(Circular) (Six-base) MAPSORT of: pFN21AE1522.seq Check: 2071 from: 1 to: 6481 pFN21AE1522 6481 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2807 2807 Size: 6481 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3189 3358 3648 3824 1937 Size: 1252 169 290 176 4594 Fragments arranged by size: 4594 1252 290 176 169 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3083 3621 4544 5928 6196 1632 Size: 1451 538 923 1384 268 1917 Fragments arranged by size: 1917 1451 1384 923 538 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 2194 2803 5380 6357 1203 Size: 991 609 2577 977 1327 Fragments arranged by size: 2577 1327 991 977 609 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 5210 5210 Size: 6481 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6481 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6481 AlwNI CAG_nnn'CTG Cuts at: 2789 2822 3242 4224 5796 2789 Size: 33 420 982 1572 3474 Fragments arranged by size: 3474 1572 982 420 33 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 2060 2832 3790 4016 4168 6277 1493 Size: 567 772 958 226 152 2109 1697 Fragments arranged by size: 2109 1697 958 772 567 226 152 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 2424 3806 1870 Size: 73 481 1382 4545 Fragments arranged by size: 4545 1382 481 73 BamHI G'GATC_C Cuts at: 3811 4178 3811 Size: 367 6114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3802 5257 618 Size: 325 217 627 2015 1455 1842 Fragments arranged by size: 2015 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2486 2707 3800 1391 Size: 1095 221 1093 4072 Fragments arranged by size: 4072 1095 1093 221 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2934 3146 928 Size: 971 1035 212 4263 Fragments arranged by size: 4263 1035 971 212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4672 4706 1903 Size: 34 2735 34 3678 Fragments arranged by size: 3678 2735 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4373 5583 4373 Size: 1210 5271 BclI T'GATC_A Cuts at: 1548 1548 Size: 6481 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2534 5092 136 Size: 107 122 71 631 605 862 2558 1525 Fragments arranged by size: 2558 1525 862 631 605 122 107 71 BglII A'GATC_T Cuts at: 6476 6476 Size: 6481 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 4542 5698 1790 Size: 2752 1156 2573 Fragments arranged by size: 2752 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2195 1318 Size: 877 5604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3746 4212 5170 Size: 416 53 83 399 24 2329 466 958 Cuts at: 5170 442 Size: 1753 Fragments arranged by size: 2329 1753 958 466 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2325 5141 1239 Size: 1086 2816 2579 Fragments arranged by size: 2816 2579 1086 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2641 2747 4606 5471 5769 2641 Size: 106 1859 865 298 3353 Fragments arranged by size: 3353 1859 865 298 106 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3246 5144 882 Size: 617 1747 1898 2219 Fragments arranged by size: 2219 1898 1747 617 BsaAI yAC'GTr Cuts at: 493 1204 4272 493 Size: 711 3068 2702 Fragments arranged by size: 3068 2702 711 BsaBI GATnn'nnATC Cuts at: 2283 6475 2283 Size: 4192 2289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2518 4288 4670 5305 Size: 53 83 186 936 985 1770 382 635 Cuts at: 5305 275 Size: 1451 Fragments arranged by size: 1770 1451 985 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2105 3692 4914 5312 5586 5733 6144 2105 Size: 1587 1222 398 274 147 411 2442 Fragments arranged by size: 2442 1587 1222 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6256 Fragments arranged by size: 6256 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 1186 Size: 1186 5295 BseYI C'CCAG_C Cuts at: 2655 2812 5684 6180 2655 Size: 157 2872 496 2956 Fragments arranged by size: 2956 2872 496 157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3349 1163 Size: 573 1613 4295 Fragments arranged by size: 4295 1613 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3931 4692 5720 1540 Size: 275 186 1930 761 1028 2301 Fragments arranged by size: 2301 1930 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2486 4542 4627 5698 2486 Size: 2056 85 1071 3269 Fragments arranged by size: 3269 2056 1071 85 BsmI GAATG_Cn' Cuts at: 2537 3992 4085 2537 Size: 1455 93 4933 Fragments arranged by size: 4933 1455 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2486 2707 3800 4542 4627 5698 1391 Size: 399 696 221 1093 742 85 1071 2174 Fragments arranged by size: 2174 1093 1071 742 696 399 221 85 BspEI T'CCGG_A Cuts at: 2105 6144 2105 Size: 4039 2442 BspHI T'CATG_A Cuts at: 4370 6100 4370 Size: 1730 4751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2807 3313 3836 844 Size: 1963 506 523 3489 Fragments arranged by size: 3489 1963 523 506 BsrBI CCG'CTC Cuts at: 1394 1823 2081 4368 1394 Size: 429 258 2287 3507 Fragments arranged by size: 3507 2287 429 258 BsrDI GCAATG_nn' Cuts at: 65 1248 3214 3270 3678 4976 5150 65 Size: 1183 1966 56 408 1298 174 1396 Fragments arranged by size: 1966 1396 1298 1183 408 174 56 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 5125 5312 1700 Size: 110 238 3077 187 2869 Fragments arranged by size: 3077 2869 238 187 110 BsrGI T'GTAC_A Cuts at: 96 2661 96 Size: 2565 3916 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 4541 5553 4541 Size: 1012 5469 BstAPI GCAn_nnn'nTGC Cuts at: 794 2222 2822 794 Size: 1428 600 4453 Fragments arranged by size: 4453 1428 600 BstBI TT'CG_AA Cuts at: 3139 5285 3139 Size: 2146 4335 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6481 BstYI r'GATC_y Cuts at: 1972 3811 4178 4563 4580 6032 6118 6130 6476 Size: 1839 367 385 17 1452 86 12 346 Cuts at: 6476 1972 Size: 1977 Fragments arranged by size: 1977 1839 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3359 3359 Size: 6481 BtgI C'CryG_G Cuts at: 513 1065 2004 2469 513 Size: 552 939 465 4525 Fragments arranged by size: 4525 939 552 465 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2742 487 Size: 683 56 1516 4226 Fragments arranged by size: 4226 1516 683 56 BtsI GCAGTG_nn' Cuts at: 2666 3361 3997 4791 4811 2666 Size: 695 636 794 20 4336 Fragments arranged by size: 4336 794 695 636 20 ClaI AT'CG_AT Cuts at: 1554 3477 1554 Size: 1923 4558 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 3439 3785 3439 Size: 346 6135 DraIII CAC_nnn'GTG Cuts at: 2843 2843 Size: 6481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2014 2268 3928 Size: 54 1425 50 275 18 184 254 1660 Cuts at: 3928 4817 6446 8 Size: 889 1629 43 Fragments arranged by size: 1660 1629 1425 889 275 254 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3928 1537 Size: 275 2116 4090 Fragments arranged by size: 4090 2116 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3164 3446 3776 4411 1353 Size: 1811 282 330 635 3423 Fragments arranged by size: 3423 1811 635 330 282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5068 5442 5588 387 Size: 1064 138 3479 374 146 1280 Fragments arranged by size: 3479 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2484 2484 Size: 6481 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2325 3083 3621 4544 5141 5928 6196 Size: 393 693 758 538 923 597 787 268 Cuts at: 6196 1239 Size: 1524 Fragments arranged by size: 1524 923 787 758 693 597 538 393 268 EcoRI G'AATT_C Cuts at: 2832 3790 4168 2832 Size: 958 378 5145 Fragments arranged by size: 5145 958 378 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 5628 1678 Size: 3950 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2924 2956 3243 3275 3447 Size: 32 135 32 1352 32 287 32 172 Cuts at: 3447 3479 5122 5154 5196 5228 1373 Size: 32 1643 32 42 32 2626 Fragments arranged by size: 2626 1643 1352 287 172 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2166 3190 3825 4071 669 Size: 988 281 228 1024 635 246 3079 Fragments arranged by size: 3079 1024 988 635 281 246 228 HindIII A'AGCT_T Cuts at: 748 2678 748 Size: 1930 4551 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 4080 4080 Size: 6481 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2809 5595 5779 1305 Size: 1504 2786 184 2007 Fragments arranged by size: 2786 2007 1504 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 10 Size: 54 1425 343 438 4221 Fragments arranged by size: 4221 1425 438 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 4439 4798 4957 518 Size: 648 1308 1965 359 159 2042 Fragments arranged by size: 2042 1965 1308 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2659 2749 2789 2906 3897 4574 5722 5967 Size: 1053 90 40 117 991 677 1148 245 Cuts at: 5967 1606 Size: 2120 Fragments arranged by size: 2120 1148 1053 991 677 245 117 90 40 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4990 Fragments arranged by size: 4990 939 552 NdeI CA'TA_TG Cuts at: 387 2911 387 Size: 2524 3957 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 2807 3839 5384 1132 Size: 97 1578 1032 1545 2229 Fragments arranged by size: 2229 1578 1545 1032 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 2803 5380 2803 Size: 2577 3904 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6481 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6063 6095 4550 Size: 32 1481 32 4936 Fragments arranged by size: 4936 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3349 1613 Size: 1736 4745 PsiI TTA'TAA Cuts at: 2291 2717 4051 2291 Size: 426 1334 4721 Fragments arranged by size: 4721 1334 426 PspXI vC'TCGA_Gb Cuts at: 2424 2424 Size: 6481 PstI C_TGCA'G Cuts at: 830 2218 2410 2673 3833 830 Size: 1388 192 263 1160 3478 Fragments arranged by size: 3478 1388 1160 263 192 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 PvuII CAG'CTG Cuts at: 2659 2749 2659 Size: 90 6391 SacI G_AGCT'C Cuts at: 2486 2486 Size: 6481 SalI G'TCGA_C Cuts at: 1936 3188 3823 1936 Size: 1252 635 4594 Fragments arranged by size: 4594 1252 635 SapI GCTCTTCn'nnn_ Cuts at: 3776 3776 Size: 6481 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 4729 6441 4729 Size: 1712 4769 SfcI C'TryA_G Cuts at: 826 1045 2214 2406 2669 3829 4964 5645 5836 Size: 219 1169 192 263 1160 1135 681 191 Cuts at: 5836 826 Size: 1471 Fragments arranged by size: 1471 1169 1160 1135 681 263 219 192 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2424 2620 2762 4585 5486 5748 Size: 197 926 481 196 142 1823 901 262 Cuts at: 5748 820 Size: 1553 Fragments arranged by size: 1823 1553 926 901 481 262 197 196 142 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SspI AAT'ATT Cuts at: 5 52 2245 4405 5 Size: 47 2193 2160 2081 Fragments arranged by size: 2193 2160 2081 47 StuI AGG'CCT Cuts at: 1517 3725 1517 Size: 2208 4273 StyI C'CwwG_G Cuts at: 513 1065 2004 2505 3920 513 Size: 552 939 501 1415 3074 Fragments arranged by size: 3074 1415 939 552 501 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2911 4508 4525 4678 4863 5308 325 Size: 1255 1331 1597 17 153 185 445 1498 Fragments arranged by size: 1597 1498 1331 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2592 2661 3282 Size: 274 80 33 51 1443 615 69 621 Cuts at: 3282 4727 6439 96 Size: 1445 1712 138 Fragments arranged by size: 1712 1445 1443 621 615 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6441 6473 6441 Size: 32 6449 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6481 XbaI T'CTAG_A Cuts at: 3817 3817 Size: 6481 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2863 2863 Size: 6481 XhoI C'TCGA_G Cuts at: 1943 2424 1943 Size: 481 6000 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 2537 4610 2537 Size: 2073 4408 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI