################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmBI BspHI BssHII BssSI BstEII BstPI BstUI BstXI BstYI Bsu36I CelII CfoI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HpaI KasI KpnI KspI MfeI MflI MluI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccIII 100 TCCGGA T^CCGGA AcyI 512 GACGCC G[GA]^CG[CT]C Asp700I 528 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 477 GAGCTC G[AT]GC[AT]^C AvaI 419 CTCGAG C^[CT]CG[GA]G AvaII 1101 GGACC G^G[AT]CC BalI 263 TGGCCA TGG^CCA BcnI 7 CCCGG CC^[GC]GG BglI 523 GCCTTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 335 CTCCAG CTGGAG(16/14) BsaBI 274 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 512 GACGCC G[GA]^CG[CT]C BsaI 1247 GAGACC GGTCTC(1/5) BseAI 100 TCCGGA T^CCGGA BseRI 352 GAGGAG GAGGAG(10/8) BsgI 1359 CTGCAC GTGCAG(16/14) BsiHKAI 477 GAGCTC G[AT]GC[AT]^C BsmI 532 GCATTC GAATGC(1/-1) BsoBI 419 CTCGAG C^[CT]CG[GA]G Bsp1407I 656 TGTACA T^GTACA BspDI 1471 ATCGAT AT^CGAT BspEI 100 TCCGGA T^CCGGA BspLU11I 798 ACATGT A^CATGT BsrBI 74 CCGCTC CCGCTC(-3/-3) BsrFI 43 ACCGGC [GA]^CCGG[CT] BsrGI 656 TGTACA T^GTACA Bst1107I 1352 GTATAC GTA^TAC BstBI 1133 TTCGAA TT^CGAA BstZ17I 1352 GTATAC GTA^TAC Cfr10I 43 ACCGGC [GA]^CCGG[CT] Cfr13I 1101 GGACC G^G[ACGT]CC ClaI 1471 ATCGAT AT^CGAT DraI 1432 TTTAAA TTT^AAA DraIII 833 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 464 CCACGG C^C[GA][CT]GG EcoRI 827 GAATTC G^AATTC EcoT14I 500 CCTTGG C^C[AT][AT]GG HgaI 512 GACGC GACGC(5/10) Hin1I 512 GACGCC G[GA]^CG[CT]C HindIII 673 AAGCTT A^AGCTT MamI 274 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 263 TGGCCA TGG^CCA MroI 100 TCCGGA T^CCGGA MscI 263 TGGCCA TGG^CCA MslI 465 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 7 CCCGG CC^[GC]GG NdeI 905 CATATG CA^TATG NspI 798 ACATGT [GA]CATG^[CT] NspV 1133 TTCGAA TT^CGAA PaeR7I 419 CTCGAG C^TCGAG PleI 1234 GAGTC GAGTC(4/5) PshAI 1340 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 477 GAGCTC GAGCT^C SalI 1183 GTCGAC G^TCGAC SapI 1764 GCTCTTC GCTCTTC(1/4) Sau96I 1101 GGACC G^G[ACGT]CC SfuI 1133 TTCGAA TT^CGAA SspBI 656 TGTACA T^GTACA SspI 238 AATATT AAT^ATT StuI 1718 AGGCCT AGG^CCT StyI 500 CCTTGG C^C[AT][AT]GG XcmI 851 CCAAAGTCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 419 CTCGAG C^TCGAG XmnI 528 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 55 GAATTT [GA]^AATT[CT] 827 GAATTC AflIII 189 ACGTGT A^C[GA][CT]GT 798 ACATGT ApoI 55 GAATTT [GA]^AATT[CT] 827 GAATTC BanII 477 GAGCTC G[GA]GC[CT]^C 698 GAGCCC BbsI 936 GTCTTC GAAGAC(2/6) 1148 GTCTTC BfaI 229 CTAG C^TAG 509 CTAG BmyI 477 GAGCTC G[AGT]GC[ACT]^C 698 GAGCCC BpuAI 936 GTCTTC GAAGAC(2/6) 1148 GTCTTC BsaWI 100 TCCGGA [AT]^CCGG[AT] 1687 TCCGGT BsiYI 1092 CCTTCAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1307 CCAAGAACGGG BslI 1092 CCTTCAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1307 CCAAGAACGGG Bsp1286I 477 GAGCTC G[AGT]GC[ACT]^C 698 GAGCCC BspMI 811 GCAGGT ACCTGC(4/8) 1317 GCAGGT BstAPI 211 GCAGTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 811 GCAGGTGCTGC DpnII 871 GATC ^GATC 1474 GATC EaeI 9 CGGCCA [CT]^GGCC[GA] 263 TGGCCA HincII 159 GTTGAC GT[CT]^[GA]AC 1183 GTCGAC HindII 159 GTTGAC GT[CT]^[GA]AC 1183 GTCGAC HphI 747 GGTGA GGTGA(8/7) 946 GGTGA MaeI 229 CTAG C^TAG 509 CTAG MaeII 189 ACGT A^CGT 1640 ACGT MboI 871 GATC ^GATC 1474 GATC MseI 1156 TTAA T^TAA 1433 TTAA NdeII 871 GATC ^GATC 1474 GATC PvuII 652 CAGCTG CAG^CTG 742 CAGCTG Sau3AI 871 GATC ^GATC 1474 GATC Tru9I 1156 TTAA T^TAA 1433 TTAA Tsp45I 424 GTGAC ^GT[GC]AC 1483 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AccI AciI AfaI AluI AlwNI BbvI BsaJI BsmAI BsmFI BsrDI BsrI BssKI BstF5I BstNI Cac8I DdeI EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeIII MboII MnlI MspA1I MspI MvaI MwoI NlaIII NlaIV PstI RsaI ScrFI SfaNI SfcI SmlI TaqI TfiI TseI Tsp509I TspRI TthHB8I