Restriction map for
pFN21AE1410
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeIII MamI
MboI MfeI MflI MluI MroI
MseI MslI MspA1I MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tru9I
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 108 CGCG CG^CG
AciI 109 GCGG CCGC(-3/-1)
AspHI 54 GAGCTC G[AT]GC[AT]^C
BalI 113 TGGCCA TGG^CCA
BanII 54 GAGCTC G[GA]GC[CT]^C
BbsI 13 GAAGAC GAAGAC(2/6)
BcnI 50 CCGGG CC^[GC]GG
BmyI 54 GAGCTC G[AGT]GC[ACT]^C
BpuAI 13 GAAGAC GAAGAC(2/6)
BsaAI 144 TACGTG [CT]AC^GT[GA]
BsaJI 35 CCCTGG C^C[ACGT][ACGT]GG
BsiHKAI 54 GAGCTC G[AT]GC[AT]^C
Bsp1286I 54 GAGCTC G[AGT]GC[ACT]^C
BspMI 28 ACCTGC ACCTGC(4/8)
BsrI 314 ACTGG ACTGG(1/-1)
BstF5I 174 GGATG GGATG(2/0)
BstUI 108 CGCG CG^CG
Cac8I 305 GCCAGC GC[ACGT]^[ACGT]GC
DrdI 313 GACTGGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 174 GGATG GGATG(9/13)
HapII 50 CCGG C^CGG
HinfI 329 GAATC G^A[ACGT]TC
HpaII 50 CCGG C^CGG
MboII 13 GAAGA GAAGA(8/7)
MluNI 113 TGGCCA TGG^CCA
MscI 113 TGGCCA TGG^CCA
MspI 50 CCGG C^CGG
MvnI 108 CGCG CG^CG
NciI 50 CCGGG CC^[GC]GG
NspI 263 GCATGT [GA]CATG^[CT]
PflMI 165 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 156 CTGCAG CTGCA^G
SacI 54 GAGCTC GAGCT^C
SfaNI 175 GATGC GCATC(5/9)
SfcI 156 CTGCAG C^T[GA][CT]AG
SmlI 94 CTCAAG C^T[CT][GA]AG
TfiI 329 GAATC G^A[AT]TC
Van91I 165 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 162 GGTCC G^G[AT]CC
321 GGTCC
BbvI 260 GCTGC GCAGC(8/12)
293 GCTGC
BsiYI 152 CCATCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
165 CCAGGTGATGG
BslI 152 CCATCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
165 CCAGGTGATGG
BsmFI 128 GGGAC GGGAC(10/14)
267 GTCCC
BstNI 36 CCTGG CC^[AT]GG
165 CCAGG
CfoI 32 GCGC GCG^C
149 GCGC
Cfr13I 162 GGTCC G^G[ACGT]CC
321 GGTCC
DdeI 57 CTCAG C^T[ACGT]AG
184 CTTAG
EaeI 2 TGGCCG [CT]^GGCC[GA]
113 TGGCCA
EcoRII 36 CCTGG ^CC[AT]GG
165 CCAGG
Fnu4HI 260 GCTGC GC^[ACGT]GC
293 GCTGC
HhaI 32 GCGC GCG^C
149 GCGC
HinP1I 32 GCGC G^CGC
149 GCGC
HphI 168 GGTGA GGTGA(8/7)
276 GGTGA
ItaI 260 GCTGC GC^[ACGT]GC
293 GCTGC
MvaI 36 CCTGG CC^[AT]GG
165 CCAGG
NlaIII 117 CATG CATG^
264 CATG
NlaIV 61 GGCTCC GG[ACGT]^[ACGT]CC
161 GGGTCC
Sau96I 162 GGTCC G^G[ACGT]CC
321 GGTCC
TseI 260 GCTGC G^C[AT]GC
293 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI BssKI HaeIII MaeII MnlI
MwoI ScrFI