(Linear) (Six-base) MAPSORT of: KIEE4435.seq Check: 6299 from: 1 to: 1356 KIEE4435.orf With 182 enzymes: SgfI * March 22, 2012 18:01 .. AatII G_ACGT'C Cuts at: 0 994 1356 Size: 994 362 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 1356 Size: 403 953 AflIII A'CryG_T Cuts at: 0 1126 1356 Size: 1126 230 AlwNI CAG_nnn'CTG Cuts at: 0 1191 1356 Size: 1191 165 AvaI C'yCGr_G Cuts at: 0 250 1356 Size: 250 1106 BanI G'GyrC_C Cuts at: 0 63 107 1356 Size: 63 44 1249 Fragments arranged by size: 1249 63 44 BanII G_rGCy'C Cuts at: 0 257 452 1344 1356 Size: 257 195 892 12 Fragments arranged by size: 892 257 195 12 BbeI G_GCGC'C Cuts at: 0 67 111 1356 Size: 67 44 1245 Fragments arranged by size: 1245 67 44 BbsI GAAGACnn'nnnn_ Cuts at: 0 432 576 890 1024 1356 Size: 432 144 314 134 332 Fragments arranged by size: 432 332 314 144 134 BbvCI CC'TCA_GC Cuts at: 0 1031 1264 1356 Size: 1031 233 92 Fragments arranged by size: 1031 233 92 BglII A'GATC_T Cuts at: 0 803 845 1356 Size: 803 42 511 Fragments arranged by size: 803 511 42 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 699 1356 Size: 699 657 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1156 1213 1356 Size: 1156 57 143 Fragments arranged by size: 1156 143 57 Bpu10I CC'TnA_GC Cuts at: 0 884 1031 1264 1356 Size: 884 147 233 92 Fragments arranged by size: 884 233 147 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 877 1356 Size: 877 479 BsaAI yAC'GTr Cuts at: 0 222 1129 1356 Size: 222 907 227 Fragments arranged by size: 907 227 222 BsaHI Gr'CG_yC Cuts at: 0 64 108 991 1356 Size: 64 44 883 365 Fragments arranged by size: 883 365 64 44 BsaWI w'CCGG_w Cuts at: 0 416 1356 Size: 416 940 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1186 1216 1356 Size: 1186 30 140 Fragments arranged by size: 1186 140 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 284 964 1356 Size: 284 680 392 Fragments arranged by size: 680 392 284 BsiEI CG_ry'CG Cuts at: 0 55 1356 Size: 55 1301 BsiHKAI G_wGCw'C Cuts at: 0 748 860 1073 1142 1289 1356 Size: 748 112 213 69 147 67 Fragments arranged by size: 748 213 147 112 69 67 BsmI GAATG_Cn' Cuts at: 0 1253 1356 Size: 1253 103 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1204 1356 Size: 1204 152 Bsp1286I G_dGCh'C Cuts at: 0 257 452 748 860 1073 1142 1289 1344 Size: 257 195 296 112 213 69 147 55 Cuts at: 1344 1356 Size: 12 Fragments arranged by size: 296 257 213 195 147 112 69 55 12 BspEI T'CCGG_A Cuts at: 0 416 1356 Size: 416 940 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 20 1356 Size: 20 1336 BsrBI CCG'CTC Cuts at: 0 1039 1356 Size: 1039 317 BsrDI GCAATG_nn' Cuts at: 0 1275 1356 Size: 1275 81 BsrFI r'CCGG_y Cuts at: 0 4 54 66 565 860 1082 1356 Size: 4 50 12 499 295 222 274 Fragments arranged by size: 499 295 274 222 50 12 4 BsrGI T'GTAC_A Cuts at: 0 626 662 737 1356 Size: 626 36 75 619 Fragments arranged by size: 626 619 75 36 BstEII G'GTnAC_C Cuts at: 0 285 1356 Size: 285 1071 BstXI CCAn_nnnn'nTGG Cuts at: 0 970 1242 1356 Size: 970 272 114 Fragments arranged by size: 970 272 114 BstYI r'GATC_y Cuts at: 0 803 845 1356 Size: 803 42 511 Fragments arranged by size: 803 511 42 Bsu36I CC'TnA_GG Cuts at: 0 42 1356 Size: 42 1314 BtgI C'CryG_G Cuts at: 0 103 146 522 1301 1356 Size: 103 43 376 779 55 Fragments arranged by size: 779 376 103 55 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1089 1356 Size: 1089 267 BtsI GCAGTG_nn' Cuts at: 0 609 1356 Size: 609 747 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 531 566 1356 Size: 531 35 790 Fragments arranged by size: 790 531 35 EaeI y'GGCC_r Cuts at: 0 2 52 305 730 1356 Size: 2 50 253 425 626 Fragments arranged by size: 626 425 253 50 2 EagI C'GGCC_G