272 246 3079 Fragments arranged by size: 3079 1272 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2549 2809 4980 5164 1305 Size: 1244 260 2171 184 2007 Fragments arranged by size: 2171 2007 1244 260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2226 2289 3108 10 Size: 54 1425 343 394 63 819 2768 Fragments arranged by size: 2768 1425 819 394 343 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2573 3824 4183 4342 518 Size: 648 1407 1251 359 159 2042 Fragments arranged by size: 2042 1407 1251 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2104 2870 2914 3282 3959 5107 5352 1606 Size: 498 766 44 368 677 1148 245 2120 Fragments arranged by size: 2120 1148 766 677 498 368 245 44 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5314 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NsiI A_TGCA'T Cuts at: 2570 2570 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 2987 3224 4769 1132 Size: 97 1758 237 1545 2229 Fragments arranged by size: 2229 1758 1545 237 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5866 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 2531 1108 Size: 1423 4443 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 2406 3935 3967 5448 5480 2374 Size: 32 1529 32 1481 32 2760 Fragments arranged by size: 2760 1529 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PspOMI G'GGCC_C Cuts at: 2673 2673 Size: 5866 PstI C_TGCA'G Cuts at: 830 2475 3218 830 Size: 1645 743 3478 Fragments arranged by size: 3478 1645 743 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 PvuII CAG'CTG Cuts at: 2104 2104 Size: 5866 SacI G_AGCT'C Cuts at: 2096 3133 2096 Size: 1037 4829 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SapI GCTCTTCn'nnn_ Cuts at: 2084 2993 2084 Size: 909 4957 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 2058 4114 5826 2058 Size: 2056 1712 2098 Fragments arranged by size: 2098 2056 1712 SfcI C'TryA_G Cuts at: 826 1045 2471 3214 4349 5030 5221 826 Size: 219 1426 743 1135 681 191 1471 Fragments arranged by size: 1471 1426 1135 743 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3970 4871 5133 820 Size: 197 926 2027 901 262 1553 Fragments arranged by size: 2027 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 2987 3224 1132 Size: 1855 237 3774 Fragments arranged by size: 3774 1855 237 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 2133 1517 Size: 616 5250 StyI C'CwwG_G Cuts at: 513 1065 3305 513 Size: 552 2240 3074 Fragments arranged by size: 3074 2240 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3893 3910 4063 4248 4693 325 Size: 1255 2313 17 153 185 445 1498 Fragments arranged by size: 2313 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2056 4112 5824 Size: 274 80 33 51 1443 79 2056 1712 Cuts at: 5824 96 Size: 138 Fragments arranged by size: 2056 1712 1443 274 138 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2641 2641 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI