(Circular) (Six-base) MAPSORT of: pFN21AE1125.seq Check: 7673 from: 1 to: 6421 pFN21AE1125 6421 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3245 3350 3245 Size: 105 6316 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4231 278 Size: 53 83 186 936 2695 2468 Fragments arranged by size: 2695 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3764 1937 Size: 1827 4594 Acc65I G'GTAC_C Cuts at: 3742 3742 Size: 6421 AclI AA'CG_TT Cuts at: 1563 4548 4921 1563 Size: 2985 373 3063 Fragments arranged by size: 3063 2985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3083 4484 5868 6136 1632 Size: 1451 1401 1384 268 1917 Fragments arranged by size: 1917 1451 1401 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6224 AflIII A'CryG_T Cuts at: 1203 5320 6297 1203 Size: 4117 977 1327 Fragments arranged by size: 4117 1327 977 AgeI A'CCGG_T Cuts at: 5252 5252 Size: 6421 AhdI GACnn_n'nnGTC Cuts at: 2455 5150 2455 Size: 2695 3726 AleI CACnn'nnGTG Cuts at: 1166 3246 3413 1166 Size: 2080 167 4174 Fragments arranged by size: 4174 2080 167 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6421 AlwNI CAG_nnn'CTG Cuts at: 2629 4164 5736 2629 Size: 1535 1572 3314 Fragments arranged by size: 3314 1572 1535 ApaI G_GGCC'C Cuts at: 2161 2769 3046 3054 2161 Size: 608 277 8 5528 Fragments arranged by size: 5528 608 277 8 ApaLI G'TGCA_C Cuts at: 4478 5634 4478 Size: 1156 5265 ApoI r'AATT_y Cuts at: 1493 3730 3956 4108 6217 1493 Size: 2237 226 152 2109 1697 Fragments arranged by size: 2237 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4975 160 Size: 872 3943 1606 Fragments arranged by size: 3943 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 AvaI C'yCGr_G Cuts at: 1870 1943 2126 2153 2502 2760 2973 3180 3508 Size: 73 183 27 349 258 213 207 328 Cuts at: 3508 3746 1870 Size: 238 4545 Fragments arranged by size: 4545 349 328 258 238 213 207 183 73 27 BamHI G'GATC_C Cuts at: 3751 4118 3751 Size: 367 6054 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2149 2276 2895 3292 3440 Size: 325 217 627 362 127 619 397 148 Cuts at: 3440 3742 5197 618 Size: 302 1455 1842 Fragments arranged by size: 1842 1455 627 619 397 362 325 302 217 148 127 BanII G_rGCy'C Cuts at: 1391 2161 2769 2857 3046 3054 3354 3534 3715 Size: 770 608 88 189 8 300 180 181 Cuts at: 3715 3740 1391 Size: 25 4072 Fragments arranged by size: 4072 770 608 300 189 181 180 88 25 8 BbeI G_GCGC'C Cuts at: 3296 3296 Size: 6421 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2217 928 Size: 971 318 5132 Fragments arranged by size: 5132 971 318 BbvCI CC'TCA_GC Cuts at: 3371 3371 Size: 6421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4612 4646 1903 Size: 34 2675 34 3678 Fragments arranged by size: 3678 2675 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4313 5523 4313 Size: 1210 5211 BclI T'GATC_A Cuts at: 1548 1548 Size: 6421 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2184 2764 Size: 107 122 71 631 605 337 175 580 Cuts at: 2764 5032 136 Size: 2268 1525 Fragments arranged by size: 2268 1525 631 605 580 337 175 122 107 71 BglII A'GATC_T Cuts at: 2340 6416 2340 Size: 4076 2345 BlpI GC'TnA_GC Cuts at: 3838 3838 Size: 6421 Bme1580I G_kGCm'C Cuts at: 1790 2161 2526 2585 2769 2898 3003 3046 3054 Size: 371 365 59 184 129 105 43 8 Cuts at: 3054 3443 4482 5638 1790 Size: 389 1039 1156 2573 Fragments arranged by size: 2573 1156 1039 389 371 365 184 129 105 59 43 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6421 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4152 5110 442 Size: 416 53 83 399 24 2735 958 1753 Fragments arranged by size: 