Restriction map for
pFN21AE4366
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglI BlnI BsaAI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MaeII MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 910 CGCG CG^CG
AflIII 728 ACATGT A^C[GA][CT]GT
AlwI 1098 GGATC GGATC(4/5)
ApaI 1055 GGGCCC GGGCC^C
AviII 380 TGCGCA TGC^GCA
BanI 1042 GGCGCC G^G[CT][GA]CC
BbeI 1042 GGCGCC GGCGC^C
BbsI 270 GAAGAC GAAGAC(2/6)
BciVI 979 GGATAC GTATCC(6/5)
BglII 893 AGATCT A^GATCT
BlpI 519 GCTCAGC GC^T[ACGT]AGC
BpmI 46 CTCCAG CTGGAG(16/14)
Bpu1102I 519 GCTCAGC GC^T[ACGT]AGC
BpuAI 270 GAAGAC GAAGAC(2/6)
BsaBI 765 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 305 GGTCTC GGTCTC(1/5)
BseRI 786 GAGGAG GAGGAG(10/8)
BsgI 1012 GTGCAG GTGCAG(16/14)
BsmBI 14 CGTCTC CGTCTC(1/5)
BspHI 608 TCATGA T^CATGA
BspLU11I 728 ACATGT A^CATGT
BspMI 942 GCAGGT ACCTGC(4/8)
BsrDI 570 CATTGC GCAATG(2/0)
BsrFI 854 GCCGGT [GA]^CCGG[CT]
BstEII 546 GGTCACC G^GT[ACGT]ACC
BstPI 546 GGTCACC G^GT[ACGT]ACC
BstUI 910 CGCG CG^CG
BstXI 752 CCAGTAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 519 GCTCAGC GC^T[ACGT]AGC
Cfr10I 854 GCCGGT [GA]^CCGG[CT]
DrdI 273 GACTCTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 546 GGTCACC G^GT[ACGT]ACC
EcoRI 845 GAATTC G^AATTC
EcoRV 579 GATATC GAT^ATC
FspI 380 TGCGCA TGC^GCA
HaeII 1042 GGCGCC [GA]GCGC^[CT]
KasI 1042 GGCGCC G^GCGCC
KspI 909 CCGCGG CCGC^GG
MamI 765 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 910 CGCG CG^CG
NarI 1042 GGCGCC GG^CGCC
RcaI 608 TCATGA T^CATGA
SacII 909 CCGCGG CCGC^GG
SfcI 165 CTACAG C^T[GA][CT]AG
SfoI 1042 GGCGCC GGC^GCC
SmlI 247 CTCAAG C^T[CT][GA]AG
SphI 233 GCATGC GCATG^C
TaqI 197 TCGA T^CGA
TthHB8I 197 TCGA T^CGA
XcmI 387 CCATGGCTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 478 GAATTT [GA]^AATT[CT]
845 GAATTC
AcyI 12 GGCGTC G[GA]^CG[CT]C
1042 GGCGCC
ApoI 478 GAATTT [GA]^AATT[CT]
845 GAATTC
BcnI 102 CCGGG CC^[GC]GG
841 CCGGG
BsaHI 12 GGCGTC G[GA]^CG[CT]C
1042 GGCGCC
BstYI 893 AGATCT [GA]^GATC[CT]
1098 GGATCT
EaeI 936 CGGCCA [CT]^GGCC[GA]
1092 CGGCCA
EcoNI 456 CCTAATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
779 CCTTCGGGAGG
FauI 538 GCGGG CCCGC(4/6)
1039 GCGGG
Hin1I 12 GGCGTC G[GA]^CG[CT]C
1042 GGCGCC
MboII 270 GAAGA GAAGA(8/7)
805 TCTTC
MflI 893 AGATCT [GA]^GATC[CT]
1098 GGATCT
NciI 102 CCGGG CC^[GC]GG
841 CCGGG
NcoI 387 CCATGG C^CATGG
1046 CCATGG
NspI 233 GCATGC [GA]CATG^[CT]
728 ACATGT
PflMI 585 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1046 CCATGGTATGG
PleI 273 GACTC GAGTC(4/5)
342 GAGTC
PpuMI 874 GGGACCC [GA]G^G[AT]CC[CT]
944 AGGTCCC
PvuII 87 CAGCTG CAG^CTG
1033 CAGCTG
TfiI 698 GATTC G^A[AT]TC
929 GAATC
Tsp509I 479 AATT ^AATT
846 AATT
Van91I 585 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1046 CCATGGTATGG
XhoII 893 AGATCT [GA]^GATC[CT]
1098 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI