################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflIII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BglI BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflII 454 CTTAAG C^TTAAG AhdI 9 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 13 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 9 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 29 TGCGCA TGC^GCA BcnI 155 CCCGG CC^[GC]GG BfaI 166 CTAG C^TAG BfrI 454 CTTAAG C^TTAAG BglII 404 AGATCT A^GATCT BlpI 699 GCTGAGC GC^T[ACGT]AGC Bpu1102I 699 GCTGAGC GC^T[ACGT]AGC BsaWI 311 TCCGGT [AT]^CCGG[AT] BseRI 459 GAGGAG GAGGAG(10/8) BsgI 480 CTGCAC GTGCAG(16/14) BsmAI 208 GAGAC GTCTC(1/5) BsmFI 153 GTCCC GGGAC(10/14) BsmI 449 GCATTC GAATGC(1/-1) Bsp1407I 321 TGTACA T^GTACA BspMI 647 ACCTGC ACCTGC(4/8) BsrGI 321 TGTACA T^GTACA BstAPI 12 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 225 CCACCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 699 GCTGAGC GC^T[ACGT]AGC CfoI 30 GCGC GCG^C Eam1105I 9 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 177 GAAGAG CTCTTC(1/4) EcoRV 130 GATATC GAT^ATC FspI 29 TGCGCA TGC^GCA HgaI 9 GACGC GACGC(5/10) HhaI 30 GCGC GCG^C HinP1I 30 GCGC G^CGC HinfI 159 GAATC G^A[ACGT]TC Ksp632I 177 GAAGAG CTCTTC(1/4) MaeI 166 CTAG C^TAG MaeII 34 ACGT A^CGT MseI 455 TTAA T^TAA MspA1I 680 CCGCTG C[AC]G^C[GT]G NciI 155 CCCGG CC^[GC]GG NheI 165 GCTAGC G^CTAGC PflMI 53 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SspBI 321 TGTACA T^GTACA TaqI 123 TCGA T^CGA TfiI 159 GAATC G^A[AT]TC Tru9I 455 TTAA T^TAA Tsp509I 105 AATT ^AATT TthHB8I 123 TCGA T^CGA Van91I 53 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AocI 240 CCTGAGG CC^T[ACGT]AGG 669 CCTCAGG BbvI 497 GCAGC GCAGC(8/12) 27 GCTGC BpmI 537 CTCCAG CTGGAG(16/14) 60 CTGGAG BsrI 432 ACTGG ACTGG(1/-1) 366 CCAGT Bsu36I 240 CCTGAGG CC^T[ACGT]AGG 669 CCTCAGG Cac8I 165 GCTAGC GC[ACGT]^[ACGT]GC 394 GCAAGC Eco81I 240 CCTGAGG CC^T[ACGT]AGG 669 CCTCAGG MaeIII 530 GTGAC ^GT[ACGT]AC 620 GTGAC MslI 33 CACGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 226 CACCATGATG PpuMI 236 GGGTCCT [GA]G^G[AT]CC[CT] 509 AGGACCT SfaNI 3 GATGC GCATC(5/9) 664 GATGC SmlI 283 CTCAAG C^T[CT][GA]AG 454 CTTAAG TseI 27 GCTGC G^C[AT]GC 497 GCAGC Tsp45I 530 GTGAC ^GT[GC]AC 620 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BsaJI BsiYI BslI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TspRI XhoII