(Linear) (Six-base) MAPSORT of: KIEE4366.seq Check: 4996 from: 1 to: 1149 KIEE4366.orf With 182 enzymes: SgfI * March 22, 2012 17:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 1042 1149 Size: 289 753 107 Fragments arranged by size: 753 289 107 AflIII A'CryG_T Cuts at: 0 728 1149 Size: 728 421 AlwNI CAG_nnn'CTG Cuts at: 0 516 1020 1101 1149 Size: 516 504 81 48 Fragments arranged by size: 516 504 81 48 ApaI G_GGCC'C Cuts at: 0 1059 1149 Size: 1059 90 ApoI r'AATT_y Cuts at: 0 478 845 1149 Size: 478 367 304 Fragments arranged by size: 478 367 304 BanI G'GyrC_C Cuts at: 0 1042 1149 Size: 1042 107 BanII G_rGCy'C Cuts at: 0 747 828 1059 1084 1149 Size: 747 81 231 25 65 Fragments arranged by size: 747 231 81 65 25 BbeI G_GCGC'C Cuts at: 0 1046 1149 Size: 1046 103 BbsI GAAGACnn'nnnn_ Cuts at: 0 277 1149 Size: 277 872 BciVI GTATCCnnnnn_n' Cuts at: 0 973 1149 Size: 973 176 BglII A'GATC_T Cuts at: 0 893 1149 Size: 893 256 BlpI GC'TnA_GC Cuts at: 0 520 1149 Size: 520 629 Bme1580I G_kGCm'C Cuts at: 0 1059 1149 Size: 1059 90 BmrI ACTGGGnnnn_n' Cuts at: 0 90 1149 Size: 90 1059 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 812 1149 Size: 812 337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 1149 Size: 31 1118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 1149 Size: 232 917 BsaI GGTCTCn'nnnn_ Cuts at: 0 311 1149 Size: 311 838 BsaBI GATnn'nnATC Cuts at: 0 769 1149 Size: 769 380 BsaHI Gr'CG_yC Cuts at: 0 13 1043 1149 Size: 13 1030 106 Fragments arranged by size: 1030 106 13 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 905 935 1149 Size: 905 30 214 Fragments arranged by size: 905 214 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 801 1149 Size: 801 348 BseYI C'CCAG_C Cuts at: 0 494 738 1149 Size: 494 244 411 Fragments arranged by size: 494 411 244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1033 1149 Size: 1033 116 BsmBI CGTCTCn'nnnn_ Cuts at: 0 20 1149 Size: 20 1129 Bsp1286I G_dGCh'C Cuts at: 0 747 828 1059 1084 1149 Size: 747 81 231 25 65 Fragments arranged by size: 747 231 81 65 25 BspHI T'CATG_A Cuts at: 0 608 1149 Size: 608 541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 933 1149 Size: 933 216 BsrDI GCAATG_nn' Cuts at: 0 569 1149 Size: 569 580 BsrFI r'CCGG_y Cuts at: 0 854 1149 Size: 854 295 BstAPI GCAn_nnn'nTGC Cuts at: 0 516 535 1141 1149 Size: 516 19 606 8 Fragments arranged by size: 606 516 19 8 BstEII G'GTnAC_C Cuts at: 0 546 1149 Size: 546 603 BstXI CCAn_nnnn'nTGG Cuts at: 0 759 1149 Size: 759 390 BstYI r'GATC_y Cuts at: 0 893 1098 1149 Size: 893 205 51 Fragments arranged by size: 893 205 51 BtgI C'CryG_G Cuts at: 0 387 909 1046 1149 Size: 387 522 137 103 Fragments arranged by size: 522 387 137 103 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 125 1149 Size: 125 1024 DrdI GACnn_nn'nnGTC Cuts at: 0 279 1149 Size: 279 870 EaeI y'GGCC_r Cuts at: 0 936 1092 1149 Size: 936 156 57 Fragments arranged by size: 936 156 57 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 289 1042 1149 Size: 31 258 753 107 Fragments arranged by size: 753 258 107 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 460 783 1149 Size: 460 323 366 Fragments arranged by size: 460 366 323 EcoO109I rG'GnC_Cy Cuts at: 0 875 945 1056 1149 Size: 875 70 111 93 Fragments arranged by size: 875 111 93 70 EcoRI G'AATT_C Cuts at: 0 845 1149 Size: 845 304 EcoRV GAT'ATC Cuts at: 0 581 1149 Size: 581 568 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 294 1149 Size: 294 855 FspI TGC'GCA Cuts at: 0 382 1149 Size: 382 767 HaeII r_GCGC'y Cuts at: 0 1046 1149 Size: 1046 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 815 847 905 937 1149 Size: 815 32 58 32 212 Fragments arranged by size: 815 212 58 32 32 KasI G'GCGC_C Cuts at: 0 1042 1149 Size: 1042 107 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 267 356 801 1149 Size: 267 89 445 348 Fragments arranged by size: 445 348 267 89 MspA1I CmG'CkG Cuts at: 0 89 911 1035 1149 Size: 89 822 124 114 Fragments arranged by size: 822 124 114 89 NarI GG'CG_CC Cuts at: 0 1043 1149 Size: 1043 106 NcoI C'CATG_G Cuts at: 0 387 1046 1149 Size: 387 659 103 Fragments arranged by size: 659 387 103 NspI r_CATG'y Cuts at: 0 237 732 1149 Size: 237 495 417 Fragments arranged by size: 495 417 237 PciI A'CATG_T Cuts at: 0 728 1149 Size: 728 421 PflMI CCAn_nnn'nTGG Cuts at: 0 591 1052 1149 Size: 591 461 97 Fragments arranged by size: 591 461 97 PpuMI rG'GwC_Cy Cuts at: 0 875 945 1149 Size: 875 70 204 Fragments arranged by size: 875 204 70 PsiI TTA'TAA Cuts at: 0 484 1149 Size: 484 665 PspOMI G'GGCC_C Cuts at: 0 1055 1149 Size: 1055 94 PvuII CAG'CTG Cuts at: 0 89 1035 1149 Size: 89 946 114 Fragments arranged by size: 946 114 89 SacII CC_GC'GG Cuts at: 0 912 1149 Size: 912 237 SanDI GG'GwC_CC Cuts at: 0 875 1149 Size: 875 274 SfcI C'TryA_G Cuts at: 0 165 1149 Size: 165 984 SfoI GGC'GCC Cuts at: 0 1044 1149 Size: 1044 105 SmlI C'TyrA_G Cuts at: 0 247 1149 Size: 247 902 SphI G_CATG'C Cuts at: 0 237 1149 Size: 237 912 StyI C'CwwG_G Cuts at: 0 387 863 959 1046 1149 Size: 387 476 96 87 103 Fragments arranged by size: 476 387 103 96 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 352 400 1149 Size: 352 48 749 Fragments arranged by size: 749 352 48 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 394 1149 Size: 394 755 Enzymes that do cut: AcuI AflIII AlwNI ApaI ApoI BanI BanII BbeI BbsI BciVI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI DrdI EaeI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I KasI MmeI MspA1I NarI NcoI NspI PciI PflMI PpuMI PsiI PspOMI PvuII SacII SanDI SfcI SfoI SmlI SphI StyI TaqII XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI BglI BmgBI BmtI Bpu10I BsaAI BsaWI BsiEI BsiHKAI BsiWI BsmI BspEI BsrBI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI PspXI PsrI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI