################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MboII MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 51 CGCG CG^CG AcsI 451 GAATTC [GA]^AATT[CT] AflIII 347 ACATGT A^C[GA][CT]GT AlwNI 4 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 451 GAATTC [GA]^AATT[CT] AvaII 129 GGACC G^G[AT]CC AviII 60 TGCGCA TGC^GCA BcgI 548 GCATCAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 140 TGATCA T^GATCA BcnI 494 CCGGG CC^[GC]GG BglI 477 GCCCAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 282 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 233 CTCCTC GAGGAG(10/8) BspLU11I 347 ACATGT A^CATGT BstF5I 83 CATCC GGATG(2/0) BstUI 51 CGCG CG^CG BstYI 287 AGATCC [GA]^GATC[CT] CfoI 61 GCGC GCG^C DraII 128 AGGACCC [GA]G^G[ACGT]CC[CT] DrdI 166 GACCCCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 9 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 128 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 451 GAATTC G^AATTC FbaI 140 TGATCA T^GATCA FokI 83 CATCC GGATG(9/13) FspI 60 TGCGCA TGC^GCA HapII 494 CCGG C^CGG HhaI 61 GCGC GCG^C HinP1I 61 GCGC G^CGC HinfI 153 GACTC G^A[ACGT]TC HpaII 494 CCGG C^CGG MaeIII 190 GTGAC ^GT[ACGT]AC MamI 282 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 287 AGATCC [GA]^GATC[CT] MseI 352 TTAA T^TAA MslI 328 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 312 CAGCTG C[AC]G^C[GT]G MspI 494 CCGG C^CGG MvnI 51 CGCG CG^CG NciI 494 CCGGG CC^[GC]GG NspI 347 ACATGT [GA]CATG^[CT] PleI 153 GACTC GAGTC(4/5) PpuMI 128 AGGACCC [GA]G^G[AT]CC[CT] PvuII 312 CAGCTG CAG^CTG Tru9I 352 TTAA T^TAA Tsp45I 190 GTGAC ^GT[GC]AC XhoII 287 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 317 GTAC GT^AC 389 GTAC AlwI 288 GATCC GGATC(4/5) 281 GGATC BanII 367 GAGCCC G[GA]GC[CT]^C 379 GAGCCC BmyI 367 GAGCCC G[AGT]GC[ACT]^C 379 GAGCCC BpmI 157 CTGGAG CTGGAG(16/14) 469 CTGGAG BsiYI 9 CCTGATGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 150 CCTGACTCTGG BslI 9 CCTGATGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 150 CCTGACTCTGG Bsp1286I 367 GAGCCC G[AGT]GC[ACT]^C 379 GAGCCC Cfr13I 54 GGCCC G^G[ACGT]CC 129 GGACC HaeIII 54 GGCC GG^CC 274 GGCC HgaI 515 GCGTC GACGC(5/10) 537 GCGTC HphI 138 GGTGA GGTGA(8/7) 488 TCACC NlaIII 293 CATG CATG^ 348 CATG RsaI 317 GTAC GT^AC 389 GTAC Sau96I 54 GGCCC G^G[ACGT]CC 129 GGACC SfaNI 12 GATGC GCATC(5/9) 548 GCATC Tsp509I 354 AATT ^AATT 452 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI BbvI BsaJI BsmFI BsrI BssKI BstNI Cac8I DdeI DpnII EcoRII Fnu4HI ItaI MboI MnlI MvaI MwoI NdeII NlaIV Sau3AI ScrFI TseI TspRI