(Circular) (Six-base) MAPSORT of: pFN21AB4623.seq Check: 6579 from: 1 to: 6265 pFN21AB4623 6265 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4328 5712 5980 1632 Size: 2696 1384 268 1917 Fragments arranged by size: 2696 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 3181 5164 6141 1203 Size: 1978 1983 977 1327 Fragments arranged by size: 1983 1978 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 4008 5580 4008 Size: 1572 4693 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 2208 2366 2689 3574 3800 3952 6061 1493 Size: 715 158 323 885 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 715 323 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3590 1870 Size: 73 1647 4545 Fragments arranged by size: 4545 1647 73 BamHI G'GATC_C Cuts at: 2436 3595 3962 2436 Size: 1159 367 4739 Fragments arranged by size: 4739 1159 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2171 3586 5041 618 Size: 325 217 627 384 1415 1455 1842 Fragments arranged by size: 1842 1455 1415 627 384 325 217 BanII G_rGCy'C Cuts at: 1391 3471 3584 1391 Size: 2080 113 4072 Fragments arranged by size: 4072 2080 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2781 928 Size: 971 882 4412 Fragments arranged by size: 4412 971 882 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2645 4876 Size: 107 122 71 631 605 337 636 2231 Cuts at: 4876 136 Size: 1525 Fragments arranged by size: 2231 1525 636 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3088 6260 3088 Size: 3172 3093 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 2423 4326 5482 1790 Size: 633 1903 1156 2573 Fragments arranged by size: 2573 1903 1156 633 BmgBI CAC'GTC Cuts at: 1318 3184 1318 Size: 1866 4399 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 3996 4954 Size: 416 53 83 399 24 2102 477 958 Cuts at: 4954 442 Size: 1753 Fragments arranged by size: 2102 1753 958 477 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2949 2949 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2265 2475 2754 2916 3130 4925 1239 Size: 1026 210 279 162 214 1795 2579 Fragments arranged by size: 2579 1795 1026 279 214 210 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2957 4390 5255 5553 2957 Size: 1433 865 298 3669 Fragments arranged by size: 3669 1433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2344 4928 882 Size: 617 845 2584 2219 Fragments arranged by size: 2584 2219 845 617 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 3087 6259 3087 Size: 3172 3093 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2856 4698 5096 5370 5517 5928 2856 Size: 1842 398 274 147 411 3193 Fragments arranged by size: 3193 1842 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2889 2919 1178 Size: 30 165 30 1486 30 4524 Fragments arranged by size: 4524 1486 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2017 1186 Size: 826 5 5434 Fragments arranged by size: 5434 826 5 BseYI C'CCAG_C Cuts at: 5468 5964 5468 Size: 496 5769 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2463 1163 Size: 573 727 4965 Fragments arranged by size: 4965 727 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2113 3471 4326 4411 5482 2113 Size: 1358 855 85 1071 2896 Fragments arranged by size: 2896 1358 1071 855 85 BsmI GAATG_Cn' Cuts at: 3331 3776 3869 3331 Size: 445 93 5727 Fragments arranged by size: 5727 445 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2423 3471 3584 4326 4411 5482 Size: 399 323 310 1048 113 742 85 1071 Cuts at: 5482 1391 Size: 2174 Fragments arranged by size: 2174 1071 1048 742 399 323 310 113 85 BspEI T'CCGG_A Cuts at: 2856 5928 2856 Size: 3072 3193 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3202 3620 844 Size: 2358 418 3489 Fragments arranged by size: 3489 2358 418 BsrBI CCG'CTC Cuts at: 1394 1823 2933 4152 1394 Size: 429 1110 1219 3507 Fragments arranged by size: 3507 1219 1110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2547 3171 3401 4760 4934 65 Size: 1183 1299 624 230 1359 174 1396 Fragments arranged by size: 1396 1359 1299 1183 624 230 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4909 5096 1700 Size: 110 3099 187 2869 Fragments arranged by size: 3099 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2800 3371 96 Size: 2704 571 2990 Fragments arranged by size: 2990 2704 571 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 3227 3319 1184 Size: 2043 92 4130 Fragments arranged by size: 4130 2043 92 BstXI CCAn_nnnn'nTGG Cuts at: 2182 3316 2182 Size: 1134 5131 BstYI r'GATC_y Cuts at: 1972 2060 2137 2436 3088 3595 3962 4347 4364 Size: 88 77 299 652 507 367 385 17 Cuts at: 4364 5816 5902 5914 6260 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 652 507 385 367 346 299 88 86 77 