(Circular) (Six-base) MAPSORT of: pFN21AE6320.seq Check: 984 from: 1 to: 5908 pFN21AE6320 5908 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3718 278 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3251 1937 Size: 1314 4594 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 4035 4408 1563 Size: 2472 373 3063 Fragments arranged by size: 3063 2472 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2543 2957 3093 3971 5355 5623 1632 Size: 911 414 136 878 1384 268 1917 Fragments arranged by size: 1917 1384 911 878 414 268 136 AfeI AGC'GCT Cuts at: 2043 2366 2043 Size: 323 5585 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5711 AflIII A'CryG_T Cuts at: 1203 3033 4807 5784 1203 Size: 1830 1774 977 1327 Fragments arranged by size: 1830 1774 1327 977 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5908 AlwNI CAG_nnn'CTG Cuts at: 2380 3007 3651 5223 2380 Size: 627 644 1572 3065 Fragments arranged by size: 3065 1572 644 627 ApaLI G'TGCA_C Cuts at: 3965 5121 3965 Size: 1156 4752 ApoI r'AATT_y Cuts at: 1493 2879 3217 3443 3595 5704 1493 Size: 1386 338 226 152 2109 1697 Fragments arranged by size: 2109 1697 1386 338 226 152 AscI GG'CGCG_CC Cuts at: 2077 2077 Size: 5908 AseI AT'TA_AT Cuts at: 160 1032 4462 160 Size: 872 3430 1606 Fragments arranged by size: 3430 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2285 2315 3233 1870 Size: 73 192 150 30 918 4545 Fragments arranged by size: 4545 918 192 150 73 30 BamHI G'GATC_C Cuts at: 3238 3605 3238 Size: 367 5541 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2016 2582 3229 4684 618 Size: 325 217 627 229 566 647 1455 1842 Fragments arranged by size: 1842 1455 647 627 566 325 229 217 BanII G_rGCy'C Cuts at: 1391 2292 2445 3227 1391 Size: 901 153 782 4072 Fragments arranged by size: 4072 901 782 153 BbeI G_GCGC'C Cuts at: 2020 2020 Size: 5908 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4937 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4099 4133 1903 Size: 34 2162 34 3678 Fragments arranged by size: 3678 2162 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 2977 1548 Size: 1429 4479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4519 136 Size: 107 122 71 631 605 2847 1525 Fragments arranged by size: 2847 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5903 5903 Size: 5908 BlpI GC'TnA_GC Cuts at: 3325 3325 Size: 5908 Bme1580I G_kGCm'C Cuts at: 1790 3969 5125 1790 Size: 2179 1156 2573 Fragments arranged by size: 2573 2179 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2191 2846 3639 Size: 416 53 83 399 24 774 655 793 Cuts at: 3639 4597 442 Size: 958 1753 Fragments arranged by size: 1753 958 793 774 655 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2042 1056 Size: 986 4922 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2689 3089 3215 4568 1239 Size: 986 464 400 126 1353 2579 Fragments arranged by size: 2579 1353 986 464 400 126 Bpu10I CC'TnA_GC Cuts at: 3129 3129 Size: 5908 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 4033 4898 5196 2345 Size: 1688 865 298 3057 Fragments arranged by size: 3057 1688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3194 4571 882 Size: 617 1695 1377 2219 Fragments arranged by size: 2219 1695 1377 617 BsaAI yAC'GTr Cuts at: 493 1204 3036 3699 493 Size: 711 1832 663 2702 Fragments arranged by size: 2702 1832 711 663 BsaBI GATnn'nnATC Cuts at: 5902 5902 Size: 5908 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2017 3715 4097 4732 Size: 53 83 186 936 484 1698 382 635 Cuts at: 4732 275 Size: 1451 Fragments arranged by size: 1698 1451 936 635 484 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2869 4341 4739 5013 5160 5571 2869 Size: 1472 398 274 147 411 3206 Fragments arranged by size: 3206 1472 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3150 3180 1178 Size: 30 165 30 1747 30 3906 Fragments arranged by size: 3906 1747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3212 1186 Size: 2026 3882 BseYI C'CCAG_C Cuts at: 2400 2405 5111 5607 2400 Size: 5 2706 496 2701 Fragments arranged by size: 2706 2701 496 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2207 2976 1163 Size: 573 471 769 4095 Fragments arranged by size: 4095 769 573 471 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3358 4119 5147 1540 Size: 275 186 1357 761 1028 2301 Fragments arranged by size: 2301 1357 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 3057 3969 4054 5125 2181 Size: 876 912 85 1071 2964 Fragments arranged by size: 2964 1071 912 876 85 BsmI GAATG_Cn' Cuts at: 2825 2947 3419 3512 2825 Size: 122 472 93 5221 Fragments arranged by size: 5221 472 122 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2292 2445 3057 3227 3969 4054 Size: 399 391 111 153 612 170 742 85 Cuts at: 4054 5125 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 612 399 391 170 153 111 85 BspEI T'CCGG_A Cuts at: 5571 5571 Size: 5908 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3263 844 Size: 2419 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2134 3795 1394 Size: 429 311 1661 3507 Fragments arranged by size: 3507 1661 429 311 BsrDI GCAATG_nn' Cuts at: 65 1248 4403 4577 65 Size: 1183 3155 174 1396 Fragments arranged by size: 3155 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 2297 4552 4739 1700 Size: 110 338 149 2255 187 2869 Fragments arranged by size: 2869 2255 338 187 149 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5908 BssHII G'CGCG_C Cuts at: 1925 2061 2068 2077 2349 1925 Size: 136 7 9 272 5484 Fragments arranged by size: 5484 272 136 9 7 BssSI C'ACGA_G Cuts at: 3968 4980 3968 Size: 1012 4896 BstAPI GCAn_nnn'nTGC Cuts at: 794 2230 2380 794 Size: 1436 150 4322 Fragments arranged by size: 4322 1436 150 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstYI r'GATC_y Cuts at: 1972 3238 3605 3990 4007 5459 5545 5557 5903 Size: 1266 367 385 17 1452 86 12 346 Cuts at: 5903 1972 Size: 1977 Fragments arranged by size: 1977 1452 1266 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2114 513 Size: 552 939 24 86 4307 Fragments arranged by size: 4307 939 552 86 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2427 2482 487 Size: 683 56 1201 55 3913 Fragments arranged by size: 3913 1201 683 56 55 BtsI GCAGTG_nn' Cuts at: 2353 2649 3424 4218 4238 2353 Size: 296 775 794 20 4023 Fragments arranged by size: 4023 794 775 296 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4915 809 Size: 507 3599 1802 Fragments arranged by size: 3599 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873 Size: 54 1425 50 275 18 1525 889 1629 Cuts at: 5873 8 Size: 43 Fragments arranged by size: 1629 1525 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3355 1537 Size: 275 1543 4090 Fragments arranged by size: 4090 1543 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 2543 2832 3838 1353 Size: 980 210 289 1006 3423 Fragments arranged by size: 3423 1006 980 289 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2381 2438 4495 4869 5015 387 Size: 1064 138 792 57 2057 374 146 1280 Fragments arranged by size: 2057 1280 1064 792 374 146 138 57 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2543 2689 2957 3089 3093 3215 Size: 393 593 318 146 268 132 4 122 Cuts at: 3215 3971 4568 5355 5623 1239 Size: 756 597 787 268 1524 Fragments arranged by size: 1524 787 756 597 593 393 318 268 268 146 132 122 4 EcoO109I rG'GnC_Cy Cuts at: 2335 2377 3040 2335 Size: 42 663 5203 Fragments arranged by size: 5203 663 42 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 FspI TGC'GCA Cuts at: 4414 4414 Size: 5908 HaeII r_GCGC'y Cuts at: 1678 2020 2036 2045 2274 2368 2437 5055 1678 Size: 342 16 9 229 94 69 2618 2531 Fragments arranged by size: 2618 2531 342 229 94 69 16 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4549 4581 4623 4655 1373 Size: 