################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AccIII AclI AcsI AflII AflIII AgeI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI DsaI EagI EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HinfI HpaI KasI KspI MaeIII MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tsp45I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 460 GACGTC GACGT^C Acc65I 572 GGTACC G^GTACC AccII 269 CGCG CG^CG AcyI 460 GACGTC G[GA]^CG[CT]C AocI 993 CCTGAGG CC^T[ACGT]AGG Aor51HI 824 AGCGCT AGC^GCT ApaI 341 GGGCCC GGGCC^C Asp718I 572 GGTACC G^GTACC AspHI 1372 GAGCTC G[AT]GC[AT]^C AspI 720 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 338 CCCGGG C^[CT]CG[GA]G AviII 802 TGCGCA TGC^GCA BcgI 242 GCAGCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1126 CTAG C^TAG BlpI 1173 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1173 GCTCAGC GC^T[ACGT]AGC BsaHI 460 GACGTC G[GA]^CG[CT]C BseRI 300 CTCCTC GAGGAG(10/8) BsiEI 954 CGACCG CG[GA][CT]^CG BsiHKAI 1372 GAGCTC G[AT]GC[AT]^C BsoBI 338 CCCGGG C^[CT]CG[GA]G Bsp1407I 1214 TGTACA T^GTACA BspHI 593 TCATGA T^CATGA BsrDI 470 GCAATG GCAATG(2/0) BsrFI 182 ACCGGC [GA]^CCGG[CT] BsrGI 1214 TGTACA T^GTACA BstAPI 239 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 269 CGCG CG^CG BstYI 755 AGATCC [GA]^GATC[CT] Bsu36I 993 CCTGAGG CC^T[ACGT]AGG CelII 1173 GCTCAGC GC^T[ACGT]AGC Cfr10I 182 ACCGGC [GA]^CCGG[CT] DraIII 603 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 824 AGCGCT AGC^GCT Eco81I 993 CCTGAGG CC^T[ACGT]AGG FspI 802 TGCGCA TGC^GCA HgaI 778 GACGC GACGC(5/10) Hin1I 460 GACGTC G[GA]^CG[CT]C HincII 145 GTTGAC GT[CT]^[GA]AC HindII 145 GTTGAC GT[CT]^[GA]AC KpnI 572 GGTACC GGTAC^C MaeI 1126 CTAG C^TAG MflI 755 AGATCC [GA]^GATC[CT] MseI 1067 TTAA T^TAA MvnI 269 CGCG CG^CG PflFI 720 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 417 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 243 CAGCTG CAG^CTG RcaI 593 TCATGA T^CATGA SacI 1372 GAGCTC GAGCT^C SapI 1044 GCTCTTC GCTCTTC(1/4) SmaI 338 CCCGGG CCC^GGG SspBI 1214 TGTACA T^GTACA StuI 921 AGGCCT AGG^CCT Tru9I 1067 TTAA T^TAA Tsp509I 19 AATT ^AATT Tth111I 720 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 417 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 755 AGATCC [GA]^GATC[CT] XmaCI 338 CCCGGG C^CCGGG XmaI 338 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 79 GTCTAC GT^[AC][GT]AC 1285 GTAGAC AhdI 148 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 584 GACACAATGTC AspEI 148 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 584 GACACAATGTC BalI 131 TGGCCA TGG^CCA 425 TGGCCA BanI 572 GGTACC G^G[CT][GA]CC 876 GGTGCC BsaI 534 GAGACC GGTCTC(1/5) 1023 GAGACC BsiYI 42 CCCTCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 417 CCACTGCCTGG BslI 42 CCCTCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 417 CCACTGCCTGG BsmBI 458 GAGACG CGTCTC(1/5) 505 GAGACG BstXI 38 CCACCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1319 CCAACTTCCTGG Cac8I 427 GCCAGC GC[ACGT]^[ACGT]GC 899 GCTGGC EaeI 131 TGGCCA [CT]^GGCC[GA] 425 TGGCCA Eam1105I 148 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 584 GACACAATGTC EarI 1045 CTCTTC CTCTTC(1/4) 70 GAAGAG Ksp632I 1045 CTCTTC CTCTTC(1/4) 70 GAAGAG MboII 70 GAAGA GAAGA(8/7) 1046 TCTTC MluNI 131 TGGCCA TGG^CCA 425 TGGCCA MscI 131 TGGCCA TGG^CCA 425 TGGCCA MslI 309 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 679 CACTTATATG PpuMI 60 AGGACCC [GA]G^G[AT]CC[CT] 989 GGGACCT PstI 237 CTGCAG CTGCA^G 1273 CTGCAG SfcI 237 CTGCAG C^T[GA][CT]AG 1273 CTGCAG TaqI 512 TCGA T^CGA 582 TCGA TthHB8I 512 TCGA T^CGA 582 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanII BbvI BclI BcnI BmyI BpmI BsaJI BsgI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI XcmI