(Circular) (Six-base) MAPSORT of: pFN21AB8711.seq Check: 405 from: 1 to: 6151 pFN21AB8711 6151 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 1937 Size: 1557 4594 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2979 3117 4214 5598 5866 1632 Size: 1347 138 1097 1384 268 1917 Fragments arranged by size: 1917 1384 1347 1097 268 138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 2143 2526 5050 6027 1203 Size: 940 383 2524 977 1327 Fragments arranged by size: 2524 1327 977 940 383 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 3894 5466 3894 Size: 1572 4579 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 2288 2789 3460 3686 3838 5947 1493 Size: 795 501 671 226 152 2109 1697 Fragments arranged by size: 2109 1697 795 671 501 226 152 AseI AT'TA_AT Cuts at: 160 1032 2906 4705 160 Size: 872 1874 1799 1606 Fragments arranged by size: 1874 1799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 3476 1870 Size: 73 1533 4545 Fragments arranged by size: 4545 1533 73 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3472 4927 618 Size: 325 217 627 1685 1455 1842 Fragments arranged by size: 1842 1685 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3470 1391 Size: 2079 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4342 4376 1903 Size: 34 2405 34 3678 Fragments arranged by size: 3678 2405 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 1548 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4762 136 Size: 107 122 71 631 605 3090 1525 Fragments arranged by size: 3090 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3135 6146 3135 Size: 3011 3140 BlpI GC'TnA_GC Cuts at: 3568 3568 Size: 6151 Bme1580I G_kGCm'C Cuts at: 1790 3225 4212 5368 1790 Size: 1435 987 1156 2573 Fragments arranged by size: 2573 1435 1156 987 BmgBI CAC'GTC Cuts at: 1318 2527 1318 Size: 1209 4942 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2291 3882 4840 Size: 416 53 83 399 24 874 1591 958 Cuts at: 4840 442 Size: 1753 Fragments arranged by size: 1753 1591 958 874 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2696 1056 Size: 1640 4511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4811 1239 Size: 3572 2579 Bpu10I CC'TnA_GC Cuts at: 3016 3268 3339 3016 Size: 252 71 5828 Fragments arranged by size: 5828 252 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2037 2250 4276 5141 5439 2037 Size: 213 2026 865 298 2749 Fragments arranged by size: 2749 2026 865 298 213 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4814 882 Size: 617 3315 2219 Fragments arranged by size: 3315 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 6145 6145 Size: 6151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3958 4340 4975 275 Size: 53 83 186 936 2425 382 635 1451 Fragments arranged by size: 2425 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4584 4982 5256 5403 5814 4584 Size: 398 274 147 411 4921 Fragments arranged by size: 4921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5926 Fragments arranged by size: 5926 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2517 1186 Size: 1331 4820 BseYI C'CCAG_C Cuts at: 2373 5354 5850 2373 Size: 2981 496 2674 Fragments arranged by size: 2981 2674 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2506 2909 1163 Size: 573 770 403 4405 Fragments arranged by size: 4405 770 573 403 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 4362 5390 1540 Size: 275 186 1600 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2509 4212 4297 5368 2509 Size: 1703 85 1071 3292 Fragments arranged by size: 3292 1703 1071 85 BsmI GAATG_Cn' Cuts at: 3662 3755 3662 Size: 93 6058 BsmBI CGTCTCn'nnnn_ Cuts at: 2090 3192 2090 Size: 1102 5049 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2509 3225 3470 4212 4297 5368 1391 Size: 399 719 716 245 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 719 716 399 245 85 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 4040 5770 4040 Size: 1730 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3126 3343 3506 844 Size: 2282 217 163 3489 Fragments arranged by size: 3489 2282 217 163 BsrBI CCG'CTC Cuts at: 1394 1823 4038 1394 Size: 429 2215 3507 Fragments arranged by size: 3507 2215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4646 4820 65 Size: 1183 3398 174 1396 Fragments arranged by size: 3398 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4795 4982 1700 Size: 110 2985 187 2869 Fragments arranged by size: 2985 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6151 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6151 BstYI r'GATC_y Cuts at: 1972 2650 3135 3481 3848 4233 4250 5702 5788 Size: 678 485 346 367 385 17 1452 86 Cuts at: 5788 5800 6146 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 678 485 385 367 346 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5599 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 3667 4461 4481 3667 Size: 794 20 5337 Fragments arranged by size: 5337 794 20 ClaI AT'CG_AT Cuts at: 1554 2044 1554 Size: 490 5661 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 2802 3455 2802 Size: 653 5498 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 809 Size: 507 3842 1802 Fragments arranged by size: 3842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3202 3598 4487 Size: 54 1425 50 275 18 1372 396 889 Cuts at: 4487 6116 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1372 889 396 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2838 3153 3380 4081 1353 Size: 1485 315 227 701 3423 Fragments arranged by size: 3423 1485 701 315 227 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4738 5112 5258 387 Size: 1064 138 3149 374 146 1280 Fragments arranged by size: 3149 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2979 3117 4214 4811 5598 5866 1239 Size: 393 1347 138 1097 597 787 268 1524 Fragments arranged by size: 1524 1347 1097 787 597 393 268 138 EcoO109I rG'GnC_Cy Cuts at: 2356 3020 3084 2356 Size: 664 64 5423 Fragments arranged by size: 5423 664 64 EcoRI G'AATT_C Cuts at: 3460 3838 3460 Size: 378 5773 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2125 2615 2669 2125 Size: 490 54 5607 Fragments arranged by size: 5607 490 54 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 5298 1678 Size: 3620 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2194 2226 2661 2693 2981 Size: 32 135 32 622 32 435 32 288 Cuts at: 2981 3013 3032 3064 3380 3412 4792 4824 4866 Size: 32 19 32 316 32 1380 32 42 Cuts at: 4866 4898 1373 Size: 32 2626 Fragments arranged by size: 2626 1380 622 435 316 288 135 42 32 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 3495 3741 669 Size: 988 281 1557 246 3079 Fragments arranged by size: 3079 1557 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6151 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 2229 3750 2229 Size: 1521 4630 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5265 5449 1305 Size: 3960 184 2007 Fragments arranged by size: 3960 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4329 Fragments arranged by size: 4329 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2512 4109 4468 4627 518 Size: 648 1346 1597 359 159 2042 Fragments arranged by size: 2042 1597 1346 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3188 3567 4244 5392 5637 1606 Size: 1582 379 677 1148 245 2120 Fragments arranged by size: 2120 1582 1148 677 379 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5599 NdeI CA'TA_TG Cuts at: 387 2785 387 Size: 2398 3753 NheI G'CTAG_C Cuts at: 1052 2692 1052 Size: 1640 4511 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2147 2692 3015 3509 5054 1132 Size: 97 918 545 323 494 1545 2229 Fragments arranged by size: 2229 1545 918 545 494 323 97 PasI CC'CwG_GG Cuts at: 1348 2028 3235 1348 Size: 680 1207 4264 Fragments arranged by size: 4264 1207 680 PciI A'CATG_T Cuts at: 2143 5050 2143 Size: 2907 3244 PflMI CCAn_nnn'nTGG Cuts at: 1108 2422 1108 Size: 1314 4837 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3152 3184 4220 4252 5733 5765 3152 Size: 32 1036 32 1481 32 3538 Fragments arranged by size: 3538 1481 1036 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3020 3084 3020 Size: 64 6087 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3721 3721 Size: 6151 PstI C_TGCA'G Cuts at: 830 3503 830 Size: 2673 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SanDI GG'GwC_CC Cuts at: 3084 3084 Size: 6151 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SfcI C'TryA_G Cuts at: 826 1045 3499 4634 5315 5506 826 Size: 219 2454 1135 681 191 1471 Fragments arranged by size: 2454 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2052 2265 4255 5156 5418 820 Size: 197 926 109 213 1990 901 262 1553 Fragments arranged by size: 1990 1553 926 901 262 213 197 109 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3015 3509 1132 Size: 1883 494 3774 Fragments arranged by size: 3774 1883 494 SspI AAT'ATT Cuts at: 5 52 2090 4075 5 Size: 47 2038 1985 2081 Fragments arranged by size: 2081 2038 1985 47 StuI AGG'CCT Cuts at: 1517 2108 3320 1517 Size: 591 1212 4348 Fragments arranged by size: 4348 1212 591 StyI C'CwwG_G Cuts at: 513 1065 2302 3153 3252 3590 513 Size: 552 1237 851 99 338 3074 Fragments arranged by size: 3074 1237 851 552 338 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4178 4195 4348 4533 4978 325 Size: 1255 2598 17 153 185 445 1498 Fragments arranged by size: 2598 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2233 2873 4397 Size: 274 80 33 51 1443 256 640 1524 Cuts at: 4397 6109 96 Size: 1712 138 Fragments arranged by size: 1712 1524 1443 640 274 256 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6111 6143 6111 Size: 32 6119 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6151 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3232 3232 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 4280 4280 Size: 6151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI