Restriction map for
pFN21AE4539
(Circular) (Six-base) MAPSORT of: pFN21AE4539.seq Check: 2267 from: 1 to: 6571
pFN21AE4539 6571 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3057 3176 3429 4381
Size: 53 83 186 936 1521 119 253 952
Cuts at: 4381 278
Size: 2468
Fragments arranged by size:
2468 1521 952 936 253 186 119 83
53
AccI GT'mk_AC
Cuts at: 1937 2717 3914 1937
Size: 780 1197 4594
Fragments arranged by size:
4594 1197 780
Acc65I G'GTAC_C
Cuts at: 3321 3892 3321
Size: 571 6000
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2065 2898 3209 3674 4634 6018 6286 1632
Size: 433 833 311 465 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 833 465 433 311 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 3402 3404 5470 6447 1203
Size: 2199 2 2066 977 1327
Fragments arranged by size:
2199 2066 1327 977 2
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 2907 5300 2907
Size: 2393 4178
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2154 1184
Size: 970 5601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2850 2882 2850
Size: 32 6539
AlwNI CAG_nnn'CTG
Cuts at: 2488 3118 3375 3397 4314 5886 2488
Size: 630 257 22 917 1572 3173
Fragments arranged by size:
3173 1572 917 630 257 22
ApaI G_GGCC'C
Cuts at: 2406 3514 2406
Size: 1108 5463
ApaLI G'TGCA_C
Cuts at: 4628 5784 4628
Size: 1156 5415
ApoI r'AATT_y
Cuts at: 1493 3880 4106 4258 6367 1493
Size: 2387 226 152 2109 1697
Fragments arranged by size:
2387 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 2104 2339 2398 2461 2474 2638 2832
Size: 73 161 235 59 63 13 164 194
Cuts at: 2832 2930 3098 3341 3796 3896 1870
Size: 98 168 243 455 100 4545
Fragments arranged by size:
4545 455 243 235 194 168 164 161
100 98 73 63 59 13
AvrII C'CTAG_G
Cuts at: 3814 3814
Size: 6571
BamHI G'GATC_C
Cuts at: 3690 3901 4268 3690
Size: 211 367 5993
Fragments arranged by size:
5993 367 211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3321 3669 3892 5347 618
Size: 325 217 627 1534 348 223 1455 1842
Fragments arranged by size:
1842 1534 1455 627 348 325 223 217
BanII G_rGCy'C
Cuts at: 1391 2337 2406 2473 2557 3081 3146 3356 3514
Size: 946 69 67 84 524 65 210 158
Cuts at: 3514 3890 1391
Size: 376 4072
Fragments arranged by size:
4072 946 524 376 210 158 84 69
67 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2906 928
Size: 971 1007 4593
Fragments arranged by size:
4593 1007 971
BbvCI CC'TCA_GC
Cuts at: 3335 3615 3776 3335
Size: 280 161 6130
Fragments arranged by size:
6130 280 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4762 4796 1903
Size: 34 2825 34 3678
Fragments arranged by size:
3678 2825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4463 5673 4463
Size: 1210 5361
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2603 2678 2999
Size: 107 122 71 631 605 931 75 321
Cuts at: 2999 5182 136
Size: 2183 1525
Fragments arranged by size:
2183 1525 931 631 605 321 122 107
75 71
BglII A'GATC_T
Cuts at: 6566 6566
Size: 6571
BlpI GC'TnA_GC
Cuts at: 2534 3350 3988 2534
Size: 816 638 5117
Fragments arranged by size:
5117 816 638
Bme1580I G_kGCm'C
Cuts at: 1790 2406 3219 3363 3514 4632 5788 1790
Size: 616 813 144 151 1118 1156 2573
Fragments arranged by size:
2573 1156 1118 813 616 151 144
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6571
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4302 5260 442
Size: 416 53 83 399 24 2885 958 1753
Fragments arranged by size:
2885 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2487 3370 3811 2487
Size: 883 441 5247
Fragments arranged by size:
5247 883 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2139 3200 5231 1239
Size: 900 1061 2031 2579
Fragments arranged by size:
2579 2031 1061 900
Bpu10I CC'TnA_GC
Cuts at: 2234 3335 3615 3776 2234
Size: 1101 280 161 5029
Fragments arranged by size:
5029 1101 280 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3119 4696 5561 5859 3119
Size: 1577 865 298 3831
Fragments arranged by size:
3831 1577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2532 3176 5234 882
Size: 617 1033 644 2058 2219
Fragments arranged by size:
2219 2058 1033 644 617
BsaAI yAC'GTr
Cuts at: 493 1204 3405 3643 4362 493
Size: 711 2201 238 719 2702
Fragments arranged by size:
2702 2201 719 711 238
BsaBI GATnn'nnATC
Cuts at: 6565 6565
Size: 6571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2352 3054 3173 3426
Size: 53 83 186 936 819 702 119 253
Cuts at: 3426 3803 4378 4760 5395 275
Size: 377 575 382 635 1451
Fragments arranged by size:
1451 936 819 702 635 575 382 377
253 186 119 83 53
BsaWI w'CCGG_w
Cuts at: 5004 5402 5676 5823 6234 5004
Size: 398 274 147 411 5341
Fragments arranged by size:
5341 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3314 3344 1178
Size: 30 165 30 1911 30 4405
Fragments arranged by size:
