Restriction map for
pFN21AB4623
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaHI BsiEI BsiWI BsmBI BsoBI
BspDI BspHI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII Cfr10I ClaI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
MfeI MluI MseI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 52 CGCG CG^CG
AccIII 851 TCCGGA T^CCGGA
AflIII 1176 ACACGT A^C[GA][CT]GT
BalI 983 TGGCCA TGG^CCA
BamHI 431 GGATCC G^GATCC
BanI 166 GGTGCC G^G[CT][GA]CC
BanII 1462 GAGCTC G[GA]GC[CT]^C
BbsI 769 GAAGAC GAAGAC(2/6)
BcnI 26 CCGGG CC^[GC]GG
BfaI 1195 CTAG C^TAG
BglI 634 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1083 AGATCT A^GATCT
BpuAI 769 GAAGAC GAAGAC(2/6)
BsaBI 1078 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 333 GGTCTC GGTCTC(1/5)
BsaWI 851 TCCGGA [AT]^CCGG[AT]
BseAI 851 TCCGGA T^CCGGA
BsgI 437 GTGCAG GTGCAG(16/14)
BsmAI 334 GTCTC GTCTC(1/5)
BsmFI 484 GGGAC GGGAC(10/14)
BsmI 1326 GCATTC GAATGC(1/-1)
BspEI 851 TCCGGA T^CCGGA
BspMI 1206 GCAGGT ACCTGC(4/8)
BsrBI 926 CCGCTC CCGCTC(-3/-3)
BstUI 52 CGCG CG^CG
CfoI 41 GCGC GCG^C
CpoI 644 CGGACCG CG^G[AT]CCG
DdeI 785 CTCAG C^T[ACGT]AG
DraII 484 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 983 TGGCCA [CT]^GGCC[GA]
EcoNI 804 CCTTATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 484 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 203 GAATTC G^AATTC
EcoT14I 1319 CCATGG C^C[AT][AT]GG
HhaI 41 GCGC GCG^C
HinP1I 41 GCGC G^CGC
KspI 51 CCGCGG CCGC^GG
MaeI 1195 CTAG C^TAG
MamI 1078 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 983 TGGCCA TGG^CCA
MroI 851 TCCGGA T^CCGGA
MscI 983 TGGCCA TGG^CCA
MslI 1536 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 51 CCGCGG C[AC]G^C[GT]G
MvnI 52 CGCG CG^CG
NciI 26 CCGGG CC^[GC]GG
NcoI 1319 CCATGG C^CATGG
NdeI 341 CATATG CA^TATG
NspI 947 GCATGT [GA]CATG^[CT]
PflMI 1375 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1452 GACTC GAGTC(4/5)
PpuMI 484 GGGACCC [GA]G^G[AT]CC[CT]
RsrII 644 CGGACCG CG^G[AT]CCG
SacI 1462 GAGCTC GAGCT^C
SacII 51 CCGCGG CCGC^GG
SexAI 67 ACCTGGT A^CC[AT]GGT
SmlI 931 CTTGAG C^T[CT][GA]AG
StuI 1473 AGGCCT AGG^CCT
StyI 1319 CCATGG C^C[AT][AT]GG
TaqI 1181 TCGA T^CGA
TthHB8I 1181 TCGA T^CGA
Van91I 1375 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 104 GTGCTC G[AT]GC[AT]^C
1462 GAGCTC
BbvI 1545 GCAGC GCAGC(8/12)
709 GCTGC
BseRI 16 CTCCTC GAGGAG(10/8)
21 CTCCTC
BsiHKAI 104 GTGCTC G[AT]GC[AT]^C
1462 GAGCTC
Bsp1407I 795 TGTACA T^GTACA
1366 TGTACA
BsrGI 795 TGTACA T^GTACA
1366 TGTACA
BstEII 1222 GGTGACC G^GT[ACGT]ACC
1314 GGTCACC
BstPI 1222 GGTGACC G^GT[ACGT]ACC
1314 GGTCACC
BstXI 170 CCACGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1304 CCAAAGTGGTGG
EarI 336 CTCTTC CTCTTC(1/4)
1497 GAAGAG
EcoO65I 1222 GGTGACC G^GT[ACGT]ACC
1314 GGTCACC
FauI 50 CCCGC CCCGC(4/6)
443 GCGGG
HapII 26 CCGG C^CGG
852 CCGG
HpaII 26 CCGG C^CGG
852 CCGG
Ksp632I 336 CTCTTC CTCTTC(1/4)
1497 GAAGAG
MspI 26 CCGG C^CGG
852 CCGG
PstI 257 CTGCAG CTGCA^G
710 CTGCAG
ScaI 374 AGTACT AGT^ACT
1501 AGTACT
SfcI 257 CTGCAG C^T[GA][CT]AG
710 CTGCAG
SspBI 795 TGTACA T^GTACA
1366 TGTACA
TseI 709 GCTGC G^C[AT]GC
1545 GCAGC
Tsp45I 1223 GTGAC ^GT[GC]AC
1315 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DpnII DsaI EcoRII Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tsp509I
TspRI XhoII