Cuts at: 0 52 1356 Size: 52 1304 EarI CTCTTCn'nnn_ Cuts at: 0 365 1356 Size: 365 991 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 1156 1213 1356 Size: 403 753 57 143 Fragments arranged by size: 753 403 143 57 EcoNI CCTnn'n_nnAGG Cuts at: 0 961 1356 Size: 961 395 EcoO109I rG'GnC_Cy Cuts at: 0 888 1239 1356 Size: 888 351 117 Fragments arranged by size: 888 351 117 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 810 846 1356 Size: 810 36 510 Fragments arranged by size: 810 510 36 FseI GG_CCGG'CC Cuts at: 0 58 1086 1356 Size: 58 1028 270 Fragments arranged by size: 1028 270 58 FspI TGC'GCA Cuts at: 0 695 1356 Size: 695 661 HaeII r_GCGC'y Cuts at: 0 67 111 1356 Size: 67 44 1245 Fragments arranged by size: 1245 67 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 931 963 1356 Size: 931 32 393 Fragments arranged by size: 931 393 32 KasI G'GCGC_C Cuts at: 0 63 107 1356 Size: 63 44 1249 Fragments arranged by size: 1249 63 44 MscI TGG'CCA Cuts at: 0 307 1356 Size: 307 1049 MslI CAynn'nnrTG Cuts at: 0 521 658 1356 Size: 521 137 698 Fragments arranged by size: 698 521 137 MspA1I CmG'CkG Cuts at: 0 105 701 735 1356 Size: 105 596 34 621 Fragments arranged by size: 621 596 105 34 NaeI GCC'GGC Cuts at: 0 6 56 68 567 1084 1356 Size: 6 50 12 499 517 272 Fragments arranged by size: 517 499 272 50 12 6 NarI GG'CG_CC Cuts at: 0 64 108 1356 Size: 64 44 1248 Fragments arranged by size: 1248 64 44 NcoI C'CATG_G Cuts at: 0 522 1356 Size: 522 834 NgoMIV G'CCGG_C Cuts at: 0 4 54 66 565 1082 1356 Size: 4 50 12 499 517 274 Fragments arranged by size: 517 499 274 50 12 4 NspI r_CATG'y Cuts at: 0 663 1356 Size: 663 693 PasI CC'CwG_GG Cuts at: 0 963 1344 1356 Size: 963 381 12 Fragments arranged by size: 963 381 12 PflMI CCAn_nnn'nTGG Cuts at: 0 598 1191 1356 Size: 598 593 165 Fragments arranged by size: 598 593 165 PfoI T'CCnGG_A Cuts at: 0 443 797 1356 Size: 443 354 559 Fragments arranged by size: 559 443 354 PmlI CAC'GTG Cuts at: 0 222 1129 1356 Size: 222 907 227 Fragments arranged by size: 907 227 222 PpuMI rG'GwC_Cy Cuts at: 0 888 1239 1356 Size: 888 351 117 Fragments arranged by size: 888 351 117 PshAI GACnn'nnGTC Cuts at: 0 994 1356 Size: 994 362 PstI C_TGCA'G Cuts at: 0 37 1356 Size: 37 1319 PvuII CAG'CTG Cuts at: 0 701 1356 Size: 701 655 SacII CC_GC'GG Cuts at: 0 106 1356 Size: 106 1250 SexAI A'CCwGG_T Cuts at: 0 281 1356 Size: 281 1075 SfcI C'TryA_G Cuts at: 0 33 1356 Size: 33 1323 SfoI GGC'GCC Cuts at: 0 65 109 1356 Size: 65 44 1247 Fragments arranged by size: 1247 65 44 SgrAI Cr'CCGG_yG Cuts at: 0 66 1356 Size: 66 1290 SmlI C'TyrA_G Cuts at: 0 892 1356 Size: 892 464 StyI C'CwwG_G Cuts at: 0 522 978 1235 1356 Size: 522 456 257 121 Fragments arranged by size: 522 456 257 121 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 325 841 1356 Size: 325 516 515 Fragments arranged by size: 516 515 325 TatI w'GTAC_w Cuts at: 0 317 533 626 662 737 1356 Size: 317 216 93 36 75 619 Fragments arranged by size: 619 317 216 93 75 36 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 464 496 1186 1218 1356 Size: 464 32 690 32 138 Fragments arranged by size: 690 464 138 32 32 Tth111I GACn'n_nGTC Cuts at: 0 283 1356 Size: 283 1073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 549 1356 Size: 549 807 ZraI GAC'GTC Cuts at: 0 992 1356 Size: 992 364 Enzymes that do cut: AatII AcuI AflIII AlwNI AvaI BanI BanII BbeI BbsI BbvCI BglII BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI PstI PvuII SacII SexAI SfcI SfoI SgrAI SmlI StyI TaqII TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BsaI BsaBI BseRI BseYI BsiWI BspHI BssHII BssSI BstAPI BstBI BstZ17I ClaI DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI