Restriction map for
pFN21AE3629
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MamI
MluI MluNI MscI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI Sse8387I SspBI SspI SwaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 2183 TCCGGA T^CCGGA
AciI 1785 GCGG CCGC(-3/-1)
AflIII 1548 ACATGT A^C[GA][CT]GT
ApaI 2277 GGGCCC GGGCC^C
Asp700I 817 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1974 GGCACC G^G[CT][GA]CC
BclI 297 TGATCA T^GATCA
BsaI 350 GGTCTC GGTCTC(1/5)
BsaWI 2183 TCCGGA [AT]^CCGG[AT]
BseAI 2183 TCCGGA T^CCGGA
BsgI 843 CTGCAC GTGCAG(16/14)
BsmFI 1787 GGGAC GGGAC(10/14)
BspEI 2183 TCCGGA T^CCGGA
BspHI 1596 TCATGA T^CATGA
BspLU11I 1548 ACATGT A^CATGT
BspMI 642 ACCTGC ACCTGC(4/8)
BsrFI 1790 ACCGGC [GA]^CCGG[CT]
CfoI 96 GCGC GCG^C
Cfr10I 1790 ACCGGC [GA]^CCGG[CT]
DrdI 2013 GACAGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2068 CCACGG C^C[GA][CT]GG
EcoRI 1737 GAATTC G^AATTC
EcoRV 697 GATATC GAT^ATC
EcoT22I 1487 ATGCAT ATGCA^T
FauI 1785 GCGGG CCCGC(4/6)
FbaI 297 TGATCA T^GATCA
HaeII 95 GGCGCT [GA]GCGC^[CT]
HhaI 96 GCGC GCG^C
HinP1I 96 GCGC G^CGC
HindIII 879 AAGCTT A^AGCTT
MaeII 201 ACGT A^CGT
MfeI 1262 CAATTG C^AATTG
MroI 2183 TCCGGA T^CCGGA
MunI 1262 CAATTG C^AATTG
NsiI 1487 ATGCAT ATGCA^T
PpuMI 2084 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1596 TCATGA T^CATGA
SacI 2104 GAGCTC GAGCT^C
SpeI 174 ACTAGT A^CTAGT
SphI 1923 GCATGC GCATG^C
StuI 2436 AGGCCT AGG^CCT
XbaI 2403 TCTAGA T^CTAGA
XmnI 817 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
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AhdI 173 GACTAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2047 GACCTGAAGTC
AlwNI 129 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1777 CAGCAGCTG
AocI 262 CCTGAGG CC^T[ACGT]AGG
691 CCTGAGG
AseI 585 ATTAAT AT^TAAT
2416 ATTAAT
AsnI 585 ATTAAT AT^TAAT
2416 ATTAAT
AspEI 173 GACTAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2047 GACCTGAAGTC
AspHI 1442 GTGCTC G[AT]GC[AT]^C
2104 GAGCTC
AspI 887 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
930 GACATTGTC
AvaII 1788 GGACC G^G[AT]CC
2085 GGACC
BanII 2104 GAGCTC G[GA]GC[CT]^C
2277 GGGCCC
BpmI 2296 CTCCAG CTGGAG(16/14)
782 CTGGAG
BsiHKAI 1442 GTGCTC G[AT]GC[AT]^C
2104 GAGCTC
BsmAI 351 GTCTC GTCTC(1/5)
1398 GTCTC
BsrDI 320 CATTGC GCAATG(2/0)
53 GCAATG
Bsu36I 262 CCTGAGG CC^T[ACGT]AGG
691 CCTGAGG
DraIII 1436 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2368 CACCTTGTG
Eam1105I 173 GACTAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2047 GACCTGAAGTC
Eco81I 262 CCTGAGG CC^T[ACGT]AGG
691 CCTGAGG
EcoT14I 1478 CCTTGG C^C[AT][AT]GG
1660 CCTTGG
HapII 1791 CCGG C^CGG
2184 CCGG
HpaII 1791 CCGG C^CGG
2184 CCGG
MslI 1618 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2286 CACTGCTATG
MspI 1791 CCGG C^CGG
2184 CCGG
PflFI 887 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
930 GACATTGTC
PflMI 138 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1655 CCATCCCTTGG
PshBI 585 ATTAAT AT^TAAT
2416 ATTAAT
PstI 1049 CTGCAG CTGCA^G
2255 CTGCAG
SapI 478 GAAGAGC GCTCTTC(1/4)
1219 GCTCTTC
SfaNI 1258 GATGC GCATC(5/9)
2225 GCATC
StyI 1478 CCTTGG C^C[AT][AT]GG
1660 CCTTGG
Tth111I 887 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
930 GACATTGTC
Van91I 138 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1655 CCATCCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BciVI BfaI BglII
BmyI BpuAI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII