Restriction map for
pFN21AB4623
(Linear) (Six-base) MAPSORT of: KIBB4623.seq Check: 8558 from: 1 to: 1560
KIBB4623.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AflIII A'CryG_T
Cuts at: 0 1176 1560
Size: 1176 384
ApoI r'AATT_y
Cuts at: 0 203 361 684 1560
Size: 203 158 323 876
Fragments arranged by size:
876 323 203 158
BamHI G'GATC_C
Cuts at: 0 431 1560
Size: 431 1129
BanI G'GyrC_C
Cuts at: 0 166 1560
Size: 166 1394
BanII G_rGCy'C
Cuts at: 0 1466 1560
Size: 1466 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 776 1560
Size: 776 784
BglI GCCn_nnn'nGGC
Cuts at: 0 640 1560
Size: 640 920
BglII A'GATC_T
Cuts at: 0 1083 1560
Size: 1083 477
Bme1580I G_kGCm'C
Cuts at: 0 418 1560
Size: 418 1142
BmgBI CAC'GTC
Cuts at: 0 1179 1560
Size: 1179 381
BmrI ACTGGGnnnn_n'
Cuts at: 0 1514 1560
Size: 1514 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 944 1560
Size: 944 616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 470 749 911 1125 1560
Size: 260 210 279 162 214 435
Fragments arranged by size:
435 279 260 214 210 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1560
Size: 952 608
BsaI GGTCTCn'nnnn_
Cuts at: 0 339 1560
Size: 339 1221
BsaBI GATnn'nnATC
Cuts at: 0 1082 1560
Size: 1082 478
BsaWI w'CCGG_w
Cuts at: 0 851 1560
Size: 851 709
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 884 914 1560
Size: 884 30 646
Fragments arranged by size:
884 646 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 12 1560
Size: 7 5 1548
Fragments arranged by size:
1548 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1560
Size: 458 1102
BsiHKAI G_wGCw'C
Cuts at: 0 108 1466 1560
Size: 108 1358 94
Fragments arranged by size:
1358 108 94
BsmI GAATG_Cn'
Cuts at: 0 1326 1560
Size: 1326 234
Bsp1286I G_dGCh'C
Cuts at: 0 108 418 1466 1560
Size: 108 310 1048 94
Fragments arranged by size:
1048 310 108 94
BspEI T'CCGG_A
Cuts at: 0 851 1560
Size: 851 709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1197 1560
Size: 1197 363
BsrBI CCG'CTC
Cuts at: 0 928 1560
Size: 928 632
BsrDI GCAATG_nn'
Cuts at: 0 542 1166 1396 1560
Size: 542 624 230 164
Fragments arranged by size:
624 542 230 164
BsrGI T'GTAC_A
Cuts at: 0 795 1366 1560
Size: 795 571 194
Fragments arranged by size:
795 571 194
BstEII G'GTnAC_C
Cuts at: 0 1222 1314 1560
Size: 1222 92 246
Fragments arranged by size:
1222 246 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 1311 1560
Size: 177 1134 249
Fragments arranged by size:
1134 249 177
BstYI r'GATC_y
Cuts at: 0 55 132 431 1083 1560
Size: 55 77 299 652 477
Fragments arranged by size:
652 477 299 77 55
BtgI C'CryG_G
Cuts at: 0 51 170 1319 1560
Size: 51 119 1149 241
Fragments arranged by size:
1149 241 119 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 94 784 1160 1216 1560
Size: 94 690 376 56 344
Fragments arranged by size:
690 376 344 94 56
BtsI GCAGTG_nn'
Cuts at: 0 540 1560
Size: 540 1020
EaeI y'GGCC_r
Cuts at: 0 983 1560
Size: 983 577
EarI CTCTTCn'nnn_
Cuts at: 0 342 1492 1560
Size: 342 1150 68
Fragments arranged by size:
1150 342 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 658 1560
Size: 658 902
EcoICRI GAG'CTC
Cuts at: 0 1464 1560
Size: 1464 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 470 749 911 1125 1560
Size: 260 210 279 162 214 435
Fragments arranged by size:
435 279 260 214 210 162
EcoNI CCTnn'n_nnAGG
Cuts at: 0 808 1560
Size: 808 752
EcoO109I rG'GnC_Cy
Cuts at: 0 485 1560
Size: 485 1075
EcoRI G'AATT_C
Cuts at: 0 203 1560
Size: 203 1357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 497 529 829 861 1273 1305 1560
Size: 497 32 300 32 412 32 255
Fragments arranged by size:
497 412 300 255 32 32 32
HincII GTy'rAC
Cuts at: 0 282 1398 1451 1560
Size: 282 1116 53 109
Fragments arranged by size:
1116 282 109 53
MscI TGG'CCA
Cuts at: 0 985 1560
Size: 985 575
MslI CAynn'nnrTG
Cuts at: 0 1540 1560
Size: 1540 20
MspA1I CmG'CkG
Cuts at: 0 53 1560
Size: 53 1507
NcoI C'CATG_G
Cuts at: 0 1319 1560
Size: 1319 241
NdeI CA'TA_TG
Cuts at: 0 342 1560
Size: 342 1218
NspI r_CATG'y
Cuts at: 0 951 1560
Size: 951 609
PflMI CCAn_nnn'nTGG
Cuts at: 0 1381 1560
Size: 1381 179
PfoI T'CCnGG_A
Cuts at: 0 1122 1560
Size: 1122 438
PpuMI rG'GwC_Cy
Cuts at: 0 485 1560
Size: 485 1075
PsiI TTA'TAA
Cuts at: 0 808 1560
Size: 808 752
PstI C_TGCA'G
Cuts at: 0 261 714 1560
Size: 261 453 846
Fragments arranged by size:
846 453 261
RsrII CG'GwC_CG
Cuts at: 0 645 1560
Size: 645 915
SacI G_AGCT'C
Cuts at: 0 1466 1560
Size: 1466 94
SacII CC_GC'GG
Cuts at: 0 54 1560
Size: 54 1506
SanDI GG'GwC_CC
Cuts at: 0 485 1560
Size: 485 1075
ScaI AGT'ACT
Cuts at: 0 376 1503 1560
Size: 376 1127 57
Fragments arranged by size:
1127 376 57
SexAI A'CCwGG_T
Cuts at: 0 67 1560
Size: 67 1493
SfcI C'TryA_G
Cuts at: 0 257 710 1560
Size: 257 453 850
Fragments arranged by size:
850 453 257
SmlI C'TyrA_G
Cuts at: 0 931 1560
Size: 931 629
StuI AGG'CCT
Cuts at: 0 1475 1560
Size: 1475 85
StyI C'CwwG_G
Cuts at: 0 1319 1560
Size: 1319 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 299 662 1496 1560
Size: 299 363 834 64
Fragments arranged by size:
834 363 299 64
TatI w'GTAC_w
Cuts at: 0 374 795 1366 1501 1560
Size: 374 421 571 135 59
Fragments arranged by size:
571 421 374 135 59
Enzymes that do cut:
AflIII ApoI BamHI BanI BanII BbsI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI
BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrGI BstEII BstXI BstYI BtgI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII MscI MslI MspA1I NcoI
NdeI NspI PflMI PfoI PpuMI PsiI PstI RsrII
SacI SacII SanDI ScaI SexAI SfcI SmlI StuI
StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI
BcgI BciVI BclI BfrBI BlpI BmtI Bpu10I BsaAI
BsaHI BseYI BsiEI BsiWI BsmBI BspHI BsrFI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI PvuII SalI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI