(Linear) (Six-base) MAPSORT of: KIEE4317.seq Check: 6663 from: 1 to: 969 KIEE4317.orf With 182 enzymes: SgfI * March 22, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 744 969 Size: 744 225 AatII G_ACGT'C Cuts at: 0 367 969 Size: 367 602 AccI GT'mk_AC Cuts at: 0 191 969 Size: 191 778 AfeI AGC'GCT Cuts at: 0 547 969 Size: 547 422 AflIII A'CryG_T Cuts at: 0 103 623 969 Size: 103 520 346 Fragments arranged by size: 520 346 103 AleI CACnn'nnGTG Cuts at: 0 265 969 Size: 265 704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 283 969 Size: 283 686 ApaI G_GGCC'C Cuts at: 0 13 969 Size: 13 956 ApaLI G'TGCA_C Cuts at: 0 580 969 Size: 580 389 AscI GG'CGCG_CC Cuts at: 0 346 969 Size: 346 623 AvaI C'yCGr_G Cuts at: 0 5 177 328 969 Size: 5 172 151 641 Fragments arranged by size: 641 172 151 5 BanI G'GyrC_C Cuts at: 0 493 969 Size: 493 476 BanII G_rGCy'C Cuts at: 0 13 178 969 Size: 13 165 791 Fragments arranged by size: 791 165 13 BbeI G_GCGC'C Cuts at: 0 497 969 Size: 497 472 BbvCI CC'TCA_GC Cuts at: 0 526 969 Size: 526 443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 171 205 969 Size: 171 34 764 Fragments arranged by size: 764 171 34 BclI T'GATC_A Cuts at: 0 887 969 Size: 887 82 BglI GCCn_nnn'nGGC Cuts at: 0 140 149 719 874 969 Size: 140 9 570 155 95 Fragments arranged by size: 570 155 140 95 9 Bme1580I G_kGCm'C Cuts at: 0 13 584 969 Size: 13 571 385 Fragments arranged by size: 571 385 13 BmgBI CAC'GTC Cuts at: 0 626 648 969 Size: 626 22 321 Fragments arranged by size: 626 321 22 BmrI ACTGGGnnnn_n' Cuts at: 0 949 969 Size: 949 20 Bpu10I CC'TnA_GC Cuts at: 0 421 526 969 Size: 421 105 443 Fragments arranged by size: 443 421 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 969 Size: 211 758 BsaAI yAC'GTr Cuts at: 0 104 969 Size: 104 865 BsaHI Gr'CG_yC Cuts at: 0 364 494 518 969 Size: 364 130 24 451 Fragments arranged by size: 451 364 130 24 BsaWI w'CCGG_w Cuts at: 0 896 969 Size: 896 73 BseYI C'CCAG_C Cuts at: 0 171 969 Size: 171 798 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 150 541 969 Size: 84 66 391 428 Fragments arranged by size: 428 391 84 66 BsiEI CG_ry'CG Cuts at: 0 86 153 191 274 578 969 Size: 86 67 38 83 304 391 Fragments arranged by size: 391 304 86 83 67 38 BsiHKAI G_wGCw'C Cuts at: 0 284 584 969 Size: 284 300 385 Fragments arranged by size: 385 300 284 BsmBI CGTCTCn'nnnn_ Cuts at: 0 632 969 Size: 632 337 Bsp1286I G_dGCh'C Cuts at: 0 13 178 284 584 969 Size: 13 165 106 300 385 Fragments arranged by size: 385 300 165 106 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 479 744 969 Size: 479 265 225 Fragments arranged by size: 479 265 225 BsrFI r'CCGG_y Cuts at: 0 73 490 640 823 868 969 Size: 73 417 150 183 45 101 Fragments arranged by size: 417 183 150 101 73 45 BssHII G'CGCG_C Cuts at: 0 167 232 346 969 Size: 167 65 114 623 Fragments arranged by size: 623 167 114 65 BssSI C'ACGA_G Cuts at: 0 289 969 Size: 289 680 BstEII G'GTnAC_C Cuts at: 0 69 969 Size: 69 900 BstXI CCAn_nnnn'nTGG Cuts at: 0 570 969 Size: 570 399 BtgI C'CryG_G Cuts at: 0 714 969 Size: 714 255 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 449 674 816 969 Size: 449 225 142 153 Fragments arranged by size: 449 225 153 142 EaeI y'GGCC_r Cuts at: 0 75 83 150 271 575 875 969 Size: 75 8 67 121 304 300 94 Fragments arranged by size: 304 300 121 94 75 67 8 EagI C'GGCC_G Cuts at: 0 83 150 271 575 969 Size: 83 67 121 304 394 Fragments arranged by size: 394 304 121 83 67 EarI CTCTTCn'nnn_ Cuts at: 0 541 969 Size: 541 428 EcoO109I rG'GnC_Cy Cuts at: 0 9 10 969 Size: 9 1 959 Fragments arranged by size: 959 9 1 EcoRV GAT'ATC Cuts at: 0 654 969 Size: 654 315 FspI TGC'GCA Cuts at: 0 448 969 Size: 448 521 FspAI rTGC'GCAy Cuts at: 0 448 969 Size: 448 521 HaeII r_GCGC'y Cuts at: 0 245 497 549 816 969 Size: 245 252 52 267 153 Fragments arranged by size: 267 252 245 153 52 HincII GTy'rAC Cuts at: 0 192 969 Size: 192 777 KasI G'GCGC_C Cuts at: 0 493 969 Size: 493 476 MslI CAynn'nnrTG Cuts at: 0 265 844 969 Size: 265 579 125 Fragments arranged by size: 579 265 125 MspA1I CmG'CkG Cuts at: 0 33 45 155 716 969 Size: 33 12 110 561 253 Fragments arranged by size: 561 253 110 33 12 NaeI GCC'GGC Cuts at: 0 492 825 870 969 Size: 492 333 45 99 Fragments arranged by size: 492 333 99 45 NarI GG'CG_CC Cuts at: 0 494 969 Size: 494 475 NgoMIV G'CCGG_C Cuts at: 0 490 823 868 969 Size: 490 333 45 101 Fragments arranged by size: 490 333 101 45 NotI GC'GGCC_GC Cuts at: 0 150 969 Size: 150 819 PmlI CAC'GTG Cuts at: 0 104 969 Size: 104 865 PspOMI G'GGCC_C Cuts at: 0 9 969 Size: 9 960 PstI C_TGCA'G Cuts at: 0 755 969 Size: 755 214 SacII CC_GC'GG Cuts at: 0 717 969 Size: 717 252 SalI G'TCGA_C Cuts at: 0 190 969 Size: 190 779 SapI GCTCTTCn'nnn_ Cuts at: 0 541 969 Size: 541 428 SbfI CC_TGCA'GG Cuts at: 0 755 969 Size: 755 214 SfcI C'TryA_G Cuts at: 0 751 969 Size: 751 218 SfiI GGCCn_nnn'nGGCC Cuts at: 0 149 719 969 Size: 149 570 250 Fragments arranged by size: 570 250 149 SfoI GGC'GCC Cuts at: 0 495 969 Size: 495 474 SgrAI Cr'CCGG_yG Cuts at: 0 490 640 969 Size: 490 150 329 Fragments arranged by size: 490 329 150 SmlI C'TyrA_G Cuts at: 0 226 969 Size: 226 743 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 466 969 Size: 466 503 TatI w'GTAC_w Cuts at: 0 914 969 Size: 914 55 ZraI GAC'GTC Cuts at: 0 365 969 Size: 365 604 Enzymes that do cut: AarI AatII AccI AfeI AflIII AleI AlfI ApaI ApaLI AscI AvaI BanI BanII BbeI BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BssSI BstEII BstXI BtgI BtgZI EaeI EagI EarI EcoO109I EcoRV FspI FspAI HaeII HincII KasI MslI MspA1I NaeI NarI NgoMIV NotI PmlI PspOMI PstI SacII SalI SapI SbfI SfcI SfiI SfoI SgrAI SmlI TaqII TatI ZraI Enzymes that do not cut: Acc65I AclI AcuI AflII AgeI AhdI AloI AlwNI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BfrBI BglII BlpI BmtI BplI BpmI BsaI BsaBI BsaXI BseRI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FseI Hin4I HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SanDI ScaI SexAI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI