##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HphI        KasI        KpnI        Ksp632I     
KspI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MscI        
MslI        MspA1I      MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tsp45I      Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccIII         41  TCCGGA               T^CCGGA
AciI           17  CCGC                 CCGC(-3/-1)
AfaI           48  GTAC                 GT^AC
AocI          286  CCTCAGG              CC^T[ACGT]AGG
BfaI          199  CTAG                 C^TAG
BpmI          167  CTCCAG               CTGGAG(16/14)
BsaJI          83  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI          41  TCCGGA               [AT]^CCGG[AT]
BseAI          41  TCCGGA               T^CCGGA
BseRI         292  GAGGAG               GAGGAG(10/8)
BsiYI         198  CCTAGAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          198  CCTAGAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI          41  TCCGGA               T^CCGGA
BsrI          204  ACTGG                ACTGG(1/-1)
BstAPI        262  GCAAAGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI          83  CCAAGGTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        286  CCTCAGG              CC^T[ACGT]AGG
Cfr13I          3  GGCCC                G^G[ACGT]CC
DraI          141  TTTAAA               TTT^AAA
EaeI          206  TGGCCG               [CT]^GGCC[GA]
Eco81I        286  CCTCAGG              CC^T[ACGT]AGG
EcoT14I        83  CCAAGG               C^C[AT][AT]GG
HapII          42  CCGG                 C^CGG
HindIII       265  AAGCTT               A^AGCTT
HinfI          22  GACTC                G^A[ACGT]TC
HpaII          42  CCGG                 C^CGG
MaeI          199  CTAG                 C^TAG
MroI           41  TCCGGA               T^CCGGA
MseI          142  TTAA                 T^TAA
MspI           42  CCGG                 C^CGG
PleI           22  GACTC                GAGTC(4/5)
RsaI           48  GTAC                 GT^AC
Sau96I          3  GGCCC                G^G[ACGT]CC
SfaNI         147  GCATC                GCATC(5/9)
StyI           83  CCAAGG               C^C[AT][AT]GG
TaqI           25  TCGA                 T^CGA
Tru9I         142  TTAA                 T^TAA
TspRI         126  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        25  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BbvI          160  GCTGC                GCAGC(8/12)
              189  GCTGC               
Cac8I         267  GCTTGC               GC[ACGT]^[ACGT]GC
              300  GCGTGC              
HaeIII          3  GGCC                 GG^CC
              207  GGCC                
MboII         106  GAAGA                GAAGA(8/7)
              241  GAAGA               
MwoI           69  GCTGGACACGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              262  GCAAAGCTTGC         
TseI          160  GCTGC                G^C[AT]GC
              189  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AluI        DdeI        Fnu4HI      ItaI        MnlI