2735 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6421 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2467 3460 2467 Size: 993 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2772 2790 2871 3101 3245 3305 3347 Size: 1112 421 18 81 230 144 60 42 Cuts at: 3347 3455 5081 1239 Size: 108 1626 2579 Fragments arranged by size: 2579 1626 1112 421 230 144 108 81 60 42 18 Bpu10I CC'TnA_GC Cuts at: 3371 3543 3371 Size: 172 6249 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4546 5411 5709 4546 Size: 865 298 5258 Fragments arranged by size: 5258 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2050 5084 882 Size: 617 551 3034 2219 Fragments arranged by size: 3034 2219 617 551 BsaAI yAC'GTr Cuts at: 493 1204 3682 4212 493 Size: 711 2478 530 2702 Fragments arranged by size: 2702 2478 711 530 BsaBI GATnn'nnATC Cuts at: 6415 6415 Size: 6421 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3293 4228 4610 5245 Size: 53 83 186 936 1760 935 382 635 Cuts at: 5245 275 Size: 1451 Fragments arranged by size: 1760 1451 936 935 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2365 4854 5252 5526 5673 6084 2365 Size: 2489 398 274 147 411 2702 Fragments arranged by size: 2702 2489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6196 Fragments arranged by size: 6196 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2351 2352 2384 2387 3089 1186 Size: 1162 3 1 32 3 702 4518 Fragments arranged by size: 4518 1162 702 32 3 3 1 BseYI C'CCAG_C Cuts at: 2160 2240 2622 2682 2688 2730 2754 2770 2939 Size: 80 382 60 6 42 24 16 169 Cuts at: 2939 2955 3093 5624 6120 2160 Size: 16 138 2531 496 2461 Fragments arranged by size: 2531 2461 496 382 169 138 80 60 42 24 16 16 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2999 1163 Size: 573 1263 4585 Fragments arranged by size: 4585 1263 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2304 3160 3871 4632 5660 1540 Size: 275 186 303 856 711 761 1028 2301 Fragments arranged by size: 2301 1028 856 761 711 303 275 186 BsiHKAI G_wGCw'C Cuts at: 2269 3518 3687 4482 4567 5638 2269 Size: 1249 169 795 85 1071 3052 Fragments arranged by size: 3052 1249 1071 795 169 85 BsmI GAATG_Cn' Cuts at: 3932 4025 3932 Size: 93 6328 BsmBI CGTCTCn'nnnn_ Cuts at: 2028 2028 Size: 6421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 2269 2526 2585 2769 2857 2898 Size: 399 371 108 257 59 184 88 41 Cuts at: 2898 3003 3046 3054 3354 3443 3518 3534 3687 Size: 105 43 8 300 89 75 16 153 Cuts at: 3687 3715 3740 4482 4567 5638 1391 Size: 28 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 371 300 257 184 153 108 105 89 88 85 75 59 43 41 28 25 16 8 BspEI T'CCGG_A Cuts at: 2365 6084 2365 Size: 3719 2702 BspHI T'CATG_A Cuts at: 4310 6040 4310 Size: 1730 4691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3245 3350 3776 844 Size: 2401 105 426 3489 Fragments arranged by size: 3489 2401 426 105 BsrBI CCG'CTC Cuts at: 1394 1823 4308 1394 Size: 429 2485 3507 Fragments arranged by size: 3507 2485 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4916 5090 65 Size: 1183 3668 174 1396 Fragments arranged by size: 3668 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3007 3468 5065 5252 1700 Size: 110 1197 461 1597 187 2869 Fragments arranged by size: 2869 1597 1197 461 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6421 BssHII G'CGCG_C Cuts at: 1925 2164 1925 Size: 239 6182 BssSI C'ACGA_G Cuts at: 4481 5493 4481 Size: 1012 5409 BstAPI GCAn_nnn'nTGC Cuts at: 794 2264 2998 3103 794 Size: 1470 734 105 4112 Fragments arranged by size: 4112 1470 734 105 BstBI TT'CG_AA Cuts at: 5225 5225 