(Circular) (Six-base) MAPSORT of: pFN21AE3629.seq Check: 1564 from: 1 to: 7171 pFN21AE3629 7171 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4981 278 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4514 1937 Size: 2577 4594 Acc65I G'GTAC_C Cuts at: 4492 4492 Size: 7171 AclI AA'CG_TT Cuts at: 1563 5298 5671 1563 Size: 3735 373 3063 Fragments arranged by size: 3735 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 3764 4076 5234 6618 6886 1632 Size: 870 1262 312 1158 1384 268 1917 Fragments arranged by size: 1917 1384 1262 1158 870 312 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6974 AflIII A'CryG_T Cuts at: 1203 3553 6070 7047 1203 Size: 2350 2517 977 1327 Fragments arranged by size: 2517 2350 1327 977 AgeI A'CCGG_T Cuts at: 6002 6002 Size: 7171 AhdI GACnn_n'nnGTC Cuts at: 2183 4057 5900 2183 Size: 1874 1843 3454 Fragments arranged by size: 3454 1874 1843 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7171 AlwNI CAG_nnn'CTG Cuts at: 2139 3787 4914 6486 2139 Size: 1648 1127 1572 2824 Fragments arranged by size: 2824 1648 1572 1127 ApaI G_GGCC'C Cuts at: 4286 4286 Size: 7171 ApaLI G'TGCA_C Cuts at: 5228 6384 5228 Size: 1156 6015 ApoI r'AATT_y Cuts at: 1493 2080 2091 2286 2432 3371 3708 3742 4480 Size: 587 11 195 146 939 337 34 738 Cuts at: 4480 4706 4858 6967 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 939 738 587 337 226 195 152 146 34 11 AseI AT'TA_AT Cuts at: 160 1032 2591 4422 5725 160 Size: 872 1559 1831 1303 1606 Fragments arranged by size: 1831 1606 1559 1303 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 AvaI C'yCGr_G Cuts at: 1870 1943 4496 1870 Size: 73 2553 4545 Fragments arranged by size: 4545 2553 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2916 2949 3551 3584 2916 Size: 33 602 33 6503 Fragments arranged by size: 6503 602 33 33 BamHI G'GATC_C Cuts at: 4501 4868 4501 Size: 367 6804 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3979 4492 5947 618 Size: 325 217 627 2192 513 1455 1842 Fragments arranged by size: 2192 1842 1455 627 513 325 217 BanII G_rGCy'C Cuts at: 1391 4113 4286 4490 1391 Size: 2722 173 204 4072 Fragments arranged by size: 4072 2722 204 173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2223 3571 4206 928 Size: 971 324 1348 635 3893 Fragments arranged by size: 3893 1348 971 635 324 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5362 5396 1903 Size: 34 3425 34 3678 Fragments arranged by size: 3678 3425 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2259 3085 3718 5063 6273 2259 Size: 826 633 1345 1210 3157 Fragments arranged by size: 3157 1345 1210 826 633 BclI T'GATC_A Cuts at: 1548 2302 1548 Size: 754 6417 BfrBI ATG'CAT Cuts at: 3494 3494 Size: 7171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5782 136 Size: 107 122 71 631 605 4110 1525 Fragments arranged by size: 4110 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2533 2750 3649 4021 7166 2533 Size: 217 899 372 3145 2538 Fragments arranged by size: 3145 2538 899 372 217 BlpI GC'TnA_GC Cuts at: 4588 4588 Size: 7171 Bme1580I G_kGCm'C Cuts at: 1790 4286 5232 6388 1790 Size: 2496 946 1156 2573 Fragments arranged by size: 2573 2496 1156 946 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7171 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4902 5860 442 Size: 416 53 83 399 24 3485 958 1753 Fragments arranged by size: 3485 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2808 4286 5831 1239 Size: 1569 1478 1545 2579 Fragments arranged by size: 2579 1569 1545 1478 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5296 6161 6459 5296 Size: 865 298 6008 Fragments arranged by size: 6008 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2361 5834 882 Size: 617 862 3473 2219 Fragments arranged by size: 3473 2219 862 617 BsaAI yAC'GTr Cuts at: 493 1204 4962 493 Size: 711 3758 2702 Fragments arranged by size: 3758 2702 711 BsaBI GATnn'nnATC Cuts at: 7165 7165 Size: 7171 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4978 5360 5995 275 Size: 53 83 186 936 3445 382 635 1451 Fragments arranged by size: 3445 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 5604 6002 6276 6423 6834 4188 Size: 1416 398 274 147 411 4525 Fragments arranged by size: 4525 1416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4155 4185 1178 Size: 30 165 30 2752 30 4164 Fragments arranged by size: 4164 2752 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3186 4436 4452 1186 Size: 2000 1250 16 3905 Fragments arranged by size: 3905 2000 1250 16 BseYI C'CCAG_C Cuts at: 4139 6374 6870 4139 Size: 2235 496 4440 Fragments arranged by size: 4440 2235 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2833 1163 Size: 573 1097 5501 Fragments arranged by size: 5501 1097 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4621 5382 6410 1540 Size: 275 186 2620 761 1028 2301 Fragments arranged by size: 2620 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3451 4113 5232 5317 6388 3451 Size: 662 1119 85 1071 4234 Fragments arranged by size: 4234 1119 1071 662 85 BsmI GAATG_Cn' Cuts at: 4682 4775 4682 Size: 93 7078 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3451 4113 4286 4490 5232 5317 6388 Size: 399 1661 662 173 204 742 85 1071 Cuts at: 6388 1391 Size: 2174 Fragments arranged by size: 2174 1661 1071 742 662 399 204 173 85 BspEI T'CCGG_A Cuts at: 4188 6834 4188 Size: 2646 4525 BspHI T'CATG_A Cuts at: 3601 5060 6790 3601 Size: 1459 1730 3982 Fragments arranged by size: 3982 1730 1459 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2656 4526 844 Size: 1812 1870 3489 Fragments arranged by size: 3489 1870 1812 BsrBI CCG'CTC Cuts at: 1394 1823 5058 1394 Size: 429 3235 3507 Fragments arranged by size: 3507 3235 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2065 2324 5666 5840 65 Size: 1183 817 259 3342 174 1396 Fragments arranged by size: 3342 1396 1183 817 259 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3795 5815 6002 1700 Size: 110 1985 2020 187 2869 Fragments arranged by size: 2869 2020 1985 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7171 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7171 BssSI C'ACGA_G Cuts at: 5231 6243 5231 Size: 1012 6159 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7171 BstBI TT'CG_AA Cuts at: 5975 5975 Size: 7171 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7171 BstYI r'GATC_y Cuts at: 1972 2533 2750 3649 4006 4021 4501 4868 5253 Size: 561 217 899 357 15 480 367 385 Cuts at: 5253 5270 6722 6808 6820 7166 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 899 561 480 385 367 357 346 217 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2268 2697 2268 Size: 429 6742 BtgI C'CryG_G Cuts at: 513 1065 2004 4073 513 Size: 552 939 2069 3611 Fragments arranged by size: 3611 2069 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6432 Fragments arranged by size: 6432 683 56 BtsI GCAGTG_nn' Cuts at: 3967 4257 4290 4687 5481 5501 3967 Size: 290 33 397 794 20 5637 Fragments arranged by size: 5637 794 397 290 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7171 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7136 DraI TTT'AAA Cuts at: 4475 4475 Size: 7171 DraIII CAC_nnn'GTG Cuts at: 3446 4378 3446 Size: 932 6239 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4024 6178 809 Size: 507 2708 2154 1802 Fragments arranged by size: 2708 2154 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4618 5507 7136 Size: 54 1425 50 275 18 2788 889 1629 Cuts at: 7136 8 Size: 43 Fragments arranged by size: 2788 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4618 1537 Size: 275 2806 4090 Fragments arranged by size: 4090 2806 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 3128 3231 3839 5101 1353 Size: 1125 650 103 608 1262 3423 Fragments arranged by size: 3423 1262 1125 650 608 103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5758 6132 6278 387 Size: 1064 138 4169 374 146 1280 Fragments arranged by size: 4169 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4111 4111 Size: 7171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2502 2808 3764 4076 4286 5234 5831 Size: 393 870 306 956 312 210 948 597 Cuts at: 5831 6618 6886 1239 Size: 787 268 1524 Fragments arranged by size: 1524 956 948 870 787 597 393 312 306 268 210 EcoO109I rG'GnC_Cy Cuts at: 4090 4282 4283 4090 Size: 192 1 6978 Fragments arranged by size: 6978 192 1 EcoRI G'AATT_C Cuts at: 3742 4480 4858 3742 Size: 738 378 6055 Fragments arranged by size: 6055 738 378 EcoRV GAT'ATC Cuts at: 2704 2704 Size: 7171 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2240 3933 4289 2240 Size: 1693 356 5122 Fragments arranged by size: 5122 1693 356 FspI TGC'GCA Cuts at: 5677 5677 Size: 7171 HaeII r_GCGC'y Cuts at: 1678 2104 6318 1678 Size: 426 4214 2531 Fragments arranged by size: 4214 2531 426 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2055 2087 2169 2201 2339 Size: 32 135 32 483 32 82 32 138 Cuts at: 2339 2371 2894 2926 2999 3031 3607 3639 3867 Size: 32 523 32 73 32 576 32 228 Cuts at: 3867 3899 4023 4043 4055 4075 4375 4407 5812 Size: 32 124 20 12 20 300 32 1405 Cuts at: 5812 5844 5886 5918 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1405 576 523 483 300 228 138 135 124 82 73 42 32 32 32 32 32 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2815 3187 4183 4515 4761 669 Size: 988 281 877 372 996 332 246 3079 Fragments arranged by size: 3079 996 988 877 372 332 281 246 HindIII A'AGCT_T Cuts at: 748 2884 748 Size: 2136 5035 HpaI GTT'AAC Cuts at: 4761 4761 Size: 7171 KpnI G_GTAC'C Cuts at: 4496 4496 Size: 7171 MfeI C'AATT_G Cuts at: 3267 4770 3267 Size: 1503 5668 MluI A'CGCG_T Cuts at: 7047 7047 Size: 7171 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3878 6285 6469 1305 Size: 2573 2407 184 2007 Fragments arranged by size: 2573 2407 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5349 Fragments arranged by size: 5349 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3627 4295 5129 5488 5647 518 Size: 648 2461 668 834 359 159 2042 Fragments arranged by size: 2461 2042 834 668 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3059 3787 4358 4587 5264 6412 6657 1606 Size: 1453 728 571 229 677 1148 245 2120 Fragments arranged by size: 2120 1453 1148 728 677 571 245 229 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5680 Fragments arranged by size: 5680 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7171 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7171 NotI GC'GGCC_GC Cuts at: 4618 4618 Size: 7171 NsiI A_TGCA'T Cuts at: 3496 3496 Size: 7171 NspI r_CATG'y Cuts at: 1132 1229 2311 3498 3557 3932 4343 4529 6074 Size: 97 1082 1187 59 375 411 186 1545 Cuts at: 6074 1132 Size: 2229 Fragments arranged by size: 2229 1545 1187 1082 411 375 186 97 59 PasI CC'CwG_GG Cuts at: 1348 4279 1348 Size: 2931 4240 PciI A'CATG_T Cuts at: 3553 6070 3553 Size: 2517 4654 PflMI CCAn_nnn'nTGG Cuts at: 1108 2149 3666 1108 Size: 1041 1517 4613 Fragments arranged by size: 4613 1517 1041 PmeI GTTT'AAAC Cuts at: 4475 4475 Size: 7171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5240 5272 6753 6785 5240 Size: 32 1481 32 5626 Fragments arranged by size: 5626 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4090 4090 Size: 7171 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7171 PsiI TTA'TAA Cuts at: 2507 4741 2507 Size: 2234 4937 PspOMI G'GGCC_C Cuts at: 4282 4282 Size: 7171 PstI C_TGCA'G Cuts at: 830 3058 4264 4523 830 Size: 2228 1206 259 3478 Fragments arranged by size: 3478 2228 1206 259 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7171 PvuII CAG'CTG Cuts at: 3059 3787 4358 3059 Size: 728 571 5872 Fragments arranged by size: 5872 728 571 SacI G_AGCT'C Cuts at: 4113 4113 Size: 7171 SalI G'TCGA_C Cuts at: 1936 4513 1936 Size: 2577 4594 SapI GCTCTTCn'nnn_ Cuts at: 2478 3231 2478 Size: 753 6418 SbfI CC_TGCA'GG Cuts at: 4523 4523 Size: 7171 ScaI AGT'ACT Cuts at: 5419 7131 5419 Size: 1712 5459 SfcI C'TryA_G Cuts at: 826 1045 3054 3291 4260 4519 5654 6335 6526 Size: 219 2009 237 969 259 1135 681 191 Cuts at: 6526 826 Size: 1471 Fragments arranged by size: 2009 1471 1135 969 681 259 237 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7171 SmaI CCC'GGG Cuts at: 1872 4498 1872 Size: 2626 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5275 6176 6438 820 Size: 197 926 3332 901 262 1553 Fragments arranged by size: 3332 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4962 493 Size: 4469 2702 SpeI A'CTAG_T Cuts at: 152 2179 152 Size: 2027 5144 SphI G_CATG'C Cuts at: 1132 3932 4529 1132 Size: 2800 597 3774 Fragments arranged by size: 3774 2800 597 SspI AAT'ATT Cuts at: 5 52 5095 5 Size: 47 5043 2081 Fragments arranged by size: 5043 2081 47 StuI AGG'CCT Cuts at: 1517 4443 1517 Size: 2926 4245 StyI C'CwwG_G Cuts at: 513 1065 2004 3483 3665 4610 513 Size: 552 939 1479 182 945 3074 Fragments arranged by size: 3074 1479 945 939 552 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2141 4153 5198 5215 5368 5553 5998 Size: 1255 561 2012 1045 17 153 185 445 Cuts at: 5998 325 Size: 1498 Fragments arranged by size: 2012 1498 1255 1045 561 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5417 7129 96 Size: 274 80 33 51 1443 3440 1712 138 Fragments arranged by size: 3440 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3271 3303 7131 7163 3271 Size: 32 3828 32 3279 Fragments arranged by size: 3828 3279 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2895 2938 1316 Size: 1579 43 5549 Fragments arranged by size: 5549 1579 43 XbaI T'CTAG_A Cuts at: 4408 4507 4408 Size: 99 7072 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7171 XmaI C'CCGG_G Cuts at: 1870 4496 1870 Size: 2626 4545 XmnI GAAnn'nnTTC Cuts at: 2826 5300 2826 Size: 2474 4697 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4979 276 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI