Restriction map for
pFN21AE1487
(Linear) (Six-base) MAPSORT of: KIEE1487.seq Check: 9127 from: 1 to: 495
KIEE1487.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 340 495
Size: 103 237 155
Fragments arranged by size:
237 155 103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 229 495
Size: 229 266
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 346 378 495
Size: 346 32 117
Fragments arranged by size:
346 117 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 192 481 495
Size: 132 60 289 14
Fragments arranged by size:
289 132 60 14
ApaI G_GGCC'C
Cuts at: 0 199 495
Size: 199 296
ApaLI G'TGCA_C
Cuts at: 0 112 495
Size: 112 383
ApoI r'AATT_y
Cuts at: 0 40 495
Size: 40 455
AvaI C'yCGr_G
Cuts at: 0 144 495
Size: 144 351
BanII G_rGCy'C
Cuts at: 0 199 495
Size: 199 296
BglI GCCn_nnn'nGGC
Cuts at: 0 486 495
Size: 486 9
Bme1580I G_kGCm'C
Cuts at: 0 116 199 495
Size: 116 83 296
Fragments arranged by size:
296 116 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 412 495
Size: 361 51 83
Fragments arranged by size:
361 83 51
Bpu10I CC'TnA_GC
Cuts at: 0 55 433 495
Size: 55 378 62
Fragments arranged by size:
378 62 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 495
Size: 409 86
BseYI C'CCAG_C
Cuts at: 0 5 408 495
Size: 5 403 87
Fragments arranged by size:
403 87 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 495
Size: 370 125
BsiHKAI G_wGCw'C
Cuts at: 0 116 439 495
Size: 116 323 56
Fragments arranged by size:
323 116 56
Bsp1286I G_dGCh'C
Cuts at: 0 116 199 439 495
Size: 116 83 240 56
Fragments arranged by size:
240 116 83 56
BspHI T'CATG_A
Cuts at: 0 446 495
Size: 446 49
BsrBI CCG'CTC
Cuts at: 0 149 335 495
Size: 149 186 160
Fragments arranged by size:
186 160 149
BtgI C'CryG_G
Cuts at: 0 308 495
Size: 308 187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 340 361 412 495
Size: 103 237 21 51 83
Fragments arranged by size:
237 103 83 51 21
EcoO109I rG'GnC_Cy
Cuts at: 0 9 171 189 196 495
Size: 9 162 18 7 299
Fragments arranged by size:
299 162 18 9 7
EcoRI G'AATT_C
Cuts at: 0 40 495
Size: 40 455
HaeII r_GCGC'y
Cuts at: 0 160 495
Size: 160 335
MslI CAynn'nnrTG
Cuts at: 0 70 253 495
Size: 70 183 242
Fragments arranged by size:
242 183 70
MspA1I CmG'CkG
Cuts at: 0 408 495
Size: 408 87
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 495
Size: 192 303
PpuMI rG'GwC_Cy
Cuts at: 0 9 171 495
Size: 9 162 324
Fragments arranged by size:
324 162 9
PspOMI G'GGCC_C
Cuts at: 0 195 495
Size: 195 300
PstI C_TGCA'G
Cuts at: 0 164 495
Size: 164 331
SanDI GG'GwC_CC
Cuts at: 0 171 495
Size: 171 324
SfcI C'TryA_G
Cuts at: 0 160 495
Size: 160 335
SmlI C'TyrA_G
Cuts at: 0 424 495
Size: 424 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 495
Size: 112 383
Tth111I GACn'n_nGTC
Cuts at: 0 169 495
Size: 169 326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 495
Size: 189 306
Enzymes that do cut:
AcuI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BanII BglI Bme1580I BpmI Bpu10I BpuEI BseYI BsgI
BsiHKAI Bsp1286I BspHI BsrBI BtgI Eco57MI EcoO109I EcoRI
HaeII MslI MspA1I PflMI PpuMI PspOMI PstI SanDI
SfcI SmlI TaqII Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI TstI XbaI XhoI XmaI XmnI ZraI