################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI AlwI AlwNI AocI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AvrII BalI BamHI BbsI BciVI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BseAI BseRI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoRI EcoT14I EcoT22I FseI HindIII HpaI KpnI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI ScaI SexAI SfuI SmaI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 363 GACGTC GACGT^C AccI 190 GTCGAC GT^[AC][GT]AC AfaI 915 GTAC GT^AC Alw44I 580 GTGCAC G^TGCAC Aor51HI 545 AGCGCT AGC^GCT ApaI 9 GGGCCC GGGCC^C ApaLI 580 GTGCAC G^TGCAC AscI 345 GGCGCGCC GG^CGCGCC AviII 446 TGCGCA TGC^GCA BanI 493 GGCGCC G^G[CT][GA]CC BbeI 493 GGCGCC GGCGC^C BbrPI 102 CACGTG CAC^GTG BbvCI 525 GCTGAGG CCTCAGC(-5/-2) BcgI 182 GCATGGCGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 887 TGATCA T^GATCA BsaAI 102 CACGTG [CT]AC^GT[GA] BsaWI 896 ACCGGA [AT]^CCGG[AT] BsmBI 626 CGTCTC CGTCTC(1/5) BssSI 289 CTCGTG CACGAG(-5/-1) BstEII 69 GGTGACC G^GT[ACGT]ACC BstPI 69 GGTGACC G^GT[ACGT]ACC BstXI 563 CCAAGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 714 CCGCGG C^C[GA][CT]GG EarI 535 CTCTTC CTCTTC(1/4) Eco47III 545 AGCGCT AGC^GCT EcoO65I 69 GGTGACC G^GT[ACGT]ACC EcoRV 652 GATATC GAT^ATC FauI 257 CCCGC CCCGC(4/6) FbaI 887 TGATCA T^GATCA FspI 446 TGCGCA TGC^GCA HgaI 517 GACGC GACGC(5/10) HincII 190 GTCGAC GT[CT]^[GA]AC HindII 190 GTCGAC GT[CT]^[GA]AC KasI 493 GGCGCC G^GCGCC Ksp632I 535 CTCTTC CTCTTC(1/4) KspI 714 CCGCGG CCGC^GG MboII 536 TCTTC GAAGA(8/7) NarI 493 GGCGCC GG^CGCC NotI 149 GCGGCCGC GC^GGCCGC PleI 697 GAGTC GAGTC(4/5) PmaCI 102 CACGTG CAC^GTG PmlI 102 CACGTG CAC^GTG PstI 751 CTGCAG CTGCA^G RsaI 915 GTAC GT^AC SacII 714 CCGCGG CCGC^GG SalI 190 GTCGAC G^TCGAC SapI 534 GCTCTTC GCTCTTC(1/4) SbfI 750 CCTGCAGG CCTGCA^GG SfaNI 661 GATGC GCATC(5/9) SfcI 751 CTGCAG C^T[GA][CT]AG SfoI 493 GGCGCC GGC^GCC SmlI 226 CTCAAG C^T[CT][GA]AG Sse8387I 750 CCTGCAGG CCTGCA^GG TfiI 789 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 103 ACGTGT A^C[GA][CT]GT 623 ACACGT AspHI 280 GAGCAC G[AT]GC[AT]^C 580 GTGCAC BanII 9 GGGCCC G[GA]GC[CT]^C 174 GGGCTC BcnI 13 CCCGG CC^[GC]GG 145 CCCGG BsiHKAI 280 GAGCAC G[AT]GC[AT]^C 580 GTGCAC BsmAI 627 GTCTC GTCTC(1/5) 699 GTCTC BsmFI 683 GGGAC GGGAC(10/14) 107 GTCCC BspMI 470 ACCTGC ACCTGC(4/8) 753 GCAGGT BstF5I 459 CATCC GGATG(2/0) 600 CATCC DraII 8 GGGGCCC [GA]G^G[ACGT]CC[CT] 9 GGGCCCC EcoO109I 8 GGGGCCC [GA]G^G[ACGT]CC[CT] 9 GGGCCCC FokI 459 CATCC GGATG(9/13) 600 CATCC HinfI 697 GAGTC G^A[ACGT]TC 789 GATTC MaeIII 70 GTGAC ^GT[ACGT]AC 644 GTGAC MslI 261 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 840 CATCGTGGTG NciI 13 CCCGG CC^[GC]GG 145 CCCGG SfiI 142 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 712 GGCCGCGGCGGCC SgrAI 489 CGCCGGCG C[GA]^CCGG[CT]G 639 CGCCGGTG Tsp45I 70 GTGAC ^GT[GC]AC 644 GTGAC TspRI 160 CAGTG CA[GC]TG[ACGT][ACGT]^ 352 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AcyI AluI AvaI BbvI BglI BmyI BsaHI BsaJI BsgI BsiEI BsiYI BslI BsoBI Bsp1286I BsrFI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EagI EclXI Eco52I EcoRII Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NdeII NgoMIV NlaIII NlaIV Sau3AI Sau96I ScrFI TaqI TseI TthHB8I