32 135 32 2977 32 42 32 2626 Fragments arranged by size: 2977 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3252 3498 669 Size: 988 281 1314 246 3079 Fragments arranged by size: 3079 1314 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KasI G'GCGC_C Cuts at: 2016 2016 Size: 5908 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5022 5206 1305 Size: 3717 184 2007 Fragments arranged by size: 3717 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2532 2687 3866 4225 4384 518 Size: 648 1366 155 1179 359 159 2042 Fragments arranged by size: 2042 1366 1179 648 359 159 155 MspA1I CmG'CkG Cuts at: 1606 2030 2172 2504 3324 4001 5149 5394 1606 Size: 424 142 332 820 677 1148 245 2120 Fragments arranged by size: 2120 1148 820 677 424 332 245 142 NaeI GCC'GGC Cuts at: 2299 2299 Size: 5908 NarI GG'CG_CC Cuts at: 2017 2017 Size: 5908 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4417 Fragments arranged by size: 4417 939 552 NdeI CA'TA_TG Cuts at: 387 2620 387 Size: 2233 3675 NgoMIV G'CCGG_C Cuts at: 2297 2297 Size: 5908 NheI G'CTAG_C Cuts at: 1052 2038 1052 Size: 986 4922 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 3266 4811 1132 Size: 97 2037 1545 2229 Fragments arranged by size: 2229 2037 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5908 PciI A'CATG_T Cuts at: 4807 4807 Size: 5908 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5908 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PmlI CAC'GTG Cuts at: 3036 3036 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4009 5490 5522 3977 Size: 32 1481 32 4363 Fragments arranged by size: 4363 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 2568 2922 3478 2568 Size: 354 556 4998 Fragments arranged by size: 4998 556 354 PspXI vC'TCGA_Gb Cuts at: 2135 2135 Size: 5908 PstI C_TGCA'G Cuts at: 830 2270 2460 3260 830 Size: 1440 190 800 3478 Fragments arranged by size: 3478 1440 800 190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 SacII CC_GC'GG Cuts at: 2031 2031 Size: 5908 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 4156 5868 4156 Size: 1712 4196 SexAI A'CCwGG_T Cuts at: 3006 3006 Size: 5908 SfcI C'TryA_G Cuts at: 826 1045 2095 2266 2456 3256 4391 5072 5263 Size: 219 1050 171 190 800 1135 681 191 Cuts at: 5263 826 Size: 1471 Fragments arranged by size: 1471 1135 1050 800 681 219 191 190 171 SfoI GGC'GCC Cuts at: 2018 2018 Size: 5908 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5908 SmaI CCC'GGG Cuts at: 1872 3235 1872 Size: 1363 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2135 2360 4012 4913 5175 820 Size: 197 926 192 225 1652 901 262 1553 Fragments arranged by size: 1652 1553 926 901 262 225 197 192 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 3266 1132 Size: 2134 3774 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 2249 1517 Size: 732 5176 StyI C'CwwG_G Cuts at: 513 1065 2004 3347 513 Size: 552 939 1343 3074 Fragments arranged by size: 3074 1343 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3935 3952 4105 4290 4735 325 Size: 1255 2355 17 153 185 445 1498 Fragments arranged by size: 2355 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 4154 5866 Size: 274 80 33 51 1443 189 1988 1712 Cuts at: 5866 96 Size: 138 Fragments arranged by size: 1988 1712 1443 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3150 3182 5868 5900 3150 Size: 32 2686 32 3158 Fragments arranged by size: 3158 2686 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2990 1316 Size: 1674 4234 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2686 2686 Size: 5908 XhoI C'TCGA_G Cuts at: 1943 2135 1943 Size: 192 5716 XmaI C'CCGG_G Cuts at: 1870 3233 1870 Size: 1363 4545 XmnI GAAnn'nnTTC Cuts at: 3078 4037 3078 Size: 959 4949 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3716 276 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PsrI PvuII RsrII SacI SanDI SapI SfiI SrfI SwaI