(Circular) (Six-base) MAPSORT of: pFN21AE4366.seq Check: 2423 from: 1 to: 5854 pFN21AE4366 5854 bp With 182 enzymes: SgfI * March 28, 2012 13:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3664 278 Size: 53 83 186 936 2128 2468 Fragments arranged by size: 2468 2128 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3197 1937 Size: 1260 4594 Acc65I G'GTAC_C Cuts at: 3175 3175 Size: 5854 AclI AA'CG_TT Cuts at: 1563 3981 4354 1563 Size: 2418 373 3063 Fragments arranged by size: 3063 2418 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 3047 3917 5301 5569 1632 Size: 662 753 870 1384 268 1917 Fragments arranged by size: 1917 1384 870 753 662 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5657 AflIII A'CryG_T Cuts at: 1203 2733 4753 5730 1203 Size: 1530 2020 977 1327 Fragments arranged by size: 2020 1530 1327 977 AgeI A'CCGG_T Cuts at: 4685 4685 Size: 5854 AhdI GACnn_n'nnGTC Cuts at: 4583 4583 Size: 5854 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5854 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5854 AlwNI CAG_nnn'CTG Cuts at: 2521 3025 3106 3597 5169 2521 Size: 504 81 491 1572 3206 Fragments arranged by size: 3206 1572 504 491 81 ApaI G_GGCC'C Cuts at: 3064 3064 Size: 5854 ApaLI G'TGCA_C Cuts at: 3911 5067 3911 Size: 1156 4698 ApoI r'AATT_y Cuts at: 1493 2483 2850 3163 3389 3541 5650 1493 Size: 990 367 313 226 152 2109 1697 Fragments arranged by size: 2109 1697 990 367 313 226 152 AseI AT'TA_AT Cuts at: 160 1032 4408 160 Size: 872 3376 1606 Fragments arranged by size: 3376 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 AvaI C'yCGr_G Cuts at: 1870 1943 3179 1870 Size: 73 1236 4545 Fragments arranged by size: 4545 1236 73 BamHI G'GATC_C Cuts at: 3184 3551 3184 Size: 367 5487 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3047 3175 4630 618 Size: 325 217 627 1260 128 1455 1842 Fragments arranged by size: 1842 1455 1260 627 325 217 128 BanII G_rGCy'C Cuts at: 1391 2752 2833 3064 3089 3173 1391 Size: 1361 81 231 25 84 4072 Fragments arranged by size: 4072 1361 231 84 81 25 BbeI G_GCGC'C Cuts at: 3051 3051 Size: 5854 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 928 Size: 971 383 4500 Fragments arranged by size: 4500 971 383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4045 4079 1903 Size: 34 2108 34 3678 Fragments arranged by size: 3678 2108 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2978 3746 4956 2978 Size: 768 1210 3876 Fragments arranged by size: 3876 1210 768 BclI T'GATC_A Cuts at: 1548 1548 Size: 5854 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4465 136 Size: 107 122 71 631 605 2793 1525 Fragments arranged by size: 2793 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2898 5849 2898 Size: 2951 2903 BlpI GC'TnA_GC Cuts at: 2525 3271 2525 Size: 746 5108 Bme1580I G_kGCm'C Cuts at: 1790 3064 3915 5071 1790 Size: 1274 851 1156 2573 Fragments arranged by size: 2573 1274 1156 851 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5854 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2095 3585 4543 Size: 416 53 83 399 24 678 1490 958 Cuts at: 4543 442 Size: 1753 Fragments arranged by size: 1753 1490 958 678 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5854 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2817 2817 Size: 5854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 4514 1239 Size: 797 2478 2579 Fragments arranged by size: 2579 2478 797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 3979 4844 5142 2237 Size: 1742 865 298 2949 Fragments arranged by size: 2949 1742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2316 4517 882 Size: 617 817 2201 2219 Fragments arranged by size: 2219 2201 817 617 BsaAI yAC'GTr Cuts at: 493 1204 3645 493 Size: 711 2441 2702 Fragments arranged by size: 2702 2441 711 BsaBI GATnn'nnATC Cuts at: 2774 5848 2774 Size: 3074 2780 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 3048 3661 4043 Size: 53 83 186 936 485 1030 613 382 Cuts at: 4043 4678 275 Size: 635 1451 Fragments arranged by size: 1451 1030 936 635 613 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4287 4685 4959 5106 5517 4287 Size: 398 274 147 411 4624 Fragments arranged by size: 4624 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2910 2940 1178 Size: 30 165 30 1507 30 4092 Fragments arranged by size: 4092 1507 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2806 1186 Size: 1620 4234 