(Circular) (Six-base) MAPSORT of: pFN21AE1307.seq Check: 7932 from: 1 to: 6121 pFN21AE1307 6121 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2249 2249 Size: 6121 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2469 3931 278 Size: 53 83 186 936 933 1462 2468 Fragments arranged by size: 2468 1462 936 933 186 83 53 AccI GT'mk_AC Cuts at: 1937 2085 3291 3464 1937 Size: 148 1206 173 4594 Fragments arranged by size: 4594 1206 173 148 Acc65I G'GTAC_C Cuts at: 2577 3442 2577 Size: 865 5256 AclI AA'CG_TT Cuts at: 1563 4248 4621 1563 Size: 2685 373 3063 Fragments arranged by size: 3063 2685 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 2959 3332 4184 5568 5836 1632 Size: 596 731 373 852 1384 268 1917 Fragments arranged by size: 1917 1384 852 731 596 373 268 AfeI AGC'GCT Cuts at: 2831 2831 Size: 6121 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 5020 5997 1203 Size: 3817 977 1327 Fragments arranged by size: 3817 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 2158 2594 4850 2158 Size: 436 2256 3429 Fragments arranged by size: 3429 2256 436 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3002 1184 Size: 1818 4303 AlwNI CAG_nnn'CTG Cuts at: 2117 2250 2424 2728 2786 2982 3228 3864 5436 Size: 133 174 304 58 196 246 636 1572 Cuts at: 5436 2117 Size: 2802 Fragments arranged by size: 2802 1572 636 304 246 196 174 133 58 ApaI G_GGCC'C Cuts at: 2350 2350 Size: 6121 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 3430 3656 3808 5917 1493 Size: 1937 226 152 2109 1697 Fragments arranged by size: 2109 1937 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 2343 3446 1870 Size: 73 400 1103 4545 Fragments arranged by size: 4545 1103 400 73 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2577 2881 3442 4897 618 Size: 325 217 627 790 304 561 1455 1842 Fragments arranged by size: 1842 1455 790 627 561 325 304 217 BanII G_rGCy'C Cuts at: 1391 2336 2343 2350 2376 3129 3354 3381 3440 Size: 945 7 7 26 753 225 27 59 Cuts at: 3440 1391 Size: 4072 Fragments arranged by size: 4072 945 753 225 59 27 26 7 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2236 2270 4312 4346 1903 Size: 34 299 34 2042 34 3678 Fragments arranged by size: 3678 2042 299 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 5223 4013 Size: 1210 4911 BclI T'GATC_A Cuts at: 1548 2448 2601 2931 1548 Size: 900 153 330 4738 Fragments arranged by size: 4738 900 330 153 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4732 136 Size: 107 122 71 631 605 3060 1525 Fragments arranged by size: 3060 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6116 6116 Size: 6121 BlpI GC'TnA_GC Cuts at: 3179 3538 3179 Size: 359 5762 Bme1580I G_kGCm'C Cuts at: 1790 2350 2886 4182 5338 1790 Size: 560 536 1296 1156 2573 Fragments arranged by size: 2573 1296 1156 560 536 BmgBI CAC'GTC Cuts at: 1318 2329 3213 1318 Size: 1011 884 4226 Fragments arranged by size: 4226 1011 884 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2161 2289 3852 Size: 416 53 83 399 24 744 128 1563 Cuts at: 3852 4810 442 Size: 958 1753 Fragments arranged by size: 1753 1563 958 744 416 399 128 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 3116 3177 4781 1239 Size: 833 1044 61 1604 2579 Fragments arranged by size: 2579 1604 1044 833 61 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4246 5111 5409 4246 Size: 865 298 4958 Fragments arranged by size: 4958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2533 3022 4784 882 Size: 617 1034 489 1762 2219 Fragments arranged by size: 2219 1762 1034 617 489 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2466 3928 4310 4945 Size: 53 83 186 936 933 1462 382 635 Cuts at: 4945 275 Size: 1451 Fragments arranged by size: 1462 1451 936 933 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4554 4952 5226 5373 5784 4554 Size: 398 274 147 411 4891 Fragments arranged by size: 4891 