Restriction map for
pFN21AE4435
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaI
BseRI BsiWI BspDI BspHI BspLU11I
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI HgaI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 990 GACGTC GACGT^C
AccIII 416 TCCGGA T^CCGGA
AflIII 1126 ACACGT A^C[GA][CT]GT
AlwI 128 GGATC GGATC(4/5)
AlwNI 1186 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 41 CCTCAGG CC^T[ACGT]AGG
AspI 280 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 250 CTCGGG C^[CT]CG[GA]G
AviII 693 TGCGCA TGC^GCA
BalI 305 TGGCCA TGG^CCA
BfaI 478 CTAG C^TAG
BsaWI 416 TCCGGA [AT]^CCGG[AT]
BseAI 416 TCCGGA T^CCGGA
BsiEI 52 CGGCCG CG[GA][CT]^CG
BsmAI 1210 GAGAC GTCTC(1/5)
BsmBI 1210 GAGACG CGTCTC(1/5)
BsmI 1253 GCATTC GAATGC(1/-1)
BsoBI 250 CTCGGG C^[CT]CG[GA]G
BspEI 416 TCCGGA T^CCGGA
BspMI 11 ACCTGC ACCTGC(4/8)
BsrBI 1037 CCGCTC CCGCTC(-3/-3)
BsrDI 1268 GCAATG GCAATG(2/0)
BstEII 285 GGTCACC G^GT[ACGT]ACC
BstPI 285 GGTCACC G^GT[ACGT]ACC
Bsu36I 41 CCTCAGG CC^T[ACGT]AGG
EagI 52 CGGCCG C^GGCCG
EarI 370 GAAGAG CTCTTC(1/4)
EclXI 52 CGGCCG C^GGCCG
Eco52I 52 CGGCCG C^GGCCG
Eco81I 41 CCTCAGG CC^T[ACGT]AGG
EcoNI 957 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 285 GGTCACC G^GT[ACGT]ACC
FspI 693 TGCGCA TGC^GCA
Ksp632I 370 GAAGAG CTCTTC(1/4)
KspI 103 CCGCGG CCGC^GG
MaeI 478 CTAG C^TAG
MluNI 305 TGGCCA TGG^CCA
MroI 416 TCCGGA T^CCGGA
MscI 305 TGGCCA TGG^CCA
NcoI 522 CCATGG C^CATGG
NspI 659 GCATGT [GA]CATG^[CT]
PflFI 280 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 990 GACGTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 33 CTGCAG CTGCA^G
PvuII 699 CAGCTG CAG^CTG
SacII 103 CCGCGG CCGC^GG
SexAI 281 ACCAGGT A^CC[AT]GGT
SfcI 33 CTGCAG C^T[GA][CT]AG
SgrAI 65 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 892 CTCAAG C^T[CT][GA]AG
Tth111I 280 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 542 CCACAGACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 50 CGCG CG^CG
104 CGCG
BanI 63 GGCGCC G^G[CT][GA]CC
107 GGCGCC
BbeI 63 GGCGCC GGCGC^C
107 GGCGCC
BbrPI 220 CACGTG CAC^GTG
1127 CACGTG
BbvCI 1263 CCTCAGC CCTCAGC(-5/-2)
1030 GCTGAGG
BglII 803 AGATCT A^GATCT
845 AGATCT
BpmI 1135 CTGGAG CTGGAG(16/14)
1192 CTGGAG
BsaAI 220 CACGTG [CT]AC^GT[GA]
1127 CACGTG
BsgI 299 CTGCAC GTGCAG(16/14)
943 GTGCAG
BstUI 50 CGCG CG^CG
104 CGCG
BstXI 963 CCAGGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1235 CCAAGGACCTGG
BstYI 803 AGATCT [GA]^GATC[CT]
845 AGATCT
DraII 887 AGGTCCT [GA]G^G[ACGT]CC[CT]
1238 AGGACCT
EcoO109I 887 AGGTCCT [GA]G^G[ACGT]CC[CT]
1238 AGGACCT
FseI 53 GGCCGGCC GGCCGG^CC
1081 GGCCGGCC
HaeII 63 GGCGCC [GA]GCGC^[CT]
107 GGCGCC
HinfI 295 GACTC G^A[ACGT]TC
676 GAGTC
KasI 63 GGCGCC G^GCGCC
107 GGCGCC
MflI 803 AGATCT [GA]^GATC[CT]
845 AGATCT
MslI 517 CACGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
654 CATGGGCATG
MvnI 50 CGCG CG^CG
104 CGCG
NarI 63 GGCGCC GG^CGCC
107 GGCGCC
PflMI 592 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1185 CCAGATGCTGG
PleI 295 GACTC GAGTC(4/5)
676 GAGTC
PmaCI 220 CACGTG CAC^GTG
1127 CACGTG
PmlI 220 CACGTG CAC^GTG
1127 CACGTG
PpuMI 887 AGGTCCT [GA]G^G[AT]CC[CT]
1238 AGGACCT
SfaNI 1188 GATGC GCATC(5/9)
1112 GCATC
SfoI 63 GGCGCC GGC^GCC
107 GGCGCC
TaqI 833 TCGA T^CGA
1313 TCGA
TthHB8I 833 TCGA T^CGA
1313 TCGA
Van91I 592 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1185 CCAGATGCTGG
XhoII 803 AGATCT [GA]^GATC[CT]
845 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaII BanII BbsI BbvI BcnI
BmyI BpuAI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I Bsp1407I
BsrFI BsrGI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SspBI StyI TseI Tsp45I
TspRI