Restriction map for
pFN21AE1487
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tsp45I TspRI
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 40 GAATTC [GA]^AATT[CT]
AfaI 417 GTAC GT^AC
Alw44I 112 GTGCAC G^TGCAC
ApaI 195 GGGCCC GGGCC^C
ApaLI 112 GTGCAC G^TGCAC
ApoI 40 GAATTC [GA]^AATT[CT]
AspI 166 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 144 CCCGAG C^[CT]CG[GA]G
BanII 195 GGGCCC G[GA]GC[CT]^C
BcnI 199 CCCGG CC^[GC]GG
BglI 480 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 349 GTGCAG GTGCAG(16/14)
BsoBI 144 CCCGAG C^[CT]CG[GA]G
BspHI 446 TCATGA T^CATGA
BstF5I 249 CATCC GGATG(2/0)
DsaI 308 CCGTGG C^C[GA][CT]GG
EcoRI 40 GAATTC G^AATTC
FokI 249 CATCC GGATG(9/13)
HaeII 156 GGCGCT [GA]GCGC^[CT]
HapII 200 CCGG C^CGG
HinfI 450 GAATC G^A[ACGT]TC
HpaII 200 CCGG C^CGG
MspA1I 406 CCGCTG C[AC]G^C[GT]G
MspI 200 CCGG C^CGG
NciI 199 CCCGG CC^[GC]GG
PflFI 166 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 186 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 160 CTGCAG CTGCA^G
RcaI 446 TCATGA T^CATGA
RsaI 417 GTAC GT^AC
SfaNI 24 GATGC GCATC(5/9)
SfcI 160 CTGCAG C^T[GA][CT]AG
SmlI 424 CTCAAG C^T[CT][GA]AG
TaqI 377 TCGA T^CGA
TfiI 450 GAATC G^A[AT]TC
Tsp509I 41 AATT ^AATT
Tth111I 166 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 377 TCGA T^CGA
Van91I 186 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 182 CCAGCCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AspHI 112 GTGCAC G[AT]GC[AT]^C
435 GAGCAC
BpmI 340 CTGGAG CTGGAG(16/14)
391 CTGGAG
BsiHKAI 112 GTGCAC G[AT]GC[AT]^C
435 GAGCAC
BsrBI 147 GAGCGG CCGCTC(-3/-3)
333 GAGCGG
DdeI 55 CTGAG C^T[ACGT]AG
433 CTGAG
DpnII 93 GATC ^GATC
105 GATC
HgaI 300 GACGC GACGC(5/10)
129 GCGTC
MboI 93 GATC ^GATC
105 GATC
MboII 21 GAAGA GAAGA(8/7)
102 GAAGA
MslI 66 CATGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
249 CATCCTGGTG
NdeII 93 GATC ^GATC
105 GATC
NlaIII 66 CATG CATG^
447 CATG
PpuMI 8 GGGACCT [GA]G^G[AT]CC[CT]
170 GGGTCCC
Sau3AI 93 GATC ^GATC
105 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DraII EcoO109I EcoRII FauI
Fnu4HI HaeIII HhaI HinP1I HphI
ItaI MnlI MvaI MwoI NlaIV
Sau96I ScrFI TseI