################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tsp45I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AfaI 819 GTAC GT^AC AocI 745 CCTGAGG CC^T[ACGT]AGG Aor51HI 356 AGCGCT AGC^GCT Asp700I 921 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 195 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 767 CTCGGG C^[CT]CG[GA]G BalI 173 TGGCCA TGG^CCA BbsI 940 GTCTTC GAAGAC(2/6) BglI 175 GCCAACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 940 GTCTTC GAAGAC(2/6) BsaBI 525 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 118 GAGACG CGTCTC(1/5) BsoBI 767 CTCGGG C^[CT]CG[GA]G BspDI 208 ATCGAT AT^CGAT BsrDI 426 CATTGC GCAATG(2/0) BsrFI 215 ACCGGC [GA]^CCGG[CT] BstXI 458 CCATTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 745 CCTGAGG CC^T[ACGT]AGG Cfr10I 215 ACCGGC [GA]^CCGG[CT] ClaI 208 ATCGAT AT^CGAT DraIII 725 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 829 CTCTTC CTCTTC(1/4) Eco47III 356 AGCGCT AGC^GCT Eco81I 745 CCTGAGG CC^T[ACGT]AGG EcoNI 552 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 602 GCGGG CCCGC(4/6) Ksp632I 829 CTCTTC CTCTTC(1/4) MamI 525 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 173 TGGCCA TGG^CCA MscI 173 TGGCCA TGG^CCA MseI 26 TTAA T^TAA MspA1I 760 CAGCTG C[AC]G^C[GT]G NlaIV 257 GGAACC GG[ACGT]^[ACGT]CC PflFI 195 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 724 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 571 GAGTC GAGTC(4/5) PpuMI 512 AGGACCT [GA]G^G[AT]CC[CT] PstI 145 CTGCAG CTGCA^G PvuII 760 CAGCTG CAG^CTG RsaI 819 GTAC GT^AC SapI 828 GCTCTTC GCTCTTC(1/4) SexAI 104 ACCTGGT A^CC[AT]GGT SfcI 145 CTGCAG C^T[GA][CT]AG StuI 560 AGGCCT AGG^CCT TfiI 878 GATTC G^A[AT]TC Tru9I 26 TTAA T^TAA Tsp509I 676 AATT ^AATT Tth111I 195 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 724 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 924 CCAGTTCCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 921 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 327 GATCC GGATC(4/5) 992 GGATC BanII 370 GAGCTC G[GA]GC[CT]^C 445 GAGCTC BcgI 759 GCAGCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 969 GCAGGAGTGTCG BsaJI 74 CCGGGG C^C[ACGT][ACGT]GG 852 CCCTGG BsmAI 118 GAGAC GTCTC(1/5) 857 GAGAC BsmFI 51 GGGAC GGGAC(10/14) 604 GGGAC BspMI 438 ACCTGC ACCTGC(4/8) 841 GCAGGT BssSI 889 CACGAG CACGAG(-5/-1) 373 CTCGTG DraII 512 AGGACCT [GA]G^G[ACGT]CC[CT] 749 AGGGCCT EaeI 173 TGGCCA [CT]^GGCC[GA] 800 TGGCCG EcoO109I 512 AGGACCT [GA]G^G[ACGT]CC[CT] 749 AGGGCCT HaeII 356 AGCGCT [GA]GCGC^[CT] 1013 AGCGCC HgaI 120 GACGC GACGC(5/10) 864 GACGC HinfI 571 GAGTC G^A[ACGT]TC 878 GATTC HphI 187 TCACC GGTGA(8/7) 668 TCACC MaeIII 164 GTAAC ^GT[ACGT]AC 308 GTTAC SacI 370 GAGCTC GAGCT^C 445 GAGCTC SmlI 220 CTCAAG C^T[CT][GA]AG 961 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AluI AspHI AvaII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsgI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeI MaeII MboI MboII MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I