################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BsrI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 407 GGTACC G^GTACC AfaI 408 GTAC GT^AC AlwI 308 GGATC GGATC(4/5) AocI 672 CCTGAGG CC^T[ACGT]AGG Asp718I 407 GGTACC G^GTACC AvaI 39 CTCGGG C^[CT]CG[GA]G BbsI 900 GTCTTC GAAGAC(2/6) BpuAI 900 GTCTTC GAAGAC(2/6) BsaAI 151 TACGTG [CT]AC^GT[GA] BsaI 766 GAGACC GGTCTC(1/5) BsaWI 254 ACCGGA [AT]^CCGG[AT] BseRI 996 GAGGAG GAGGAG(10/8) BsgI 853 GTGCAG GTGCAG(16/14) BsiEI 117 CGGCCG CG[GA][CT]^CG BsoBI 39 CTCGGG C^[CT]CG[GA]G BsrDI 933 CATTGC GCAATG(2/0) BsrFI 557 GCCGGT [GA]^CCGG[CT] BssSI 760 CTCGTG CACGAG(-5/-1) BstAPI 165 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 672 CCTGAGG CC^T[ACGT]AGG Cfr10I 557 GCCGGT [GA]^CCGG[CT] EagI 117 CGGCCG C^GGCCG EarI 1002 GAAGAG CTCTTC(1/4) EclXI 117 CGGCCG C^GGCCG Eco52I 117 CGGCCG C^GGCCG Eco81I 672 CCTGAGG CC^T[ACGT]AGG EcoNI 447 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HinfI 639 GACTC G^A[ACGT]TC KpnI 407 GGTACC GGTAC^C Ksp632I 1002 GAAGAG CTCTTC(1/4) MaeIII 1049 GTGAC ^GT[ACGT]AC NdeI 634 CATATG CA^TATG PflMI 198 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 639 GACTC GAGTC(4/5) RsaI 408 GTAC GT^AC SapI 1002 GAAGAGC GCTCTTC(1/4) SbfI 775 CCTGCAGG CCTGCA^GG SfaNI 929 GCATC GCATC(5/9) Sse8387I 775 CCTGCAGG CCTGCA^GG StuI 772 AGGCCT AGG^CCT TaqI 179 TCGA T^CGA Tsp45I 1049 GTGAC ^GT[GC]AC TthHB8I 179 TCGA T^CGA Van91I 198 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### ApaI 44 GGGCCC GGGCC^C 683 GGGCCC AspHI 757 GAGCTC G[AT]GC[AT]^C 1005 GAGCTC BbeI 12 GGCGCC GGCGC^C 216 GGCGCC BcnI 110 CCCGG CC^[GC]GG 115 CCCGG BsiHKAI 757 GAGCTC G[AT]GC[AT]^C 1005 GAGCTC BsmBI 514 GAGACG CGTCTC(1/5) 601 GAGACG BspMI 458 ACCTGC ACCTGC(4/8) 844 ACCTGC EaeI 2 TGGCCG [CT]^GGCC[GA] 117 CGGCCG KasI 12 GGCGCC G^GCGCC 216 GGCGCC MaeII 152 ACGT A^CGT 377 ACGT MboII 1002 GAAGA GAAGA(8/7) 901 TCTTC MslI 99 CACCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 138 CATCGCCGTG NarI 12 GGCGCC GG^CGCC 216 GGCGCC NciI 110 CCCGG CC^[GC]GG 115 CCCGG PpuMI 455 AGGACCT [GA]G^G[AT]CC[CT] 787 GGGACCT PstI 607 CTGCAG CTGCA^G 776 CTGCAG SacI 757 GAGCTC GAGCT^C 1005 GAGCTC SfcI 607 CTGCAG C^T[GA][CT]AG 776 CTGCAG SfoI 12 GGCGCC GGC^GCC 216 GGCGCC XcmI 357 CCAGAGGATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 914 CCATCCCCATCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AcyI AluI AvaII BanI BanII BbvI BglI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KspI MboI MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV SacII Sau3AI Sau96I ScrFI TseI TspRI