Restriction map for
pFN21AE6216
(Circular) (Six-base) MAPSORT of: pFN21AE6216.seq Check: 7160 from: 1 to: 5284
pFN21AE6216 5284 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3094 278
Size: 53 83 186 936 1558 2468
Fragments arranged by size:
2468 1558 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2627 1937
Size: 690 4594
Acc65I G'GTAC_C
Cuts at: 2605 2605
Size: 5284
AclI AA'CG_TT
Cuts at: 1563 3411 3784 1563
Size: 1848 373 3063
Fragments arranged by size:
3063 1848 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2264 3347 4731 4999 1632
Size: 632 1083 1384 268 1917
Fragments arranged by size:
1917 1384 1083 632 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5087
AflIII A'CryG_T
Cuts at: 1203 2352 4183 5160 1203
Size: 1149 1831 977 1327
Fragments arranged by size:
1831 1327 1149 977
AgeI A'CCGG_T
Cuts at: 4115 4115
Size: 5284
AhdI GACnn_n'nnGTC
Cuts at: 4013 4013
Size: 5284
AleI CACnn'nnGTG
Cuts at: 1166 2337 1166
Size: 1171 4113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5284
AlwNI CAG_nnn'CTG
Cuts at: 2014 3027 4599 2014
Size: 1013 1572 2699
Fragments arranged by size:
2699 1572 1013
ApaLI G'TGCA_C
Cuts at: 3341 4497 3341
Size: 1156 4128
ApoI r'AATT_y
Cuts at: 1493 2456 2593 2819 2971 5080 1493
Size: 963 137 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 3838 160
Size: 872 2806 1606
Fragments arranged by size:
2806 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5284
AvaI C'yCGr_G
Cuts at: 1870 1943 2609 1870
Size: 73 666 4545
Fragments arranged by size:
4545 666 73
BamHI G'GATC_C
Cuts at: 2614 2981 2614
Size: 367 4917
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2605 4060 618
Size: 325 217 627 818 1455 1842
Fragments arranged by size:
1842 1455 818 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2376 2388 2603 1391
Size: 985 12 215 4072
Fragments arranged by size:
4072 985 215 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2542 2576 3475 3509 1903
Size: 34 50 34 521 34 899 34 3678
Fragments arranged by size:
3678 899 521 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3176 4386 3176
Size: 1210 4074
BclI T'GATC_A
Cuts at: 1548 2145 1548
Size: 597 4687
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2488 3895 136
Size: 107 122 71 631 605 816 1407 1525
Fragments arranged by size:
1525 1407 816 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5279 5279
Size: 5284
BlpI GC'TnA_GC
Cuts at: 2701 2701
Size: 5284
Bme1580I G_kGCm'C
Cuts at: 1790 3345 4501 1790
Size: 1555 1156 2573
Fragments arranged by size:
2573 1555 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5284
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2376 2478 3015
Size: 416 53 83 399 24 959 102 537
Cuts at: 3015 3973 442
Size: 958 1753
Fragments arranged by size:
1753 959 958 537 416 399 102 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5284
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2256 2256
Size: 5284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2495 3944 1239
Size: 944 312 1449 2579
Fragments arranged by size:
2579 1449 944 312
Bpu10I CC'TnA_GC
Cuts at: 2525 2525
Size: 5284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3409 4274 4572 3409
Size: 865 298 4121
Fragments arranged by size:
4121 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3947 882
Size: 617 2448 2219
Fragments arranged by size:
2448 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3075 493
Size: 711 1871 2702
Fragments arranged by size:
2702 1871 711
BsaBI GATnn'nnATC
Cuts at: 2291 5278 2291
Size: 2987 2297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3091 3473 4108 275
Size: 53 83 186 936 1558 382 635 1451
Fragments arranged by size:
1558 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3717 4115 4389 4536 4947 3717
Size: 398 274 147 411 4054
Fragments arranged by size:
4054 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5059
Fragments arranged by size:
5059 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2229 1186
Size: 1043 4241
BseYI C'CCAG_C
Cuts at: 2091 4487 4983 2091
Size: 2396 496 2392
Fragments arranged by size:
2396 2392 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4711
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2734 3495 4523 1540
Size: 275 186 733 761 1028 2301
Fragments arranged by size:
2301 1028 761 733 275 186
BsiHKAI G_wGCw'C
Cuts at: 3345 3430 4501 3345
Size: 85 1071 4128
Fragments arranged by size:
4128 1071 85
BsmI GAATG_Cn'
Cuts at: 2795 2888 2795
Size: 93 5191
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2376 2388 2603 3345 3430 4501 1391
Size: 399 586 12 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 586 399 215 85 12
BspEI T'CCGG_A
Cuts at: 4947 4947
Size: 5284
BspHI T'CATG_A
Cuts at: 3173 4903 3173
Size: 1730 3554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2639 844
Size: 1795 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3171 1394
Size: 429 1348 3507
Fragments arranged by size:
3507 1348 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3779 3953 65
Size: 1183 2531 174 1396
Fragments arranged by size:
2531 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3928 4115 1700
Size: 110 2118 187 2869
Fragments arranged by size:
2869 2118 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5284
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5284
BssSI C'ACGA_G
Cuts at: 3344 4356 3344
Size: 1012 4272
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5284
BstBI TT'CG_AA
Cuts at: 4088 4088
Size: 5284
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5284
BstYI r'GATC_y
Cuts at: 1972 2292 2614 2981 3366 3383 4835 4921 4933
Size: 320 322 367 385 17 1452 86 12
Cuts at: 4933 5279 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 322 320 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4732
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4545
Fragments arranged by size:
4545 683 56
BtsI GCAGTG_nn'
Cuts at: 2441 2539 2800 3594 3614 2441
Size: 98 261 794 20 4111
Fragments arranged by size:
4111 794 261 98 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5284
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5249
DraI TTT'AAA
Cuts at: 2588 2588
Size: 5284
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2177 4291 809
Size: 507 861 2114 1802
Fragments arranged by size:
2114 1802 861 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2731 3620 5249
Size: 54 1425 50 275 18 901 889 1629
Cuts at: 5249 8
Size: 43
Fragments arranged by size:
1629 1425 901 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2731 1537
Size: 275 919 4090
Fragments arranged by size:
4090 919 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3214 1353
Size: 1861 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3871 4245 4391 387
Size: 1064 138 2282 374 146 1280
Fragments arranged by size:
2282 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2264 2495 3347 3944 4731 4999
Size: 393 551 81 231 852 597 787 268
Cuts at: 4999 1239
Size: 1524
Fragments arranged by size:
1524 852 787 597 551 393 268 231
81
EcoNI CCTnn'n_nnAGG
Cuts at: 2018 2018
Size: 5284
EcoO109I rG'GnC_Cy
Cuts at: 2134 2134
Size: 5284
EcoRI G'AATT_C
Cuts at: 2456 2593 2971 2456
Size: 137 378 4769
Fragments arranged by size:
4769 378 137
FspI TGC'GCA
Cuts at: 2067 3790 2067
Size: 1723 3561
HaeII r_GCGC'y
Cuts at: 1678 4431 1678
Size: 2753 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 3925 3957 3999
Size: 32 135 32 436 32 1885 32 42
Cuts at: 3999 4031 1373
Size: 32 2626
Fragments arranged by size:
2626 1885 436 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2628 2874 669
Size: 988 281 690 246 3079
Fragments arranged by size:
3079 988 690 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5284
HpaI GTT'AAC
Cuts at: 2874 2874
Size: 5284
KpnI G_GTAC'C
Cuts at: 2609 2609
Size: 5284
MfeI C'AATT_G
Cuts at: 2883 2883
Size: 5284
MluI A'CGCG_T
Cuts at: 5160 5160
Size: 5284
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4398 4582 1305
Size: 3093 184 2007
Fragments arranged by size:
3093 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3462
Fragments arranged by size:
3462 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2337 3242 3601 3760 518
Size: 648 1171 905 359 159 2042
Fragments arranged by size:
2042 1171 905 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2319 2700 3377 4525 4770 1606
Size: 713 381 677 1148 245 2120
Fragments arranged by size:
2120 1148 713 677 381 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4732
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5284
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5284
NotI GC'GGCC_GC
Cuts at: 2731 2731
Size: 5284
NspI r_CATG'y
Cuts at: 1132 1229 2356 2642 4187 1132
Size: 97 1127 286 1545 2229
Fragments arranged by size:
2229 1545 1127 286 97
PasI CC'CwG_GG
Cuts at: 1348 2041 1348
Size: 693 4591
PciI A'CATG_T
Cuts at: 2352 4183 2352
Size: 1831 3453
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5284
PfoI T'CCnGG_A
Cuts at: 2185 2185
Size: 5284
PmeI GTTT'AAAC
Cuts at: 2588 2588
Size: 5284
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 4866 4898 3353
Size: 32 1481 32 3739
Fragments arranged by size:
3739 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2134
Size: 5284
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5284
PsiI TTA'TAA
Cuts at: 2854 2854
Size: 5284
PstI C_TGCA'G
Cuts at: 830 2636 830
Size: 1806 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5284
PvuII CAG'CTG
Cuts at: 2319 2319
Size: 5284
SalI G'TCGA_C
Cuts at: 1936 2626 1936
Size: 690 4594
SbfI CC_TGCA'GG
Cuts at: 2636 2636
Size: 5284
ScaI AGT'ACT
Cuts at: 3532 5244 3532
Size: 1712 3572
SfcI C'TryA_G
Cuts at: 826 1045 2632 3767 4448 4639 826
Size: 219 1587 1135 681 191 1471
Fragments arranged by size:
1587 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5284
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5284
SmaI CCC'GGG
Cuts at: 1872 2611 1872
Size: 739 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3388 4289 4551 820
Size: 197 926 1445 901 262 1553
Fragments arranged by size:
1553 1445 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3075 493
Size: 2582 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5284
SphI G_CATG'C
Cuts at: 1132 2642 1132
Size: 1510 3774
SspI AAT'ATT
Cuts at: 5 52 3208 5
Size: 47 3156 2081
Fragments arranged by size:
3156 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5284
StyI C'CwwG_G
Cuts at: 513 1065 2723 513
Size: 552 1658 3074
Fragments arranged by size:
3074 1658 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3311 3328 3481 3666 4111 325
Size: 1255 1731 17 153 185 445 1498
Fragments arranged by size:
1731 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3530 5242 96
Size: 274 80 33 51 1443 1553 1712 138
Fragments arranged by size:
1712 1553 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5244 5276 5244
Size: 32 5252
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5284
XbaI T'CTAG_A
Cuts at: 2620 2620
Size: 5284
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5284
XmaI C'CCGG_G
Cuts at: 1870 2609 1870
Size: 739 4545
XmnI GAAnn'nnTTC
Cuts at: 3413 3413
Size: 5284
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3092 276
Size: 53 83 186 936 1558 2468
Fragments arranged by size:
2468 1558 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI