(Linear) (Six-base) MAPSORT of: KIEE4429.seq Check: 4524 from: 1 to: 1335 KIEE4429.orf With 182 enzymes: SgfI * March 22, 2012 18:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1307 1335 Size: 1307 28 Acc65I G'GTAC_C Cuts at: 0 1247 1335 Size: 1247 88 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 554 874 1335 Size: 554 320 461 Fragments arranged by size: 554 461 320 AflIII A'CryG_T Cuts at: 0 995 1335 Size: 995 340 AleI CACnn'nnGTG Cuts at: 0 739 1335 Size: 739 596 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 484 516 1335 Size: 484 32 819 Fragments arranged by size: 819 484 32 AlwNI CAG_nnn'CTG Cuts at: 0 649 943 957 1009 1335 Size: 649 294 14 52 326 Fragments arranged by size: 649 326 294 52 14 ApaI G_GGCC'C Cuts at: 0 1329 1335 Size: 1329 6 ApoI r'AATT_y Cuts at: 0 1285 1335 Size: 1285 50 AvaI C'yCGr_G Cuts at: 0 26 910 1328 1335 Size: 26 884 418 7 Fragments arranged by size: 884 418 26 7 AvrII C'CTAG_G Cuts at: 0 330 1335 Size: 330 1005 BanI G'GyrC_C Cuts at: 0 1247 1335 Size: 1247 88 BanII G_rGCy'C Cuts at: 0 70 884 1329 1335 Size: 70 814 445 6 Fragments arranged by size: 814 445 70 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 862 977 1335 Size: 862 115 358 Fragments arranged by size: 862 358 115 BglI GCCn_nnn'nGGC Cuts at: 0 1157 1335 Size: 1157 178 BglII A'GATC_T Cuts at: 0 752 1224 1335 Size: 752 472 111 Fragments arranged by size: 752 472 111 BlpI GC'TnA_GC Cuts at: 0 433 1335 Size: 433 902 Bme1580I G_kGCm'C Cuts at: 0 1329 1335 Size: 1329 6 BmgBI CAC'GTC Cuts at: 0 996 1335 Size: 996 339 Bpu10I CC'TnA_GC Cuts at: 0 698 1335 Size: 698 637 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1156 1213 1335 Size: 1156 57 122 Fragments arranged by size: 1156 122 57 BsaI GGTCTCn'nnnn_ Cuts at: 0 487 1029 1335 Size: 487 542 306 Fragments arranged by size: 542 487 306 BsaWI w'CCGG_w Cuts at: 0 595 1335 Size: 595 740 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 484 514 532 562 694 724 871 901 Size: 484 30 18 30 132 30 147 30 Cuts at: 901 1335 Size: 434 Fragments arranged by size: 484 434 147 132 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 60 87 553 1335 Size: 60 27 466 782 Fragments arranged by size: 782 466 60 27 BseYI C'CCAG_C Cuts at: 0 676 1320 1335 Size: 676 644 15 Fragments arranged by size: 676 644 15 BsiHKAI G_wGCw'C Cuts at: 0 603 884 1172 1335 Size: 603 281 288 163 Fragments arranged by size: 603 288 281 163 BsmI GAATG_Cn' Cuts at: 0 397 1335 Size: 397 938 BsmBI CGTCTCn'nnnn_ Cuts at: 0 474 1335 Size: 474 861 Bsp1286I G_dGCh'C Cuts at: 0 70 603 884 1172 1329 1335 Size: 70 533 281 288 157 6 Fragments arranged by size: 533 288 281 157 70 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 188 464 1307 1335 Size: 188 276 843 28 Fragments arranged by size: 843 276 188 28 BsrBI CCG'CTC Cuts at: 0 705 1062 1335 Size: 705 357 273 Fragments arranged by size: 705 357 273 BsrGI T'GTAC_A Cuts at: 0 812 1335 Size: 812 523 BssSI C'ACGA_G Cuts at: 0 883 1335 Size: 883 452 BstAPI GCAn_nnn'nTGC Cuts at: 0 1009 1335 Size: 1009 326 BstXI CCAn_nnnn'nTGG Cuts at: 0 741 1335 Size: 741 594 BstYI r'GATC_y Cuts at: 0 752 953 1224 1335 Size: 752 201 271 111 Fragments arranged by size: 752 271 201 111 BtgI C'CryG_G Cuts at: 0 53 576 734 1335 Size: 53 523 158 601 Fragments arranged by size: 601 523 158 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 558 1335 Size: 558 777 BtsI GCAGTG_nn' Cuts at: 0 746 1335 Size: 746 589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1263 1298 1335 Size: 1263 35 37 Fragments arranged by size: 1263 37 35 DraI TTT'AAA Cuts at: 0 273 1335 Size: 273 1062 DraIII CAC_nnn'GTG Cuts at: 0 1108 1335 Size: 1108 227 DrdI GACnn_nn'nnGTC Cuts at: 0 295 387 514 1335 Size: 295 92 127 821 Fragments arranged by size: 821 295 127 92 EaeI y'GGCC_r Cuts at: 0 1178 1271 1335 Size: 1178 93 64 Fragments arranged by size: 1178 93 64 EarI CTCTTCn'nnn_ Cuts at: 0 169 190 530 761 1335 Size: 169 21 340 231 574 Fragments arranged by size: 574 340 231 169 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 101 136 706 1335 Size: 101 35 570 629 Fragments arranged by size: 629 570 101 35 EcoICRI GAG'CTC Cuts at: 0 882 1335 Size: 882 453 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 554 874 1335 Size: 554 320 461 Fragments arranged by size: 554 461 320 EcoNI CCTnn'n_nnAGG Cuts at: 0 523 1335 Size: 523 812 EcoO109I rG'GnC_Cy Cuts at: 0 1325 1335 Size: 1325 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 643 675 1295 1327 1335 Size: 643 32 620 32 8 Fragments arranged by size: 643 620 32 32 8 KpnI G_GTAC'C Cuts at: 0 1251 1335 Size: 1251 84 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 470 1335 Size: 470 865 MslI CAynn'nnrTG Cuts at: 0 739 1130 1335 Size: 739 391 205 Fragments arranged by size: 739 391 205 PasI CC'CwG_GG Cuts at: 0 14 63 1153 1335 Size: 14 49 1090 182 Fragments arranged by size: 1090 182 49 14 PflMI CCAn_nnn'nTGG Cuts at: 0 756 1335 Size: 756 579 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 484 516 601 633 1335 Size: 484 32 85 32 702 Fragments arranged by size: 702 484 85 32 32 PspOMI G'GGCC_C Cuts at: 0 1325 1335 Size: 1325 10 PstI C_TGCA'G Cuts at: 0 350 908 962 1005 1335 Size: 350 558 54 43 330 Fragments arranged by size: 558 350 330 54 43 SacI G_AGCT'C Cuts at: 0 884 1335 Size: 884 451 SapI GCTCTTCn'nnn_ Cuts at: 0 190 1335 Size: 190 1145 SexAI A'CCwGG_T Cuts at: 0 496 1335 Size: 496 839 SfcI C'TryA_G Cuts at: 0 346 904 958 1001 1335 Size: 346 558 54 43 334 Fragments arranged by size: 558 346 334 54 43 SmaI CCC'GGG Cuts at: 0 1330 1335 Size: 1330 5 SmlI C'TyrA_G Cuts at: 0 1171 1228 1335 Size: 1171 57 107 Fragments arranged by size: 1171 107 57 SrfI GCCC'GGGC Cuts at: 0 1330 1335 Size: 1330 5 StyI C'CwwG_G Cuts at: 0 330 1030 1335 Size: 330 700 305 Fragments arranged by size: 700 330 305 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 354 1088 1335 Size: 354 734 247 Fragments arranged by size: 734 354 247 TatI w'GTAC_w Cuts at: 0 263 710 812 1335 Size: 263 447 102 523 Fragments arranged by size: 523 447 263 102 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 741 1335 Size: 741 594 XmaI C'CCGG_G Cuts at: 0 1328 1335 Size: 1328 7 XmnI GAAnn'nnTTC Cuts at: 0 688 859 1335 Size: 688 171 476 Fragments arranged by size: 688 476 171 Enzymes that do cut: AarI Acc65I AcuI AflIII AleI AloI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI BglI BglII BlpI Bme1580I BmgBI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I KpnI MmeI MslI PasI PflMI PpiI PspOMI PstI SacI SapI SexAI SfcI SmaI SmlI SrfI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AlfI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BmrI BmtI BplI BpmI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BspEI BspHI BsrDI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I ClaI EagI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I XbaI XhoI ZraI