Size: 6421 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6421 BstYI r'GATC_y Cuts at: 1972 2340 2864 3751 4118 4503 4520 5972 6058 Size: 368 524 887 367 385 17 1452 86 Cuts at: 6058 6070 6416 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 887 524 385 368 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2385 2700 2385 Size: 315 6106 BtgI C'CryG_G Cuts at: 513 1065 2004 3378 513 Size: 552 939 1374 3556 Fragments arranged by size: 3556 1374 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3208 487 Size: 683 56 1982 3700 Fragments arranged by size: 3700 1982 683 56 BtsI GCAGTG_nn' Cuts at: 2462 3400 3702 3937 4731 4751 2462 Size: 938 302 235 794 20 4132 Fragments arranged by size: 4132 938 794 302 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6386 DraI TTT'AAA Cuts at: 3725 3725 Size: 6421 DraIII CAC_nnn'GTG Cuts at: 2998 2998 Size: 6421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5428 809 Size: 507 4112 1802 Fragments arranged by size: 4112 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2535 3157 3550 Size: 54 1425 50 275 18 705 622 393 Cuts at: 3550 3868 4757 6386 8 Size: 318 889 1629 43 Fragments arranged by size: 1629 1425 889 705 622 393 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 3868 1537 Size: 275 1345 711 4090 Fragments arranged by size: 4090 1345 711 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4351 1353 Size: 2998 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5008 5382 5528 387 Size: 1064 138 3419 374 146 1280 Fragments arranged by size: 3419 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 2772 2790 2871 3083 3101 3245 Size: 393 719 421 18 81 212 18 144 Cuts at: 3245 3305 3347 3455 4484 5081 5868 6136 1239 Size: 60 42 108 1029 597 787 268 1524 Fragments arranged by size: 1524 1029 787 719 597 421 393 268 212 144 108 81 60 42 18 18 EcoO109I rG'GnC_Cy Cuts at: 2157 2381 2389 2765 2766 3042 3043 3050 3051 Size: 224 8 376 1 276 1 7 1 Cuts at: 3051 2157 Size: 5527 Fragments arranged by size: 5527 376 276 224 8 7 1 1 1 EcoRI G'AATT_C Cuts at: 3730 4108 3730 Size: 378 6043 FspI TGC'GCA Cuts at: 3252 4927 3252 Size: 1675 4746 HaeII r_GCGC'y Cuts at: 1678 3296 5568 1678 Size: 1618 2272 2531 Fragments arranged by size: 2531 2272 1618 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2437 2441 2469 2473 5062 Size: 32 135 32 865 4 28 4 2589 Cuts at: 5062 5094 5136 5168 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2589 865 135 42 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 3145 3765 4011 669 Size: 988 281 1207 620 246 3079 Fragments arranged by size: 3079 1207 988 620 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6421 HpaI GTT'AAC Cuts at: 4011 4011 Size: 6421 KasI G'GCGC_C Cuts at: 3292 3292 Size: 6421 KpnI G_GTAC'C Cuts at: 3746 3746 Size: 6421 MfeI C'AATT_G Cuts at: 4020 4020 Size: 6421 MluI A'CGCG_T Cuts at: 6297 6297 Size: 6421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2731 3671 5535 5719 1305 Size: 1426 940 1864 184 2007 Fragments arranged by size: 2007 1864 1426 940 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2537 10 Size: 54 1425 343 705 3894 Fragments arranged by size: 3894 1425 705 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3246 3413 4379 4738 4897 518 Size: 648 2080 167 966 359 159 2042 Fragments arranged by size: 2080 2042 966 648 359 167 159 MspA1I CmG'CkG Cuts at: 1606 3088 3214 3837 4514 5662 5907 1606 Size: 1482 126 623 677 1148 245 2120 Fragments arranged by size: 2120 1482 1148 677 623 245 126 NarI GG'CG_CC Cuts at: 3293 3293 Size: 6421 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4930 Fragments arranged by size: 4930 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6421 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6421 NotI GC'GGCC_GC Cuts at: 3868 3868 Size: 6421 NspI r_CATG'y Cuts at: 1132 1229 3370 3779 5324 1132 Size: 97 2141 409 1545 2229 Fragments arranged by size: 2229 2141 1545 409 97 PasI CC'CwG_GG Cuts at: 1348 2415 3038 3047 1348 Size: 1067 623 9 4722 Fragments arranged by size: 4722 1067 623 9 PciI A'CATG_T Cuts at: 5320 5320 Size: 6421 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6421 PfoI T'CCnGG_A Cuts at: 2848 2848 Size: 6421 PmeI GTTT'AAAC Cuts at: 3725 3725 Size: 6421 PmlI CAC'GTG Cuts at: 3682 3682 Size: 6421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4490 4522 6003 6035 4490 Size: 32 1481 32 4876 Fragments arranged by size: 4876 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6421 PsiI TTA'TAA Cuts at: 3991 3991 Size: 6421 PspOMI G'GGCC_C Cuts at: 2157 2765 3042 3050 2157 Size: 608 277 8 5528 Fragments arranged by size: 5528 608 277 8 PstI C_TGCA'G Cuts at: 830 2575 3773 830 Size: 1745 1198 3478 Fragments arranged by size: 3478 1745 1198 PvuI CG_AT'CG Cuts at: 2001 2304 2001 Size: 303 6118 PvuII CAG'CTG Cuts at: 3088 3214 3088 Size: 126 6295 SalI G'TCGA_C Cuts at: 1936 3763 1936 Size: 1827 4594 SbfI CC_TGCA'GG Cuts at: 3773 3773 Size: 6421 ScaI AGT'ACT Cuts at: 4669 6381 4669 Size: 1712 4709 SexAI A'CCwGG_T Cuts at: 3024 3024 Size: 6421 SfcI C'TryA_G Cuts at: 826 1045 2571 3277 3769 4904 5585 5776 826 Size: 219 1526 706 492 1135 681 191 1471 Fragments arranged by size: 1526 1471 1135 706 681 492 219 191 SfoI GGC'GCC Cuts at: 3294 3294 Size: 6421 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6421 SmaI CCC'GGG Cuts at: 1872 2128 2155 2504 3182 3748 1872 Size: 256 27 349 678 566 4545 Fragments arranged by size: 4545 678 566 349 256 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 4525 5426 5688 820 Size: 197 926 2582 901 262 1553 Fragments arranged by size: 2582 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4212 493 Size: 3719 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6421 SphI G_CATG'C Cuts at: 1132 3370 3779 1132 Size: 2238 409 3774 Fragments arranged by size: 3774 2238 409 SrfI GCCC'GGGC Cuts at: 3182 3182 Size: 6421 SspI AAT'ATT Cuts at: 5 52 4345 5 Size: 47 4293 2081 Fragments arranged by size: 4293 2081 47 StuI AGG'CCT Cuts at: 1517 3389 1517 Size: 1872 4549 StyI C'CwwG_G Cuts at: 513 1065 2004 2600 3860 513 Size: 552 939 596 1260 3074 Fragments arranged by size: 3074 1260 939 596 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2032 2768 4448 4465 4618 4803 5248 Size: 1255 452 736 1680 17 153 185 445 Cuts at: 5248 325 Size: 1498 Fragments arranged by size: 1680 1498 1255 736 452 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4667 6379 96 Size: 274 80 33 51 1443 2690 1712 138 Fragments arranged by size: 2690 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6381 6413 6381 Size: 32 6389 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6421 XbaI T'CTAG_A Cuts at: 3757 3757 Size: 6421 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6421 XmaI C'CCGG_G Cuts at: 1870 2126 2153 2502 3180 3746 1870 Size: 256 27 349 678 566 4545 Fragments arranged by size: 4545 678 566 349 256 27 XmnI GAAnn'nnTTC Cuts at: 2078 2117 2214 2313 4550 2078 Size: 39 97 99 2237 3949 Fragments arranged by size: 3949 2237 99 97 39 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4229 276 Size: 53 83 186 936 2695 2468 Fragments arranged by size: 2695 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SwaI XcmI