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3069 3099 1178 Size: 30 165 30 1666 30 4200 Fragments arranged by size: 4200 1666 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2296 1186 Size: 1110 5011 BseYI C'CCAG_C Cuts at: 3117 3361 5324 5820 3117 Size: 244 1963 496 3418 Fragments arranged by size: 3418 1963 496 244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 2538 2741 2756 1163 Size: 573 606 196 203 15 4528 Fragments arranged by size: 4528 606 573 203 196 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2962 3571 4332 5360 1540 Size: 275 186 961 609 761 1028 2301 Fragments arranged by size: 2301 1028 961 761 609 275 186 BsiHKAI G_wGCw'C Cuts at: 3381 4182 4267 5338 3381 Size: 801 85 1071 4164 Fragments arranged by size: 4164 1071 801 85 BsmI GAATG_Cn' Cuts at: 3632 3725 3632 Size: 93 6028 BsmBI CGTCTCn'nnnn_ Cuts at: 2457 2504 2457 Size: 47 6074 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2336 2343 2350 2376 2886 3129 3354 Size: 399 546 7 7 26 510 243 225 Cuts at: 3354 3381 3440 4182 4267 5338 1391 Size: 27 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 510 399 243 225 85 59 27 26 7 7 BspEI T'CCGG_A Cuts at: 5784 5784 Size: 6121 BspHI T'CATG_A Cuts at: 2598 4010 5740 2598 Size: 1412 1730 2979 Fragments arranged by size: 2979 1730 1412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 2249 3271 3476 844 Size: 1253 152 1022 205 3489 Fragments arranged by size: 3489 1253 1022 205 152 BsrBI CCG'CTC Cuts at: 1394 1823 4008 1394 Size: 429 2185 3507 Fragments arranged by size: 3507 2185 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2482 4616 4790 65 Size: 1183 1234 2134 174 1396 Fragments arranged by size: 2134 1396 1234 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2187 4765 4952 1700 Size: 110 377 2578 187 2869 Fragments arranged by size: 2869 2578 377 187 110 BsrGI T'GTAC_A Cuts at: 96 3219 96 Size: 3123 2998 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 2250 794 Size: 1456 4665 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6121 BstXI CCAn_nnnn'nTGG Cuts at: 2050 3331 2050 Size: 1281 4840 BstYI r'GATC_y Cuts at: 1972 2760 3451 3818 4203 4220 5672 5758 5770 Size: 788 691 367 385 17 1452 86 12 Cuts at: 5770 6116 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 788 691 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2999 2999 Size: 6121 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4630 Fragments arranged by size: 4630 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 2422 3637 4431 4451 2422 Size: 1215 794 20 4092 Fragments arranged by size: 4092 1215 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DraIII CAC_nnn'GTG Cuts at: 2613 2613 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2430 3568 Size: 54 1425 50 275 18 306 294 1138 Cuts at: 3568 4457 6086 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1138 889 306 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 3056 4051 1353 Size: 717 986 995 3423 Fragments arranged by size: 3423 995 986 717 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3379 3379 Size: 6121 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2228 2959 3116 3177 3332 4184 Size: 393 440 156 731 157 61 155 852 Cuts at: 4184 4781 5568 5836 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 852 787 731 597 440 393 268 157 156 155 61 EcoO109I rG'GnC_Cy Cuts at: 2066 2122 2347 2995 3080 3161 2066 Size: 56 225 648 85 81 5026 Fragments arranged by size: 5026 648 225 85 81 56 EcoRI G'AATT_C Cuts at: 3430 3808 3430 Size: 378 5743 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2762 2762 Size: 6121 FspI TGC'GCA Cuts at: 2809 4627 2809 Size: 1818 4303 HaeII r_GCGC'y Cuts at: 1678 2710 2833 3199 5268 1678 Size: 1032 123 366 2069 2531 Fragments arranged by size: 2531 2069 1032 366 123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2144 2176 2318 2350 2580 Size: 32 135 32 572 32 142 32 230 Cuts at: 2580 2612 2716 2748 3021 3053 3389 3421 4762 Size: 32 104 32 273 32 336 32 1341 Cuts at: 4762 4794 4836 4868 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1341 572 336 273 230 142 135 104 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2152 3465 3711 669 Size: 988 281 214 1313 246 3079 Fragments arranged by size: 3079 1313 988 281 246 214 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 2581 3446 2581 Size: 865 5256 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5235 5419 1305 Size: 3930 184 2007 Fragments arranged by size: 3930 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2138 2432 10 Size: 54 1425 343 306 294 3699 Fragments arranged by size: 3699 1425 343 306 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 2688 4079 4438 4597 518 Size: 648 1152 370 1391 359 159 2042 Fragments arranged by size: 2042 1391 1152 648 370 359 159 MspA1I CmG'CkG Cuts at: 1606 2250 2436 2654 3537 4214 5362 5607 1606 Size: 644 186 218 883 677 1148 245 2120 Fragments arranged by size: 2120 1148 883 677 644 245 218 186 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4630 Fragments arranged by size: 4630 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 3479 5024 1132 Size: 97 2250 1545 2229 Fragments arranged by size: 2250 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2070 2458 3389 1348 Size: 722 388 931 4080 Fragments arranged by size: 4080 931 722 388 PciI A'CATG_T Cuts at: 5020 5020 Size: 6121 PflMI CCAn_nnn'nTGG Cuts at: 1108 2428 1108 Size: 1320 4801 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2066 2995 2066 Size: 929 5192 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 3691 3691 Size: 6121 PspOMI G'GGCC_C Cuts at: 2346 2346 Size: 6121 PstI C_TGCA'G Cuts at: 830 2246 3282 3473 830 Size: 1416 1036 191 3478 Fragments arranged by size: 3478 1416 1036 191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 2250 2250 Size: 6121 SacI G_AGCT'C Cuts at: 3381 3381 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SapI GCTCTTCn'nnn_ Cuts at: 3056 3056 Size: 6121 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 2242 3278 3469 4604 5285 5476 826 Size: 219 1197 1036 191 1135 681 191 1471 Fragments arranged by size: 1471 1197 1135 1036 681 219 191 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 2345 3448 1872 Size: 473 1103 4545 Fragments arranged by size: 4545 1103 473 SmlI C'TyrA_G Cuts at: 820 1017 1943 4225 5126 5388 820 Size: 197 926 2282 901 262 1553 Fragments arranged by size: 2282 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 3479 1132 Size: 2347 3774 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 2928 1517 Size: 1411 4710 StyI C'CwwG_G Cuts at: 513 1065 2004 2376 2754 2791 3355 3560 513 Size: 552 939 372 378 37 564 205 3074 Fragments arranged by size: 3074 939 564 552 378 372 205 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4148 4165 4318 4503 4948 325 Size: 1255 2568 17 153 185 445 1498 Fragments arranged by size: 2568 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3219 4367 6079 Size: 274 80 33 51 1443 1242 1148 1712 Cuts at: 6079 96 Size: 138 Fragments arranged by size: 1712 1443 1242 1148 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3069 3101 3135 3167 6081 6113 3069 Size: 32 34 32 2914 32 3077 Fragments arranged by size: 3077 2914 34 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2728 1316 Size: 1412 4709 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2398 2425 3331 2398 Size: 27 906 5188 Fragments arranged by size: 5188 906 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 2343 3446 1870 Size: 473 1103 4545 Fragments arranged by size: 4545 1103 473 XmnI GAAnn'nnTTC Cuts at: 4250 4250 Size: 6121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2467 3929 276 Size: 53 83 186 936 933 1462 2468 Fragments arranged by size: 2468 1462 936 933 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI