Restriction map for
pFN21AB5317
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BanII BbeI
BbrPI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AflIII 677 ACATGT A^C[GA][CT]GT
Aor51HI 938 AGCGCT AGC^GCT
AspHI 1162 GTGCTC G[AT]GC[AT]^C
AspI 601 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 112 CTCGAG C^[CT]CG[GA]G
BamHI 366 GGATCC G^GATCC
BbvCI 669 GCTGAGG CCTCAGC(-5/-2)
BcgI 46 CGACCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 250 CTAG C^TAG
BlpI 888 GCTGAGC GC^T[ACGT]AGC
BmyI 1162 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 888 GCTGAGC GC^T[ACGT]AGC
BsaI 292 GAGACC GGTCTC(1/5)
BsaWI 460 TCCGGT [AT]^CCGG[AT]
BsgI 751 GTGCAG GTGCAG(16/14)
BsiHKAI 1162 GTGCTC G[AT]GC[AT]^C
BsmAI 292 GAGAC GTCTC(1/5)
BsmFI 770 GGGAC GGGAC(10/14)
BsoBI 112 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1162 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 677 ACATGT A^CATGT
BspMI 1076 ACCTGC ACCTGC(4/8)
BstAPI 615 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 987 CCAGACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 888 GCTGAGC GC^T[ACGT]AGC
CfoI 939 GCGC GCG^C
DraI 1155 TTTAAA TTT^AAA
DsaI 173 CCATGG C^C[GA][CT]GG
Eco47III 938 AGCGCT AGC^GCT
EcoNI 609 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 173 CCATGG C^C[AT][AT]GG
FauI 764 CCCGC CCCGC(4/6)
HaeII 938 AGCGCT [GA]GCGC^[CT]
HhaI 939 GCGC GCG^C
HinP1I 939 GCGC G^CGC
HphI 15 GGTGA GGTGA(8/7)
MaeI 250 CTAG C^TAG
MseI 1156 TTAA T^TAA
MspA1I 757 CAGCTG C[AC]G^C[GT]G
NcoI 173 CCATGG C^CATGG
NspI 677 ACATGT [GA]CATG^[CT]
PaeR7I 112 CTCGAG C^TCGAG
PflFI 601 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 757 CAGCTG CAG^CTG
SbfI 612 CCTGCAGG CCTGCA^GG
SexAI 710 ACCAGGT A^CC[AT]GGT
Sse8387I 612 CCTGCAGG CCTGCA^GG
StyI 173 CCATGG C^C[AT][AT]GG
TfiI 457 GATTC G^A[AT]TC
Tru9I 1156 TTAA T^TAA
Tsp45I 731 GTGAC ^GT[GC]AC
Tth111I 601 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 81 CCAGTGTTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 112 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 141 GAATTT [GA]^AATT[CT]
193 GAATTT
AocI 541 CCTGAGG CC^T[ACGT]AGG
1001 CCTCAGG
ApoI 141 GAATTT [GA]^AATT[CT]
193 GAATTT
BanI 721 GGTGCC G^G[CT][GA]CC
1128 GGTGCC
BbsI 355 GAAGAC GAAGAC(2/6)
1072 GAAGAC
BcnI 66 CCCGG CC^[GC]GG
768 CCGGG
BpmI 360 CTGGAG CTGGAG(16/14)
778 CTGGAG
BpuAI 355 GAAGAC GAAGAC(2/6)
1072 GAAGAC
BsaJI 173 CCATGG C^C[ACGT][ACGT]GG
593 CCCAGG
BstNI 594 CCAGG CC^[AT]GG
711 CCAGG
Bsu36I 541 CCTGAGG CC^T[ACGT]AGG
1001 CCTCAGG
Eco81I 541 CCTGAGG CC^T[ACGT]AGG
1001 CCTCAGG
EcoRII 594 CCAGG ^CC[AT]GG
711 CCAGG
HaeIII 6 GGCC GG^CC
762 GGCC
MaeIII 270 GTTAC ^GT[ACGT]AC
731 GTGAC
MvaI 594 CCAGG CC^[AT]GG
711 CCAGG
NciI 66 CCCGG CC^[GC]GG
768 CCGGG
PleI 601 GACTC GAGTC(4/5)
18 GAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbvI BseRI BsiYI BslI BsrI
BssKI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
Fnu4HI FokI HapII HinfI HpaII
ItaI Ksp632I MboI MboII MflI
MnlI MspI MwoI NdeII NlaIII
NlaIV PpuMI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI Tsp509I TspRI TthHB8I XhoII