BseYI C'CCAG_C Cuts at: 2499 2743 5057 5553 2499 Size: 244 2314 496 2800 Fragments arranged by size: 2800 2314 496 244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3038 1163 Size: 573 1302 3979 Fragments arranged by size: 3979 1302 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3304 4065 5093 1540 Size: 275 186 1303 761 1028 2301 Fragments arranged by size: 2301 1303 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3915 4000 5071 3915 Size: 85 1071 4698 Fragments arranged by size: 4698 1071 85 BsmI GAATG_Cn' Cuts at: 3365 3458 3365 Size: 93 5761 BsmBI CGTCTCn'nnnn_ Cuts at: 2025 2025 Size: 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2752 2833 3064 3089 3173 3915 4000 Size: 399 962 81 231 25 84 742 85 Cuts at: 4000 5071 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 962 742 399 231 85 84 81 25 BspEI T'CCGG_A Cuts at: 5517 5517 Size: 5854 BspHI T'CATG_A Cuts at: 2613 3743 5473 2613 Size: 1130 1730 2994 Fragments arranged by size: 2994 1730 1130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2938 3209 844 Size: 2094 271 3489 Fragments arranged by size: 3489 2094 271 BsrBI CCG'CTC Cuts at: 1394 1823 3741 1394 Size: 429 1918 3507 Fragments arranged by size: 3507 1918 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2574 4349 4523 65 Size: 1183 1326 1775 174 1396 Fragments arranged by size: 1775 1396 1326 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2859 4498 4685 1700 Size: 110 1049 1639 187 2869 Fragments arranged by size: 2869 1639 1049 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5854 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5854 BssSI C'ACGA_G Cuts at: 3914 4926 3914 Size: 1012 4842 BstAPI GCAn_nnn'nTGC Cuts at: 794 2521 2540 3146 794 Size: 1727 19 606 3502 Fragments arranged by size: 3502 1727 606 19 BstBI TT'CG_AA Cuts at: 4658 4658 Size: 5854 BstEII G'GTnAC_C Cuts at: 1184 2551 1184 Size: 1367 4487 BstXI CCAn_nnnn'nTGG Cuts at: 2764 2764 Size: 5854 BstYI r'GATC_y Cuts at: 1972 2898 3103 3184 3551 3936 3953 5405 5491 Size: 926 205 81 367 385 17 1452 86 Cuts at: 5491 5503 5849 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 926 385 367 346 205 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2392 2914 3051 513 Size: 552 939 388 522 137 3316 Fragments arranged by size: 3316 939 552 522 388 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2130 487 Size: 683 56 904 4211 Fragments arranged by size: 4211 904 683 56 BtsI GCAGTG_nn' Cuts at: 3370 4164 4184 3370 Size: 794 20 5040 Fragments arranged by size: 5040 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5854 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5819 DraI TTT'AAA Cuts at: 3158 3158 Size: 5854 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2284 4861 809 Size: 507 968 2577 1802 Fragments arranged by size: 2577 1802 968 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2941 3097 3301 Size: 54 1425 50 275 18 1111 156 204 Cuts at: 3301 4190 5819 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1111 889 275 204 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3301 1537 Size: 275 1489 4090 Fragments arranged by size: 4090 1489 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3784 1353 Size: 2431 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4441 4815 4961 387 Size: 1064 138 2852 374 146 1280 Fragments arranged by size: 2852 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2294 3047 3917 4514 5301 5569 Size: 393 404 258 753 870 597 787 268 Cuts at: 5569 1239 Size: 1524 Fragments arranged by size: 1524 870 787 753 597 404 393 268 258 EcoNI CCTnn'n_nnAGG Cuts at: 2465 2788 2465 Size: 323 5531 EcoO109I rG'GnC_Cy Cuts at: 2880 2950 3061 2880 Size: 70 111 5673 Fragments arranged by size: 5673 111 70 EcoRI G'AATT_C Cuts at: 2850 3163 3541 2850 Size: 313 378 5163 Fragments arranged by size: 5163 378 313 EcoRV GAT'ATC Cuts at: 2586 2586 Size: 5854 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2299 2299 Size: 5854 FspI TGC'GCA Cuts at: 2387 4360 2387 Size: 1973 3881 HaeII r_GCGC'y Cuts at: 1678 3051 5001 1678 Size: 1373 1950 2531 Fragments arranged by size: 2531 1950 1373 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2820 2852 2910 2942 4495 Size: 32 135 32 1248 32 58 32 1553 Cuts at: 4495 4527 4569 4601 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1553 1248 135 58 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3198 3444 669 Size: 988 281 1260 246 3079 Fragments arranged by size: 3079 1260 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5854 HpaI GTT'AAC Cuts at: 3444 3444 Size: 5854 KasI G'GCGC_C Cuts at: 3047 3047 Size: 5854 KpnI G_GTAC'C Cuts at: 3179 3179 Size: 5854 MfeI C'AATT_G Cuts at: 3453 3453 Size: 5854 MluI A'CGCG_T Cuts at: 5730 5730 Size: 5854 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2272 2361 2806 4968 5152 1305 Size: 967 89 445 2162 184 2007 Fragments arranged by size: 2162 2007 967 445 184 89 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4032 Fragments arranged by size: 4032 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3812 4171 4330 518 Size: 648 843 1803 359 159 2042 Fragments arranged by size: 2042 1803 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 2916 3040 3270 3947 5095 5340 1606 Size: 488 822 124 230 677 1148 245 2120 Fragments arranged by size: 2120 1148 822 677 488 245 230 124 NarI GG'CG_CC Cuts at: 3048 3048 Size: 5854 NcoI C'CATG_G Cuts at: 513 1065 2004 2392 3051 513 Size: 552 939 388 659 3316 Fragments arranged by size: 3316 939 659 552 388 NdeI CA'TA_TG Cuts at: 387 387 Size: 5854 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5854 NotI GC'GGCC_GC Cuts at: 3301 3301 Size: 5854 NspI r_CATG'y Cuts at: 1132 1229 2242 2737 3212 4757 1132 Size: 97 1013 495 475 1545 2229 Fragments arranged by size: 2229 1545 1013 495 475 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5854 PciI A'CATG_T Cuts at: 2733 4753 2733 Size: 2020 3834 PflMI CCAn_nnn'nTGG Cuts at: 1108 2596 3057 1108 Size: 1488 461 3905 Fragments arranged by size: 3905 1488 461 PmeI GTTT'AAAC Cuts at: 3158 3158 Size: 5854 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3923 3955 5436 5468 3923 Size: 32 1481 32 4309 Fragments arranged by size: 4309 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2880 2950 2880 Size: 70 5784 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5854 PsiI TTA'TAA Cuts at: 2489 3424 2489 Size: 935 4919 PspOMI G'GGCC_C Cuts at: 3060 3060 Size: 5854 PstI C_TGCA'G Cuts at: 830 3206 830 Size: 2376 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5854 PvuII CAG'CTG Cuts at: 2094 3040 2094 Size: 946 4908 SacII CC_GC'GG Cuts at: 2917 2917 Size: 5854 SalI G'TCGA_C Cuts at: 1936 3196 1936 Size: 1260 4594 SanDI GG'GwC_CC Cuts at: 2880 2880 Size: 5854 SbfI CC_TGCA'GG Cuts at: 3206 3206 Size: 5854 ScaI AGT'ACT Cuts at: 4102 5814 4102 Size: 1712 4142 SfcI C'TryA_G Cuts at: 826 1045 2170 3202 4337 5018 5209 826 Size: 219 1125 1032 1135 681 191 1471 Fragments arranged by size: 1471 1135 1125 1032 681 219 191 SfoI GGC'GCC Cuts at: 3049 3049 Size: 5854 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5854 SmaI CCC'GGG Cuts at: 1872 3181 1872 Size: 1309 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 3958 4859 5121 820 Size: 197 926 309 1706 901 262 1553 Fragments arranged by size: 1706 1553 926 901 309 262 197 SnaBI TAC'GTA Cuts at: 493 3645 493 Size: 3152 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5854 SphI G_CATG'C Cuts at: 1132 2242 3212 1132 Size: 1110 970 3774 Fragments arranged by size: 3774 1110 970 SspI AAT'ATT Cuts at: 5 52 3778 5 Size: 47 3726 2081 Fragments arranged by size: 3726 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5854 StyI C'CwwG_G Cuts at: 513 1065 2004 2392 2868 2964 3051 3293 513 Size: 552 939 388 476 96 87 242 3074 Fragments arranged by size: 3074 939 552 476 388 242 96 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2357 2405 3881 3898 4051 4236 Size: 1255 417 360 48 1476 17 153 185 Cuts at: 4236 4681 325 Size: 445 1498 Fragments arranged by size: 1498 1476 1255 445 417 360 185 153 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4100 5812 96 Size: 274 80 33 51 1443 2123 1712 138 Fragments arranged by size: 2123 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5814 5846 5814 Size: 32 5822 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5854 XbaI T'CTAG_A Cuts at: 3190 3190 Size: 5854 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2399 2399 Size: 5854 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5854 XmaI C'CCGG_G Cuts at: 1870 3179 1870 Size: 1309 4545 XmnI GAAnn'nnTTC Cuts at: 3983 3983 Size: 5854 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3662 276 Size: 53 83 186 936 2128 2468 Fragments arranged by size: 2468 2128 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI