(Linear) (Six-base) MAPSORT of: KIEE4340.seq Check: 7131 from: 1 to: 1053 KIEE4340.orf With 182 enzymes: SgfI * March 22, 2012 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 447 1053 Size: 447 606 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 1053 Size: 88 965 AfeI AGC'GCT Cuts at: 0 358 1053 Size: 358 695 AleI CACnn'nnGTG Cuts at: 0 935 1053 Size: 935 118 AvaI C'yCGr_G Cuts at: 0 767 1053 Size: 767 286 BanII G_rGCy'C Cuts at: 0 374 449 1053 Size: 374 75 604 Fragments arranged by size: 604 374 75 BbsI GAAGACnn'nnnn_ Cuts at: 0 933 1053 Size: 933 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 748 782 958 992 1053 Size: 748 34 176 34 61 Fragments arranged by size: 748 176 61 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 181 1053 Size: 181 872 BlpI GC'TnA_GC Cuts at: 0 82 368 443 1053 Size: 82 286 75 610 Fragments arranged by size: 610 286 82 75 Bme1580I G_kGCm'C Cuts at: 0 380 628 1053 Size: 380 248 425 Fragments arranged by size: 425 380 248 BmrI ACTGGGnnnn_n' Cuts at: 0 607 1053 Size: 607 446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 589 655 875 889 1053 Size: 589 66 220 14 164 Fragments arranged by size: 589 220 164 66 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 946 1053 Size: 205 741 107 Fragments arranged by size: 741 205 107 BsaBI GATnn'nnATC Cuts at: 0 529 1053 Size: 529 524 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 343 373 958 988 1053 Size: 343 30 585 30 65 Fragments arranged by size: 585 343 65 30 30 BseYI C'CCAG_C Cuts at: 0 1010 1053 Size: 1010 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 334 573 860 1053 Size: 130 204 239 287 193 Fragments arranged by size: 287 239 204 193 130 BsiHKAI G_wGCw'C Cuts at: 0 83 154 374 449 890 1053 Size: 83 71 220 75 441 163 Fragments arranged by size: 441 220 163 83 75 71 BsmBI CGTCTCn'nnnn_ Cuts at: 0 112 1053 Size: 112 941 Bsp1286I G_dGCh'C Cuts at: 0 83 154 374 380 449 628 890 1053 Size: 83 71 220 6 69 179 262 163 Fragments arranged by size: 262 220 179 163 83 71 69 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 447 832 1053 Size: 447 385 221 Fragments arranged by size: 447 385 221 BsrDI GCAATG_nn' Cuts at: 0 425 1053 Size: 425 628 BsrFI r'CCGG_y Cuts at: 0 215 1053 Size: 215 838 BssSI C'ACGA_G Cuts at: 0 373 889 1053 Size: 373 516 164 Fragments arranged by size: 516 373 164 BstXI CCAn_nnnn'nTGG Cuts at: 0 465 1053 Size: 465 588 Bsu36I CC'TnA_GG Cuts at: 0 746 1053 Size: 746 307 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 348 1053 Size: 348 705 BtsI GCAGTG_nn' Cuts at: 0 118 1053 Size: 118 935 ClaI AT'CG_AT Cuts at: 0 209 1053 Size: 209 844 DraIII CAC_nnn'GTG Cuts at: 0 730 1053 Size: 730 323 EaeI y'GGCC_r Cuts at: 0 173 800 1053 Size: 173 627 253 Fragments arranged by size: 627 253 173 EarI CTCTTCn'nnn_ Cuts at: 0 835 1053 Size: 835 218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 235 1053 Size: 235 818 EcoICRI GAG'CTC Cuts at: 0 372 447 1053 Size: 372 75 606 Fragments arranged by size: 606 372 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 589 655 875 889 1053 Size: 88 501 66 220 14 164 Fragments arranged by size: 501 220 164 88 66 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 556 1053 Size: 556 497 EcoO109I rG'GnC_Cy Cuts at: 0 513 750 1053 Size: 513 237 303 Fragments arranged by size: 513 303 237 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 846 930 1053 Size: 846 84 123 Fragments arranged by size: 846 123 84 HaeII r_GCGC'y Cuts at: 0 360 1017 1053 Size: 360 657 36 Fragments arranged by size: 657 360 36 MscI TGG'CCA Cuts at: 0 175 1053 Size: 175 878 MslI CAynn'nnrTG Cuts at: 0 266 463 935 1053 Size: 266 197 472 118 Fragments arranged by size: 472 266 197 118 MspA1I CmG'CkG Cuts at: 0 762 1053 Size: 762 291 PflMI CCAn_nnn'nTGG Cuts at: 0 730 1053 Size: 730 323 PfoI T'CCnGG_A Cuts at: 0 11 491 632 1053 Size: 11 480 141 421 Fragments arranged by size: 480 421 141 11 PpuMI rG'GwC_Cy Cuts at: 0 513 1053 Size: 513 540 PstI C_TGCA'G Cuts at: 0 149 1053 Size: 149 904 PvuII CAG'CTG Cuts at: 0 762 1053 Size: 762 291 SacI G_AGCT'C Cuts at: 0 374 449 1053 Size: 374 75 604 Fragments arranged by size: 604 374 75 SapI GCTCTTCn'nnn_ Cuts at: 0 835 1053 Size: 835 218 SexAI A'CCwGG_T Cuts at: 0 104 1053 Size: 104 949 SfcI C'TryA_G Cuts at: 0 145 1053 Size: 145 908 SmlI C'TyrA_G Cuts at: 0 220 961 1053 Size: 220 741 92 Fragments arranged by size: 741 220 92 StuI AGG'CCT Cuts at: 0 562 1053 Size: 562 491 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 404 890 1053 Size: 404 486 163 Fragments arranged by size: 486 404 163 TatI w'GTAC_w Cuts at: 0 818 1053 Size: 818 235 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 343 375 958 990 1053 Size: 343 32 583 32 63 Fragments arranged by size: 583 343 63 32 32 Tth111I GACn'n_nGTC Cuts at: 0 198 1053 Size: 198 855 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 931 1053 Size: 931 122 XmnI GAAnn'nnTTC Cuts at: 0 925 1053 Size: 925 128 Enzymes that do cut: AarI AcuI AfeI AleI AvaI BanII BbsI BcgI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaBI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI Bsu36I BtgZI BtsI ClaI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII MscI MslI MspA1I PflMI PfoI PpuMI PstI PvuII SacI SapI SexAI SfcI SmlI StuI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I BtgI CspCI DraI DrdI EagI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI XbaI XhoI XmaI ZraI