4405 1911 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2818 3359 3677 1186
Size: 1632 541 318 4080
Fragments arranged by size:
4080 1632 541 318
BseYI C'CCAG_C
Cuts at: 2511 2767 3365 3724 3730 5774 6270 2511
Size: 256 598 359 6 2044 496 2812
Fragments arranged by size:
2812 2044 598 496 359 256 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 2398 3641 1163
Size: 573 381 281 1243 4093
Fragments arranged by size:
4093 1243 573 381 281
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2607 2876 3253 4021 4782 5810
Size: 275 186 606 269 377 768 761 1028
Cuts at: 5810 1540
Size: 2301
Fragments arranged by size:
2301 1028 768 761 606 377 275 269
186
BsiHKAI G_wGCw'C
Cuts at: 2240 3081 3332 3356 4632 4717 5788 2240
Size: 841 251 24 1276 85 1071 3023
Fragments arranged by size:
3023 1276 1071 841 251 85 24
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2337 2406 2473 2557 3081 3146
Size: 399 450 97 69 67 84 524 65
Cuts at: 3146 3219 3332 3356 3363 3514 3890 4632 4717
Size: 73 113 24 7 151 376 742 85
Cuts at: 4717 5788 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 524 450 399 376 151
113 97 85 84 73 69 67 65
24 7
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 3573 4460 6190 3573
Size: 887 1730 3954
Fragments arranged by size:
3954 1730 887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2376 2463 2586 3926 844
Size: 1532 87 123 1340 3489
Fragments arranged by size:
3489 1532 1340 123 87
BsrBI CCG'CTC
Cuts at: 1394 1823 3667 4458 1394
Size: 429 1844 791 3507
Fragments arranged by size:
3507 1844 791 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5066 5240 65
Size: 1183 3818 174 1396
Fragments arranged by size:
3818 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2322 5215 5402 1700
Size: 110 512 2893 187 2869
Fragments arranged by size:
2893 2869 512 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6571
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6571
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 3092 1184
Size: 1908 4663
BstXI CCAn_nnnn'nTGG
Cuts at: 2204 3010 2204
Size: 806 5765
BstYI r'GATC_y
Cuts at: 1972 3489 3690 3901 4268 4653 4670 6122 6208
Size: 1517 201 211 367 385 17 1452 86
Cuts at: 6208 6220 6566 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1517 1452 385 367 346 211 201
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3147 3231 3147
Size: 84 6487
BtgI C'CryG_G
Cuts at: 513 1065 2004 2876 3516 513
Size: 552 939 872 640 3568
Fragments arranged by size:
3568 939 872 640 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5832
Fragments arranged by size:
5832 683 56
BtsI GCAGTG_nn'
Cuts at: 3845 4087 4881 4901 3845
Size: 242 794 20 5515
Fragments arranged by size:
5515 794 242 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 3875 3875
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2595 2604
Size: 54 1425 50 275 18 330 435 9
Cuts at: 2604 2808 2873 3000 3237 3564 3867 4018 4907
Size: 204 65 127 237 327 303 151 889
Cuts at: 4907 6536 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 435 330 327 303 275
237 204 151 127 65 54 50 43
18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2604 2873 4018 1537
Size: 275 792 269 1145 4090
Fragments arranged by size:
4090 1145 792 275 269
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 3134 4501 1353
Size: 731 1050 1367 3423
Fragments arranged by size:
3423 1367 1050 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3674 5158 5532 5678 387
Size: 1064 138 2085 1484 374 146 1280
Fragments arranged by size:
2085 1484 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3079 3354 3079
Size: 275 6296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2139 2898 3200 3209 3674 4634
Size: 393 433 74 759 302 9 465 960
Cuts at: 4634 5231 6018 6286 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 960 787 759 597 465 433 393
302 268 74 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2651
Size: 6571
EcoO109I rG'GnC_Cy
Cuts at: 2037 2046 2109 2344 2402 2403 2642 2696 2752
Size: 9 63 235 58 1 239 54 56
Cuts at: 2752 2836 3387 3394 3510 3511 3635 3649 3790
Size: 84 551 7 116 1 124 14 141
Cuts at: 3790 3818 2037
Size: 28 4790
Fragments arranged by size:
4790 551 239 235 141 124 116 84
63 58 56 54 28 14 9 7
1 1
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 2590 3536 5718 1678
Size: 912 946 2182 2531
Fragments arranged by size:
2531 2182 946 912
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2728 2760 2893
Size: 32 135 32 809 32 315 32 133
Cuts at: 2893 2925 3816 3848 5212 5244 5286 5318 1373
Size: 32 891 32 1364 32 42 32 2626
Fragments arranged by size:
2626 1364 891 809 315 135 133 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 3208 3606 3915 4161 669
Size: 988 281 780 490 398 309 246 3079
Fragments arranged by size:
3079 988 780 490 398 309 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6571
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KpnI G_GTAC'C
Cuts at: 3325 3896 3325
Size: 571 6000
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5685 5869 1305
Size: 4380 184 2007
Fragments arranged by size:
4380 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 3002 3239 3566 10
Size: 54 1425 343 330 840 237 327 3015
Fragments arranged by size:
3015 1425 840 343 330 327 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3008 3572 4529 4888 5047 518
Size: 648 1842 564 957 359 159 2042
Fragments arranged by size:
2042 1842 957 648 564 359 159
MspA1I CmG'CkG
Cuts at: 1606 2305 2485 2878 3049 3421 3703 3987 4664
Size: 699 180 393 171 372 282 284 677
Cuts at: 4664 5812 6057 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 699 677 393 372 284 282
245 180 171
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5080
Fragments arranged by size:
5080 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 3784 3929 5474 1132
Size: 97 2555 145 1545 2229
Fragments arranged by size:
2555 2229 1545 145 97
PasI CC'CwG_GG
Cuts at: 1348 3317 1348
Size: 1969 4602
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3118 3397 1108
Size: 2010 279 4282
Fragments arranged by size:
4282 2010 279
PfoI T'CCnGG_A
Cuts at: 2250 2250
Size: 6571
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PmlI CAC'GTG
Cuts at: 3405 3643 3405
Size: 238 6333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2037 2046 2109 2344 2642 2696 2836 3394 3635
Size: 9 63 235 298 54 140 558 241
Cuts at: 3635 3818 2037
Size: 183 4790
Fragments arranged by size:
4790 558 298 241 235 183 140 63
54 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PspOMI G'GGCC_C
Cuts at: 2402 3510 2402
Size: 1108 5463
PspXI vC'TCGA_Gb
Cuts at: 2104 2104
Size: 6571
PstI C_TGCA'G
Cuts at: 830 2688 3923 830
Size: 1858 1235 3478
Fragments arranged by size:
3478 1858 1235
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 2305 3049 3421 2305
Size: 744 372 5455
Fragments arranged by size:
5455 744 372
SacI G_AGCT'C
Cuts at: 3081 3356 3081
Size: 275 6296
SacII CC_GC'GG
Cuts at: 2879 2879
Size: 6571
SalI G'TCGA_C
Cuts at: 1936 2716 3913 1936
Size: 780 1197 4594
Fragments arranged by size:
4594 1197 780
SanDI GG'GwC_CC
Cuts at: 2046 2642 2696 2836 3635 2046
Size: 596 54 140 799 4982
Fragments arranged by size:
4982 799 596 140 54
SapI GCTCTTCn'nnn_
Cuts at: 2084 2084
Size: 6571
SbfI CC_TGCA'GG
Cuts at: 3923 3923
Size: 6571
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SexAI A'CCwGG_T
Cuts at: 3762 3762
Size: 6571
SfcI C'TryA_G
Cuts at: 826 1045 2684 3737 3919 5054 5735 5926 826
Size: 219 1639 1053 182 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 1053 681 219 191 182
SfiI GGCCn_nnn'nGGCC
Cuts at: 2603 2999 2603
Size: 396 6175
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 2640 3898 1872
Size: 768 1258 4545
Fragments arranged by size:
4545 1258 768
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2104 3134 4675 5576 5838 820
Size: 197 926 161 1030 1541 901 262 1553
Fragments arranged by size:
1553 1541 1030 926 901 262 197 161
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3784 3929 1132
Size: 2652 145 3774
Fragments arranged by size:
3774 2652 145
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 2411 2565 1517
Size: 894 154 5523
Fragments arranged by size:
5523 894 154
StyI C'CwwG_G
Cuts at: 513 1065 2004 2203 2268 3413 3438 3814 4010
Size: 552 939 199 65 1145 25 376 196
Cuts at: 4010 513
Size: 3074
Fragments arranged by size:
3074 1145 939 552 376 199 196 65
25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2083 3020 3494 4598 4615 4768 4953
Size: 1255 503 937 474 1104 17 153 185
Cuts at: 4953 5398 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1104 937 503 474 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4817 6529 96
Size: 274 80 33 51 1443 2840 1712 138
Fragments arranged by size:
2840 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3627 3659 6531 6563 3627
Size: 32 2872 32 3635
Fragments arranged by size:
3635 2872 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2153 2722 1316
Size: 837 569 5165
Fragments arranged by size:
5165 837 569
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3760 3760
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 2104 1943
Size: 161 6410
XmaI C'CCGG_G
Cuts at: 1870 2638 3896 1870
Size: 768 1258 4545
Fragments arranged by size:
4545 1258 768
XmnI GAAnn'nnTTC
Cuts at: 4700 4700
Size: 6571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3055 3174 3427 4379
Size: 53 83 186 936 1521 119 253 952
Cuts at: 4379 276
Size: 2468
Fragments arranged by size:
2468 1521 952 936 253 186 119 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PsrI RsrII SfoI
SrfI SwaI