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2056 2175 3324 513 Size: 552 939 52 119 1149 3454 Fragments arranged by size: 3454 1149 939 552 119 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2099 2789 3165 3221 487 Size: 683 56 873 690 376 56 3531 Fragments arranged by size: 3531 873 690 683 376 56 56 BtsI GCAGTG_nn' Cuts at: 2545 3781 4575 4595 2545 Size: 1236 794 20 4215 Fragments arranged by size: 4215 1236 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2988 3712 4601 Size: 54 1425 50 275 18 1158 724 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1158 889 724 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2347 3497 4195 1353 Size: 994 1150 698 3423 Fragments arranged by size: 3423 1150 994 698 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2663 4852 5226 5372 387 Size: 1064 138 1074 2189 374 146 1280 Fragments arranged by size: 2189 1280 1074 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3469 3469 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 2475 2754 2916 3130 4328 4925 Size: 393 633 210 279 162 214 1198 597 Cuts at: 4925 5712 5980 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1198 787 633 597 393 279 268 214 210 162 EcoNI CCTnn'n_nnAGG Cuts at: 2813 2813 Size: 6265 EcoO109I rG'GnC_Cy Cuts at: 2490 2490 Size: 6265 EcoRI G'AATT_C Cuts at: 2208 3574 3952 2208 Size: 1366 378 4521 Fragments arranged by size: 4521 1366 378 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 5412 1678 Size: 3734 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2502 2534 2834 2866 3278 Size: 32 135 32 930 32 300 32 412 Cuts at: 3278 3310 4906 4938 4980 5012 1373 Size: 32 1596 32 42 32 2626 Fragments arranged by size: 2626 1596 930 412 300 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 3403 3456 3609 3855 669 Size: 988 281 349 1116 53 153 246 3079 Fragments arranged by size: 3079 1116 988 349 281 246 153 53 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5379 5563 1305 Size: 4074 184 2007 Fragments arranged by size: 4074 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2990 10 Size: 54 1425 343 1158 3285 Fragments arranged by size: 3285 1425 1158 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3545 4223 4582 4741 518 Size: 648 2379 678 359 159 2042 Fragments arranged by size: 2379 2042 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 3681 4358 5506 5751 1606 Size: 452 1623 677 1148 245 2120 Fragments arranged by size: 2120 1623 1148 677 452 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3324 513 Size: 552 939 1320 3454 Fragments arranged by size: 3454 1320 939 552 NdeI CA'TA_TG Cuts at: 387 2347 387 Size: 1960 4305 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2956 3623 5168 1132 Size: 97 1727 667 1545 2229 Fragments arranged by size: 2229 1727 1545 667 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 3386 1108 Size: 2278 3987 PfoI T'CCnGG_A Cuts at: 3127 3127 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2490 2490 Size: 6265 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 2813 3835 2813 Size: 1022 5243 PstI C_TGCA'G Cuts at: 830 2266 2719 3617 830 Size: 1436 453 898 3478 Fragments arranged by size: 3478 1436 898 453 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 RsrII CG'GwC_CG Cuts at: 2650 2650 Size: 6265 SacI G_AGCT'C Cuts at: 3471 3471 Size: 6265 SacII CC_GC'GG Cuts at: 2059 2059 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SanDI GG'GwC_CC Cuts at: 2490 2490 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 2381 3508 4513 6225 2381 Size: 1127 1005 1712 2421 Fragments arranged by size: 2421 1712 1127 1005 SexAI A'CCwGG_T Cuts at: 2072 2072 Size: 6265 SfcI C'TryA_G Cuts at: 826 1045 2262 2715 3613 4748 5429 5620 826 Size: 219 1217 453 898 1135 681 191 1471 Fragments arranged by size: 1471 1217 1135 898 681 453 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2936 4369 5270 5532 820 Size: 197 926 993 1433 901 262 1553 Fragments arranged by size: 1553 1433 993 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 3480 1517 Size: 1963 4302 StyI C'CwwG_G Cuts at: 513 1065 2004 3324 3704 513 Size: 552 939 1320 380 3074 Fragments arranged by size: 3074 1320 939 552 380 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2304 2667 3501 4292 4309 4462 4647 Size: 1255 724 363 834 791 17 153 185 Cuts at: 4647 5092 325 Size: 445 1498 Fragments arranged by size: 1498 1255 834 791 724 445 363 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 2800 3371 Size: 274 80 33 51 1443 402 421 571 Cuts at: 3371 3506 4511 6223 96 Size: 135 1005 1712 138 Fragments arranged by size: 1712 1443 1005 571 421 402 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII SapI SfiI SfoI SrfI SwaI XcmI